| Budget Amount *help |
¥18,850,000 (Direct Cost: ¥14,500,000、Indirect Cost: ¥4,350,000)
Fiscal Year 2014: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2013: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2012: ¥9,880,000 (Direct Cost: ¥7,600,000、Indirect Cost: ¥2,280,000)
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| Outline of Final Research Achievements |
In this study, I aim at a development of novel RNAi medicine based on shRNA expression mini-gene. As a result, I succeeded to prepare the mini-gene via PCR amplification using 2’-deoxy-4’-thionucleoside triphosphates (dSNTPs). In addition, I also developed a practical method to cyclize the resulting mini-gene.
The mini-gene showed potent RNAi activity both in vitro and in vivo. In addition, innate immune responses such as type-I INF and inflammatory cytokines were hardly induced by administration of the mini-gene in vivo.
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