Claudin-1 as a target for modulation of epidermal barriers

• Project/Area Number: 24390042
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Medical pharmacy
• Research Institution: Osaka University
• Principal Investigator: KONDOH Masuo 大阪大学, 薬学研究科(研究院), 准教授 (50309697)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥18,590,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥4,290,000); Fiscal Year 2014: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000); Fiscal Year 2013: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000); Fiscal Year 2012: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
• Keywords: タイトジャンクション / 非侵襲性投与 / Claudin / Tight junction / 上皮バリア / tight junction / claudin

Outline of Final Research Achievements

Epithelium plays pivotal roles in barriers that separate the inside of the body from the outside environment. Passage across epithelial barriers is the first step in drug absorption. Epidermal absorption is an ideal route for drug administration because it is non-invasive and easily controlled. Claudin-1 (CLDN1) seals tight junctions in the stratum granulosum, but whether modulation of CLDN1 enhances epidermal permeability is unclear. We developed anti-CLDN1 antibody, and we investigated the effect of an anti-CLDN1 antibody on the epidermal epithelial barrier by using human epidermal keratinocytes (NHEK), a popular in vitro model of human epithelial cell sheets. Treatment of NHEK monolayers with anti-CLDN1 antibody decreased the integrity of tight junctions between adjacent cells and enhanced the permeation of solutes across the cell sheets. These findings suggest that CLND1 may be a target for specific modulation of epidermal barrier.

Research Products

• [Journal Article] Homoharringtonine increases intestinal epithelial permeability by modulating specific claudin isoforms in Caco-2 cell monolayers (2015)
  • Author(s): Akihiro Watari, Maki Hasegawa, Kiyohito Yagi, Masuo Kondoh
  • Journal Title: European Journal of Pharmaceutics and Biopharmaceutics Volume: 89 Pages: 232-238
  • DOI: 10.1016/j.ejpb.2014.12.012
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Discovery of anti-claudin-1 antibodies as candidate therapeutics against hepatitis C virus (2015)
  • Author(s): Mayo Yamashita, Manami Iida, Minoru Tada, Yohitaka Shirasago, Masayoshi Fukasawa, Shotaro Nagase, Akihiro Watari, Akiko Ishii-Watabe, Kiyohito Yagi, Masuo Kondoh
  • Journal Title: Journal of Pharmacology and Experimental Therapeutics Volume: 353 Pages: 112-118
  • DOI: 10.1124/jpet.114.217653
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Monoclonal antibodies against extracellular domains of claudin-1 block hepatitis C virus infection in a mouse model (2015)
  • Author(s): Masayoshi Fukasawa, Shotaro Nagase, Yoshitaka Shirasago, Manami Iida, Mayo Yamashita, Kohki Endo, Kiyohito Yagi, Tetsuro Suzuki, Takaji Wakita, Kentaro Hanada, Hiroki Kuniyasu, Masuo Kondoh
  • Journal Title: J Virol. Volume: 89 Pages: 4866-4879
  • DOI: 10.1128/jvi.03676-14
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Tissue distribution and safety evaluation of a claudin-targeting molecule, the C-terminal fragment of Clostridium perfringens enterotoxin (2014)
  • Author(s): Xiangru Li, Rie Saeki, Akihiro Watari, Kiyohito Yagi, Masuo Kondoh
  • Journal Title: Eur. J. Pharm. Sci. Volume: 52 Pages: 132-137
  • DOI: 10.1016/j.ejps.2013.10.018
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Development of an anti-claudin-3 and -4 bispecific monoclonal antibody for cancer diagnosis and therapy (2014)
  • Author(s): Xiangru Li, Manami Iida, Minoru Tada, Akihiro Watari, Yumi Kawahigashi, Yuka Kimura, Taku Yamashita, Akiko Ishii-Watabe, Tadayuki Uno, Masayoshi Fukasawa, Hiroki Kuniyasu, Kiyohito Yagi, Masuo Kondoh
  • Journal Title: Journal of Pharmacology and Experimental Therapeutics Volume: 351 Pages: 206-213
  • DOI: 10.1124/jpet.114.216911
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Tissue distribution and safety evaluation of a claudin-targeting molecule, the C-terminal fragment of Clostridium perfringens enterotoxin (2014)
  • Author(s): Xiangru Li, Rie Saeki, Akihiro Watari, Kiyohito Yagi, Masuo Kondoh
  • Journal Title: Eur J Pharm Sci Volume: 52 Pages: 132-137
  • Peer Reviewed
• [Journal Article] The new era of epithelium-targeted drug development (2014)
  • Author(s): Yoshimi Shimizu, Shotaro Nagase, Kiyohito Yagi, Masuo Kondoh
  • Journal Title: Yakugakuzasshi Volume: in press
  • NAID: 130004756437
  • Peer Reviewed
• [Journal Article] The cutting edge of epithelium-targeted drug development (2014)
  • Author(s): Masuo Kondoh, Jun Kunisawa
  • Journal Title: Yakugakuzasshi Volume: in press
  • NAID: 130004756438
• [Journal Article] A baculoviral display system to assay viral entry (2013)
  • Author(s): Manami Iida, Takeshi Yoshida, Akihiro Watari, Kiyohito Yagi, Takao Hamakubo, Masuo Kondoh
  • Journal Title: Biol Pharm Bull Volume: 36 Pages: 1867-1869
  • Peer Reviewed
• [Journal Article] Recent advances in claudin-targeting technology (2013)
  • Author(s): Shotaro Nagase, Ryo Doyama, Kiyohito Yagi, Masuo Kondoh
  • Journal Title: Biol Pharm Bull Volume: 36 Pages: 708-714
  • Peer Reviewed
• [Journal Article] Claudinを標的とした創薬研究の最前線 (2013)
  • Author(s): 飯田愛未、八木清仁、近藤昌夫
  • Journal Title: 膜 Volume: 38 Pages: 174-180
  • NAID: 10031190688
  • Peer Reviewed
• [Journal Article] Percolation Analysis in Electrical Conductivity of MDCK and Caco-2 Cells Permeat ion-Enhanc ing Agents (2013)
  • Author(s): Makiko Washiyama, Naoya Koizumi, Makiko Fujii, Masuo Kondoh, Kiyohito Yagi, Yoshiteru Watanabe
  • Journal Title: Biol Pharm Bull Volume: 36 Pages: 384-384
  • Peer Reviewed
• [Journal Article] Recent advances ln claudin-target ing technology (2013)
  • Author(s): Shotaro Nagase, Ryo Doyama, Kiyohito Yagi, Masuo Kondoh
  • Journal Title: Biol Pharm Bull Volume: 36 Pages: 708-714
  • Peer Reviewed
• [Journal Article] A simple reporter assay for screening claudin-4 modulators (2012)
  • Author(s): Akihiro Watari, Kiyohito Yagi, Masuo Kondoh
  • Journal Title: Biochem Biophys Res Commun Volume: 426 Pages: 454-460
  • Peer Reviewed
• [Journal Article] Comparison of mucosal absorption-enhancing activity between a claudin-3/ -binder and a broadly specific claudirt binder (2012)
  • Author(s): Koji Matsuhisa, Masuo Kondoh, Hidehiko Suzuki, Kiyohito Yagi
  • Journal Title: Biochem Biophys Res Commun Volume: 423 Pages: 229-233
  • Peer Reviewed
• [Journal Article] Oil-in-water emulsion lot ion providing controlled release using 2-methacryloyloxyethyl phosphorylcholine n-butyl methacrylate copolymer as cmulsifier (2012)
  • Author(s): Akiko Ishikawa, Makiko Fujii, Kumi Mori moto, Naoya Koizumi, Masuo Kondoh, Yoshitoru Watanabe
  • Journal Title: Pharm Sci Volume: 2 Pages: 16-22
  • Peer Reviewed
• [Journal Article] The application of an alanine-substituted mutant of the C-terminal fragment of Clostridium perfringens enterotoxin as a mucosal vaccine in mice (2012)
  • Author(s): Hidehiko Suzuki, Masuo Kondoh, Hideki Kakutani, Seiji Yamane, Hiroshi Uchida, Takao Hamakubo, Kiyohito Yagi
  • Journal Title: Biomatcrials Volume: 33 Pages: 317-324
  • Peer Reviewed
• [Journal Article] Creatioll and biochemical analysis of a broad-specific claudin binder (2012)
  • Author(s): Azusa Takahashi, Yumiko Saito, Masuo Kondoh, Kyohei Matsushi ta, Susanne M. Krug, Hidehiko Suzuki, Hirofumi Tsujino, Xiangru Li, Hiroshi Aoyama, Koji Matsuhisa, Tadayuki Uno, Michael Fromm, Takao Hamakubo, Kiyohito Yagi
  • Journal Title: Biomatcrials Volume: 33 Pages: 3464-3474
  • Peer Reviewed
• [Journal Article] Pathological changes in tight junctions and potential applicat ions into therapi e (2012)
  • Author(s): Azusa Takahashi, Masuo Kondoh, Hidehiko Suzuki, Akihiro Watari, Kiyohito Yagi
  • Journal Title: Drug Discov Today Volume: 17 Pages: 727-732
  • Peer Reviewed
• [Journal Article] Proof of concept for claudin-target.ed drug development (2012)
  • Author(s): Hidehiko Suzuki, Masuo Kondoh, Azusa Takahashi, Kiyohito Yagi
  • Journal Title: Ann NY Acad Sci Volume: 1258 Pages: 65-70
  • Peer Reviewed
• [Journal Article] Spiral progression in the development of absorption enhancers based on the biology of tight junctions (2012)
  • Author(s): Masuo Kondoh, Azusa Takahashi, Kiyohito Yagi
  • Journal Title: Adv Drug Deliv Rev Volume: 64 Pages: 515-522
  • Peer Reviewed
• [Presentation] Anti-human claudin-1 monoclonal antbodies as anti-Hepatitis C Virus agents (2014)
  • Author(s): M. Iida, M. Yamashita, S. Nagase, M. Tada, Y. Shirasago, M. Fukasawa, A.Watari, A.Ishii-Watabe, K. Yagi, M. Kondoh
  • Organizer: Antibody Engineering & Therapeutics 2014
  • Place of Presentation: Huntington Beach, CA, U.S.A.
  • Year and Date: 2014-12-07 – 2014-12-11
  • Invited
• [Presentation] Epitope mapping of an anti-claudin-1 monoclonal antibody and its application to preclinical drug discovery (2014)
  • Author(s): T. Hata, S. Nagase, Y. Shirasago, M. Fukasawa, A. Watari, K. Yagi, M. Kondoh
  • Organizer: Antibody Engineering & Therapeutics 2014
  • Place of Presentation: Huntington Beach, CA, U.S.A.
  • Year and Date: 2014-12-07 – 2014-12-11
  • Invited
• [Presentation] Development of a Novel Bispecific Anti-Claudin Antibody and its Anti-Tumor Activity (2014)
  • Author(s): Y. Kimura, X. Li, M. Iida, M. Tada, A. Watabe-Ishii, M. Fukasawa, Y. Kawahigashi, A. Watari, K. Yagi, M. Kondoh
  • Organizer: Antibody Engineering & Therapeutics 2014
  • Place of Presentation: Huntington Beach, CA, U.S.A.
  • Year and Date: 2014-12-07 – 2014-12-11
  • Invited
• [Presentation] Paracellular or transcellular? Promising approach for oral delivery of macromolecules (2014)
  • Author(s): Masuo Kondoh, Kiyohito Yagi
  • Organizer: 2014 AAPS Annual Meeting and Exposition
  • Place of Presentation: SanDiego, U.S.A.
  • Year and Date: 2014-11-05
  • Invited
• [Presentation] In vivo inhibition of hepatitis C virus infection by anti-claudin-1 monoclonal antibodies (2014)
  • Author(s): M.Iida, S.Nagase, M.Yamashita, Y.Shirasago, M.Fukasawa, M.Tada, A.Ishii, A.Watari, K.Yagi, and M.Kondoh
  • Organizer: The 41st Annual Meeting & Exposition of the controlled Release Society
  • Place of Presentation: Chicago, U.S.A.
  • Year and Date: 2014-07-13 – 2014-07-16
  • Invited
• [Presentation] In vivo inhibition of hepatitis C virus infection by anti-Claudin-1 monoconal antibodies (2014)
  • Author(s): M.Iida, S.Nagase, M.Yamashita, Y.Shirasago, M.Fukasawa, M.Tada, A.Ishii, A.Watari,
  • Organizer: Experimental Biology 2014
  • Place of Presentation: SanDiego,U.S.A.
  • Year and Date: 2014-04-26 – 2014-04-30
  • Invited
• [Presentation] Modulation of the epithelial bariier by a fragment of Clostridium perfringens iota-toxin (2014)
  • Author(s): D.Iguchi, T.Hayaishi, S.M. Krug, A.Watari, M.Fromm, K.Yagi and M.Kondoh
  • Organizer: Experimental Biology 2014
  • Place of Presentation: SanDiego,U.S.A.
  • Year and Date: 2014-04-26 – 2014-04-30
  • Invited
• [Presentation] Development and anti-tumor activities of claudin-4-specific monoclonal antibodies (2014)
  • Author(s): Y.Kawahigashi, X.Li, H.Kuniyasu, M.Fukasawa, M.Tada, A.Ishii, A.Watari, K.Yagi and
  • Organizer: Experimental Biology 2014
  • Place of Presentation: SanDiego,U.S.A.
  • Year and Date: 2014-04-26 – 2014-04-30
  • Invited
• [Presentation] Tumor-targeting and anti-tumor activity of anti-claucin-3/-4 antibody (2014)
  • Author(s): Y.Kimura, X.Li, M.Iida, M.Tada, A.Ishii, A.Watari, M.Fukasawa, K.Yagi and M.Kondoh
  • Organizer: Experimental Biology 2014
  • Place of Presentation: SanDiego,U.S.A.
  • Year and Date: 2014-04-26 – 2014-04-30
  • Invited
• [Presentation] Claudin-1 as a target for modulation of epidermal barriers (2014)
  • Author(s): M.Nakajima, M.Yamashita, A.Watari, K.Yagi, M.Fukasawa, and M.Kondoh
  • Organizer: Experimental Biology 2014
  • Place of Presentation: SanDiego,U.S.A.
  • Year and Date: 2014-04-26 – 2014-04-30
  • Invited
• [Presentation] 上皮を標的とした創薬基盤研究～Claudin-4抗体の作製および抗腫瘍活性解析～ (2014)
  • Author(s): 川東祐美、李相儒、國安弘基、多田稔、石井明子、深澤征義、渡利彰浩、近藤昌夫、八木清仁
  • Organizer: 日本薬学会第134年会
  • Place of Presentation: 熊本
• [Presentation] 上皮を標的とした創薬基盤研究～抗claudin-1抗体のエピトープ・交叉性解析～ (2014)
  • Author(s): 畑智幸、長瀬翔太郎、白砂圭崇、深澤征義、渡利彰浩、近藤昌夫、八木清仁
  • Organizer: 日本薬学会第134年会
  • Place of Presentation: 熊本
• [Presentation] 上皮を標的とした創薬基盤研究～Dual specific claudin-3/-4抗体の動態特性および抗癌活性解析～ (2014)
  • Author(s): 木村友香、李相儒、飯田愛未、多田稔、石井明子、國安弘基、深澤征義、渡利彰浩、近藤昌夫、八木清仁
  • Organizer: 日本薬学会第134年会
  • Place of Presentation: 熊本
• [Presentation] 上皮を標的とした創薬基盤研究～Tricellular tight junction制御法の開発～ (2014)
  • Author(s): 井口大輔、早石知浩、Susanne M. Krug、Michael Fromm、渡利彰浩、近藤昌夫、八木清仁
  • Organizer: 日本薬学会第134年会
  • Place of Presentation: 熊本
• [Presentation] 上皮を標的とした創薬基盤研究～Claudin-1を標的とした表皮バリア制御の可能性～ (2014)
  • Author(s): 中嶋美咲、山下真代、渡利彰浩、深澤征義、近藤昌夫、八木清仁
  • Organizer: 日本薬学会第134年会
  • Place of Presentation: 熊本
• [Presentation] Effect of claudin on cytochrome P450 activity (2013)
  • Author(s): Shimizu, Y.; Kondoh, M.; Watari, A.; Fukasawa, M.; Yagi, K.
  • Organizer: Experimental Biology 2013
  • Place of Presentation: Boston, MA, USA
• [Presentation] Characterization of scFv libraries derived from mice differently immunized with claudin. (2013)
  • Author(s): Yamashita, M.; Nagase, S.; Takahashi, A.; Iwanari, H.; Kondoh, M.; Watari, A.; Hamakubo, T.; Yagi, K
  • Organizer: Experimental Biology 2013
  • Place of Presentation: Boston, MA, USA
• [Presentation] Development of anti-claudin-1 ligand (2013)
  • Author(s): Nagase, S.; Yamashita, M.; Iida, M.; Watari, A.; Kondoh, M.; Fukasawa, M.; Yagi, K
  • Organizer: Experimental Biology 2013
  • Place of Presentation: Boston, MA, USA
• [Presentation] A toxicological evaluation of the C-terminal fragment of Clostridium perfringens enterotoxin as a claudin-3/-4 binder (2013)
  • Author(s): Iida, M.; Kondoh, M.; Watari, A.; Yagi, K
  • Organizer: Experimental Biology 2013
  • Place of Presentation: Boston, MA, USA
• [Presentation] Tissue-distributio of claudin-3/-4 binder, the C-terminal fragment of Clostridium perfringens enterotoxin, in mice (2013)
  • Author(s): Li, X.; Kondoh, M.; Watari, A.; Yagi, K
  • Organizer: Experimental Biology 2013
  • Place of Presentation: Boston, MA, USA
• [Presentation] Bicellular or tricellular tight junction binders and their pharmaceutical activities (2013)
  • Author(s): Kondoh, M.; Yagi, K
  • Organizer: Gordon research seminar,
  • Place of Presentation: Lucca, Italy
• [Presentation] Bicellular or tricellular tight junction binders and their pharmaceutical activities (2013)
  • Author(s): Kondoh, M.; Yagi, K
  • Organizer: Gordon research conference
  • Place of Presentation: Lucca, Italy
• [Presentation] Claudin-1-specific monoclonal antibodies and their inhibition of hepatitis C virus infection (2013)
  • Author(s): Nagase, S.; Yamashita, M.; Iida, M.; Watari, A.; Yagi, K.; Kondoh, M
  • Organizer: 40th Annual Meeting & Exposition of the Controlled Release Society
  • Place of Presentation: Honolulu, Hawaii, USA
• [Presentation] An improved claudin-targeting mucosal vaccine using a double alanine-substituted mutant of the C-terminal fragment of Clostridium perfringens enterotoxin (2013)
  • Author(s): Suzuki, H.; Kunisawa, J.; Yamashita, M.; Watari, A.; Yagi, K.; Kondoh, M
  • Organizer: 40th Annual Meeting & Exposition of the Controlled Release Society
  • Place of Presentation: Honolulu, Hawaii, USA
• [Presentation] Claudin-1-specific monoclonal antibodies and their inhibition of hepatitis C virus infection (2013)
  • Author(s): Nagase, S.; Yamashita, M.; Iida, M.; Shirasago, Y.; Fukasawa, M.; Watari, A.; Yagi, K.; Kondoh, M
  • Organizer: ILCA 2013: International Liver Cancer Association Seventh Annual Conference
  • Place of Presentation: Washington, D.C. USA
• [Presentation] Inhibition of hepatitis C virus infection by mouse anti-claudin 1 monoclonal antibodies (2013)
  • Author(s): Fukasawa, M.; Nagase, S.; Yamashita, M.; Iida, M.; Shirasago, Y.; Watari, A.; Suzuki, T.; Wakita, T.; Yagi, K.; Kondoh, M
  • Organizer: HCV2013 20th International Symposium on Hepatittis C virus and Relate viruses 2013
  • Place of Presentation: Melbourne, Australia.
• [Presentation] Development of monoclonal antibodies to the extracellular loop regions of claudin-1 and their application to HCV therapy (2013)
  • Author(s): Nagase, S.; Yamashita, M.; Iida, M.; Fukasawa, M.; Watari, A.; Yagi, K.; Kondoh, M
  • Organizer: 2013 AAPS Annual Meeting and Exposition
  • Place of Presentation: San Antonio, TX, USA
• [Presentation] Creation of a broadly specific claudin binder and its absorption-enhancing activity (2013)
  • Author(s): Doyama, R.; Matsushita, K.; Takahashi, A.; Matsuhisa, K.; Watari, A.; Hamakubo, T.; Yagi, K.; Kondoh, M
  • Organizer: 2013 AAPS Annual Meeting and Exposition
  • Place of Presentation: San Antonio, TX, USA
• [Presentation] Development of claudin-4-specific monoclonal antibodies and their anti-tumor activities (2013)
  • Author(s): Iida, M.; Li, X.; Kuniyasu, H.; Fukasawa, M.; Tada, M.; Ishii, A.; Watari, A.; Yagi, K.; Kondoh, M.
  • Organizer: Antibody Engineering & Terapeutics 2013
  • Place of Presentation: Huntington Beach, CA, USA
• [Presentation] Claudin-1-specific monoclonal antibodies and their inhibitory activity against hepatitis C virus infection (2013)
  • Author(s): Nagase, S.; Yamashita, M.; Iida, M.; Shirasago, Y.; Fukasawa, M.; Watari, A.; Yagi, K.; Kondoh, M.
  • Organizer: Antibody Engineering & Terapeutics 2013
  • Place of Presentation: Huntington Beach, CA, USA
• [Presentation] Tissue-distribution and safety evaluation of a claudin-3/-4 binder in mice (2013)
  • Author(s): Li, X.; Watari, A.; Yagi, K.; Kondoh, M
  • Organizer: 53rd Annual meeting of the American society for cell biology
  • Place of Presentation: New Orleans, LO, USA
• [Presentation] In vitro and in vivo inhibition of hepatitis C virus infection by mouse anti-human claudin 1 monoclonal antibodies (2013)
  • Author(s): Fukasawa, M.; Nagase, S.; Yamashita, M.; Iida, M.; Shirasago, Y.; Hanada, K.; Watari, A.; Yagi, K.; Kondoh, M
  • Organizer: 53rd Annual meeting of the American society for cell biology
  • Place of Presentation: New Orleans, LO, USA
• [Presentation] Identification of chemical compounds that modulate claudin-4 expression by cell-based screening (2013)
  • Author(s): Watari, A.; Hasegawa, M.; Yagi, K.; Kondoh, M
  • Organizer: 53rd Annual meeting of the American society for cell biology
  • Place of Presentation: New Orleans, LO, USA
• [Presentation] 上皮バリアを標的とした創薬研究の新展開 (2013)
  • Author(s): 近藤昌夫、八木清仁
  • Organizer: 日本膜学会第35年会
  • Place of Presentation: 東京
  • Invited
• [Presentation] 上皮細胞を標的とした創薬基盤研究第１報 ～claudin-1特異性抗体の創製～ (2013)
  • Author(s): 長瀬翔太郎、山下真代、、飯田愛未、渡利彰浩、近藤昌夫、深澤征義、多田稔、石井明子、八木清仁
  • Organizer: 第29回日本DDS学会
  • Place of Presentation: 京都
• [Presentation] 上皮細胞を標的とした創薬基盤研究第2報 ～claudin-1抗体のC型肝炎治療への応用～ (2013)
  • Author(s): 飯田愛未、長瀬翔太郎、山下真代、近藤昌夫、八木清仁、國安弘基、深澤征義
  • Organizer: 第29回日本DDS学会
  • Place of Presentation: 京都
• [Presentation] 上皮細胞を標的とした創薬基盤研究第３報 ～claudin-4特異性抗体の創製～ (2013)
  • Author(s): 清水芳実、李相儒、渡利彰浩、近藤昌夫、深澤征義、多田稔、石井明子、國安弘基、八木清仁
  • Organizer: 第29回日本DDS学会
  • Place of Presentation: 京都
• [Presentation] 上皮バリア制御による薬物吸収促進研究の新展開 (2013)
  • Author(s): 近藤昌夫、八木清仁
  • Organizer: 第29回日本DDS学会
  • Place of Presentation: 京都
  • Invited
• [Presentation] ウエルシュ菌イオタ毒素を用いた tricellular tight junction制御法の開発 (2013)
  • Author(s): 早石知浩、渡利彰浩、近藤昌夫、八木清仁
  • Organizer: 第60回トキシンシンポジウム
  • Place of Presentation: 兵庫
• [Presentation] Claudinを標的とした粘膜吸収促進法の開発 (2013)
  • Author(s): 飯田愛未、鈴木英彦、李相儒、近藤昌夫、渡利彰浩、八木清仁
  • Organizer: 第32回分子病理研究会
  • Place of Presentation: 奈良
• [Presentation] Claudin-3 and -4 binderの体内動態解析および安全性評価 (2013)
  • Author(s): 李相儒、近藤昌夫、渡利彰浩、八木清仁
  • Organizer: 第32回分子病理研究会
  • Place of Presentation: 奈良
• [Presentation] Claudinを標的とした粘膜ワクチン技術の開発 (2013)
  • Author(s): 土山遼、鈴木英彦、角谷英樹、渡利彰浩、近藤昌夫、八木清仁
  • Organizer: 第32回分子病理研究会
  • Place of Presentation: 奈良
• [Presentation] Claudin-3/-4結合分子(C-CPE)を利用する体内動態および安全性解析 (2013)
  • Author(s): 李相儒、近藤昌夫、渡利彰浩、八木清仁
  • Organizer: 日本薬物動態学会 第28年会
  • Place of Presentation: 東京
• [Presentation] 抗Claudin-1抗体の創製と評価 (2013)
  • Author(s): 山下真代、長瀬翔太郎、飯田愛未、白砂圭崇、深澤征義、近藤昌夫、多田 稔、石井明子、渡利彰浩、八木清仁
  • Organizer: 日本薬物動態学会 第28年会
  • Place of Presentation: 東京
• [Presentation] 上皮細胞を標的とした創薬基盤研究 第1報～Claudin binderのバアリ制御機構の解析～ (2013)
  • Author(s): 土山遼、Susanne M Krug、Michael From、近藤昌夫、八木清仁
  • Organizer: 日本薬学会 第133年会
  • Place of Presentation: 横浜市
• [Presentation] 上皮細胞を標的とした創薬基盤研究 第2報～免疫抗体ライブラリの最適化～ (2013)
  • Author(s): 山下真代、長瀬翔太郎、高橋梓、岩成宏子、近藤昌夫、渡利彰浩、浜窪隆雄、八木清仁
  • Organizer: 日本薬学会 第133年会
  • Place of Presentation: 横浜市
• [Presentation] 上皮細胞を標的とした創薬基盤研究 第3報～Claudin-1特異的binderの創製～ (2013)
  • Author(s): 長瀬翔太郎、山下真代、飯田愛未、渡利彰浩、近藤昌夫、深潭征義, 八木清仁
  • Organizer: 日本薬学会 第133年会
  • Place of Presentation: 横浜市
• [Presentation] 上皮細胞を標的とした創薬基盤研究 第4報～Claudin-binder-4特異的binderの創製～ (2013)
  • Author(s): 平垣有史、李相儒、清水芳実、渡利彰浩、近藤昌夫、深潭征義、八木清仁
  • Organizer: 日本薬学会 第133年会
  • Place of Presentation: 横浜市
• [Presentation] 上皮細胞を標的とした創薬基盤研究 第5報～低分子tight junction modulator の創製～ (2013)
  • Author(s): 長谷川真希、渡利彰浩、近藤昌夫、八木清仁
  • Organizer: 日本薬学会 第133年会
  • Place of Presentation: 横浜市
• [Presentation] 上皮細胞を標的とした創薬基盤研究 第6報～Tricellular junction modulatorの創製～ (2013)
  • Author(s): 早石知浩、近藤昌夫、渡利彰浩、永浜正博、八木清仁
  • Organizer: 日本薬学会 第133年会
  • Place of Presentation: 横浜市
• [Presentation] 上皮細胞を標的とした創薬基盤研究 第7報～Claudin-3/-4結合分子(C-CPE)の体内動態解析～ (2013)
  • Author(s): 李相儒、近藤昌夫、渡利彰浩、八木清仁
  • Organizer: 日本薬学会 第133年会
  • Place of Presentation: 横浜市
• [Presentation] タイトジャンクションによる薬物代謝活性制御の可能性 (2013)
  • Author(s): 清水芳実、近藤昌夫、渡利彰浩、深潭征義、八木清仁
  • Organizer: 日本薬学会 第133年会
  • Place of Presentation: 横浜市
• [Presentation] Claudinを標的とした動態制御技術の現状と課題 (2012)
  • Author(s): 近藤昌夫、八木清仁
  • Organizer: 創剤フォーラム第18回若手研究会講演
  • Place of Presentation: 徳島市(招待講演)
  • Year and Date: 2012-11-17
• [Presentation] タイトジャンクションを標的とした粘膜吸収促進技術の螺旋的発展 (2012)
  • Author(s): 近藤昌夫、八木清仁
  • Organizer: 第34生体膜と薬物の相互作用シンポジウム
  • Place of Presentation: 京都市(招待講演)
  • Year and Date: 2012-11-16
• [Presentation] Spiral progression in epithelium-target ed drug development (2012)
  • Author(s): Masuo Kondoh, Kiyohito Yagi
  • Organizer: International Symposium on Epithelial Barrier and Transport 2012
  • Place of Presentation: 草津市(招待講演)
  • Year and Date: 2012-09-15
• [Presentation] 高親和性claudin-4 binderの創製と粘膜ワクチンへの応用 (2012)
  • Author(s): 鈴木英彦、山根誠司、角谷英樹、高橋梓、内田博司、渡利彰浩、近藤昌夫、八木清仁
  • Organizer: 第28回日本DDS学会学術集会
  • Place of Presentation: 札幌市
  • Year and Date: 2012-07-04
• [Presentation] Claudinを標的とした動態制御技術の現状と課題 (2012)
  • Author(s): 近藤昌夫、八木清仁
  • Organizer: 第28回日本DDS学会学術集会
  • Place of Presentation: 札幌市(招待講演)
  • Year and Date: 2012-07-04
• [Presentation] Preparation of a broad-specific claudin binder by using Clotridium perfringcns enterotoxin. (2012)
  • Author(s): Hidehiko Suzuki, Azusa Takahashi, Koji Mat sushita, Xiangru Li, Hiroshi Tsujino, Akihiro Watari, Masuo Kondoh, Hiroshi Aoyama, Tadayuki Uno, Kiyohito Yagi
  • Organizer: Experimental Biology 2012
  • Place of Presentation: San Diego, USA
• [Presentation] Clostridium perfringens enterotoxin 断片を利用したclaudin指向性吸収促進技術の安全性評価 (2012)
  • Author(s): 土山遼、長瀬翔太郎、鈴木英彦、李相儒、山根誠司、渡利彰浩、近藤昌夫、八木清仁
  • Organizer: 第59回トキシンシンポジウム
  • Place of Presentation: 帯広市
• [Presentation] Establishment of a cell-based screening system for chemical modulators of claudin expression (2012)
  • Author(s): Akihiro Watari, Maki Hasegawa, Masuo Kondoh, Kiyohito Yagi
  • Organizer: Tight junction conference 2012
  • Place of Presentation: Merida, MEXICO
• [Presentation] Biochemical analysis of a claudin ligand that exhibits a broad binding specificity (2012)
  • Author(s): Ryo Doyama, Hidehiko Suzuki, Kyohei Matsushita、Xiangru Li, Azusa Takahashi, Koji Matsuhisa, Hiorshi Tsujino, Akihiro Watari、Masuo Kondoh, Hiroshi Aoyama, Tadayuki Uno, Kiyohito Yagi
  • Organizer: Tight junction conference 2012
  • Place of Presentation: Merida, MEXICO
• [Presentation] Structual and biochemical analysis of a novel, broadly specific claudin binder (2012)
  • Author(s): Shotaio Nagase, Koj i Matsushita, Azusa Takahashi, Hiroshi Tsujino, Xiangru Li, Hidehiko Suzuki, Hiroshi. Aoyama, Masuo Kondoh, Akihiro Watari, Tadayuki Uno, Takao Hamakubo, Kiyohito Yagi
  • Organizer: 52nd Annual meeting of the American society for Ce11 Biology
  • Place of Presentation: San Francisco, USA
• [Presentation] Creation of a novel claudin binder by using Clostridium perfringens enterotoxin and baculoviral display. (2012)
  • Author(s): Azusa Takahashi, Yumiko Saito, Masuo Kondoh, Hideki Kakutani, Hidehiko Suzuki, Koji Matsuhisa, Akihiro Watari, Takao Hamakubo, Kiyohito Yagi
  • Organizer: 52nd Annual meeting of the American society for Ce11 Biology
  • Place of Presentation: San Francisco, USA
• [Presentation] 第29回日本DDS学会
  • Author(s): 近藤昌夫、八木清仁
  • Organizer: 第66回日本細菌学会中国・四国支部総会
  • Place of Presentation: 広島
  • Invited
• [Patent(Industrial Property Rights)] 抗体、フラグメント、医薬組成物、分子及び腫瘍マーカー (2014)
  • Inventor(s): 近藤昌夫、國安弘基、深澤征義、石井明子、多田稔、八木清仁
  • Industrial Property Rights Holder: 近藤昌夫、國安弘基、深澤征義、石井明子、多田稔、八木清仁
  • Industrial Property Rights Type: 特許
  • Filing Date: 2014-02-27
  • Overseas
• [Patent(Industrial Property Rights)] 抗体、フラグメント及び抗HCV治療剤 (2013)
  • Inventor(s): 近藤昌夫、深澤征義、石井明子、多田稔、八木清仁、渡利彰浩
  • Industrial Property Rights Holder: 近藤昌夫、深澤征義、石井明子、多田稔、八木清仁、渡利彰浩
  • Industrial Property Rights Type: 特許
  • Filing Date: 2013-11-08
  • Overseas
• [Patent(Industrial Property Rights)] 抗体、フラグメント及び抗HCV治療剤 (2013)
  • Inventor(s): 近藤昌夫、深津征義、石井明子、多田稔、八木清仁、渡利彰浩
  • Industrial Property Rights Holder: ヒューマンサイエンス振興財団、大阪大学
  • Industrial Property Number: 2013-038059
  • Filing Date: 2013-02-28
• [Patent(Industrial Property Rights)] 抗体、フラグメント、医薬組成物、分子及び腫瘍マーカー (2013)
  • Inventor(s): 近藤昌夫、國安弘基、深洋征義、石井明子、多田稔、八木清仁
  • Industrial Property Rights Holder: ヒューマンサイエンス振興財団、奈良県立医科大学
  • Industrial Property Number: 2013-040211
  • Filing Date: 2013-03-01