| Budget Amount *help |
¥18,720,000 (Direct Cost: ¥14,400,000、Indirect Cost: ¥4,320,000)
Fiscal Year 2014: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2013: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2012: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
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| Outline of Final Research Achievements |
We published papers containing analyses of Rab8a,b, PKD1,2, and FAPP2 knockout mice. We finished analysis of pancreas-specific SNAP23 knockout mice and identified molecular mechanism for increased insulin secretion in these mice. The results were summarized and submitted to a journal. We are analyzing the molecular mechanisms underlining phenotypes of syntaxin3 knockout mice by generating KO cell lines by CRISPR/Cas9 method. For identifying molecular mechanism of apical transport, we discovered a Rab8a binding protein and its role for apical transport. For monitoring the transport of apical proteins, we generated a model protein and introduced it into cultured intestinal organoids. We found out novel candidate molecules for apical transport using C. elegans. We generated knockout mice lacking these molecules and analyzing their phenotypes.
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