• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Neural mechanisms underlying the circadian pacemakers

Research Project

Project/Area Number 24390052
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field General physiology
Research InstitutionKanazawa University

Principal Investigator

Mieda Michihiro  金沢大学, 医学系, 准教授 (20296552)

Co-Investigator(Renkei-kenkyūsha) SAKURAI TAKESHI  金沢大学, 医学系, 教授 (60251055)
Project Period (FY) 2012-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥18,850,000 (Direct Cost: ¥14,500,000、Indirect Cost: ¥4,350,000)
Fiscal Year 2015: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2014: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2013: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2012: ¥7,930,000 (Direct Cost: ¥6,100,000、Indirect Cost: ¥1,830,000)
Keywords概日リズム / 視交叉上核 / バソプレシン / 遺伝子操作マウス / 体内時計 / 神経ネットワーク / 時計遺伝子 / トランスジェニックマウス / Bmal1 / VIP / casein kinase 1d / Vgat / SCN / AVP / 食餌
Outline of Final Research Achievements

The suprachiasmatic nucleus (SCN), the primary circadian pacemaker in mammals, is a network structure composed of multiple types of neurons. However, the neural mechanisms underlying the generation of robust and stable circadian oscillation by the SCN network remains unknown. We generated mice with neuron type-specific genetic manipulations and performed analyses of their behavior, gene expression in vivo and ex vivo, and so on. We found that AVP producing neurons in the SCN play a critical role in the generation of stable circadian behavioral rhythms and the determination of the circadian period.

Report

(5 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Annual Research Report
  • 2013 Annual Research Report
  • 2012 Annual Research Report
  • Research Products

    (37 results)

All 2016 2015 2014 2013 2012 Other

All Journal Article (13 results) (of which Peer Reviewed: 10 results,  Open Access: 2 results,  Acknowledgement Compliant: 1 results) Presentation (21 results) (of which Int'l Joint Research: 2 results,  Invited: 8 results) Book (3 results)

  • [Journal Article] Genetic dissection of neural mechanisms underlying the central circadian pacemaker2016

    • Author(s)
      Mieda M, Ono D, Hasegawa E, Okamoto H, Honma K, Honma S, Sakurai T.
    • Journal Title

      The proceeding volume of Sapporo Symposium on Biological Rhythm in 2014

      Volume: in press Pages: 139-148

    • Related Report
      2015 Annual Research Report
  • [Journal Article] 睡眠障害とオレキシン拮抗薬2016

    • Author(s)
      三枝理博,櫻井武
    • Journal Title

      Annual Review 糖尿病・代謝・内分泌2016

      Volume: NA Pages: 197-205

    • Related Report
      2015 Annual Research Report
  • [Journal Article] 概日時計による睡眠の調節2015

    • Author(s)
      三枝理博
    • Journal Title

      実験医学

      Volume: 33 Pages: 2071-2076

    • Related Report
      2015 Annual Research Report
  • [Journal Article] Cellular Clocks in AVP Neurons of the SCN Are Critical for Interneuronal Coupling Regulating Circadian Behavior Rhythm.2015

    • Author(s)
      Mieda M, Ono D, Hasegawa E, Okamoto H, Honma K, Honma S, Sakurai T.
    • Journal Title

      Neuron

      Volume: 85 Issue: 5 Pages: 1103-1116

    • DOI

      10.1016/j.neuron.2015.02.005

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Orexin neurons suppress narcolepsy via 2 distinct efferent pa thways.2014

    • Author(s)
      Hasegawa E, Yanagisawa M, Sakurai T, Mieda M.
    • Journal Title

      J Clin Invest.

      Volume: 124 Issue: 2 Pages: 604-16

    • DOI

      10.1172/jci71017

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] GABAergic neurons in the preoptic area send direct inhibitory projections to orexin neurons.2013

    • Author(s)
      Saito YC, Tsujino N, Hasegawa E, Akashi K, Abe M, Mieda M, Sakimura K, Sakurai T.
    • Journal Title

      Front Neural Circuits.

      Volume: 7 Pages: 192-192

    • DOI

      10.3389/fncir.2013.00192

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Orexin receptor-1 in the locus coeruleus plays an important role in cue- dependent fear memory consolidation.2013

    • Author(s)
      Soya S, Shoji H, Hasegawa E, Hondo M, Miyakawa T, Yanagisawa M, Mieda M, Sakurai T.
    • Journal Title

      J Neurosci.

      Volume: 33 Issue: 36 Pages: 14549-57

    • DOI

      10.1523/jneurosci.1130-13.2013

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Neurotensin co-expressed in orexin-producing neurons in the lateral hypothalamus plays an important role in regulation of sleep/wakefulness States.2013

    • Author(s)
      Furutani N, Hondo M, Kageyama H, Tsujino N, Mieda M, Yanagisawa M, Shioda S, Sakurai T.
    • Journal Title

      PLoS One

      Volume: 8 Issue: 4 Pages: e62391-e62391

    • DOI

      10.1371/journal.pone.0062391

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Differential roles of orexin receptors in the regulation of sleep/wakefulness2013

    • Author(s)
      Mieda M, Tsujino N, Sakurai T.
    • Journal Title

      Front Endocrinol

      Volume: 4 Pages: 57-57

    • DOI

      10.3389/fendo.2013.00057

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Neurotensin co-expressed in orexin-producing neurons in the lateral hypothalamus plays an important role in regulation of sleep/wakefulness states2013

    • Author(s)
      Furutani. N
    • Journal Title

      PLoS one

      Volume: (in press)

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Orexin (hypocretin) receptor agonists and antagonists for treatment of sleep disorders. Rationale for development and current status.2013

    • Author(s)
      Mieda. M
    • Journal Title

      CNS Drugs

      Volume: 27 Issue: 2 Pages: 83-90

    • DOI

      10.1007/s40263-012-0036-8

    • NAID

      120005254048

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Clock genes influence gene expression in growth plate and endochondral ossification in mice.2012

    • Author(s)
      Takarada. T
    • Journal Title

      J Biol Chem

      Volume: 287 Pages: 36081-36095

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Overview of orexin/hypocretin system.2012

    • Author(s)
      Mieda. M
    • Journal Title

      Prog Brain Res

      Volume: 198 Pages: 5-14

    • DOI

      10.1016/b978-0-444-59489-1.00002-1

    • NAID

      120004419378

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Presentation] 中枢概日ペースメーカーにおけるバソプレシン産生ニューロンの役割2016

    • Author(s)
      三枝理博
    • Organizer
      日本解剖学会
    • Place of Presentation
      ビッグパレットふくしま(福島県郡山市)
    • Year and Date
      2016-03-28
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] ニューロンタイプ特異的遺伝子操作による中枢概日ペースメーカー神経機構の解析2016

    • Author(s)
      三枝理博
    • Organizer
      日本生理学会
    • Place of Presentation
      札幌コンベンションセンター(北海道札幌市)
    • Year and Date
      2016-03-22
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] 中枢概日時計におけるバソプレシン産生ニューロンの役割2015

    • Author(s)
      三枝理博
    • Organizer
      日本分子生物学会年会・日本生化学会大会 合同大会
    • Place of Presentation
      神戸コンベンションセンター(兵庫県神戸市)
    • Year and Date
      2015-12-01
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] Cellular clocks in AVP neurons of the SCN are critical for interneuronal coupling regulating circadian behavior rhythm2015

    • Author(s)
      Mieda M.
    • Organizer
      Asian Forum on Chronobiology in 2015
    • Place of Presentation
      北海道大学(北海道札幌市)
    • Year and Date
      2015-09-07
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Cellular clocks in AVP neurons of the SCN are critical for interneuronal coupling regulating circadian behavior rhythm2015

    • Author(s)
      Mieda M.
    • Organizer
      European Biological Rhythms Society
    • Place of Presentation
      University of Manchester (Manchester, UK)
    • Year and Date
      2015-08-02
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Cellular Circadian Oscillators in Vasopressin Neurons of the Suprachiasmatic Nucleus Play a Critical Role in Coupling between Morning and Evening Behavioral Rhythms in Mice2015

    • Author(s)
      Mieda, M.
    • Organizer
      日本生理学会
    • Place of Presentation
      神戸コンベンションセンター(兵庫県神戸市)
    • Year and Date
      2015-03-22 – 2015-03-23
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Roles of AVP-producing neurons in the central circadian pacemaker of the suprachiasmatic nucleus.2014

    • Author(s)
      Mieda, M.
    • Organizer
      日本神経科学会
    • Place of Presentation
      パシフィコ横浜(神奈川県横浜市)
    • Year and Date
      2014-09-11 – 2014-09-13
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Genetic dissection of neural mechanisms underlying the central circadian pacemaker2014

    • Author(s)
      Mieda, M.
    • Organizer
      Sapporo Symposium on Biological Rhythm
    • Place of Presentation
      北海道大学(北海道札幌市)
    • Year and Date
      2014-07-25 – 2014-07-26
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Roles of AVP-producing neurons in the central circadian pacemaker of the suprachiasmatic nucleus2014

    • Author(s)
      Mieda M, Ono D, Hasegawa E, Okamoto H, Honma K, Honma S, Sakurai T.
    • Organizer
      Society for Reseach on Biological Rhythms
    • Place of Presentation
      Conference Center at Big Sky Resort (MT, USA)
    • Year and Date
      2014-06-14 – 2014-06-18
    • Related Report
      2014 Annual Research Report
  • [Presentation] Roles of AVP-producing neurons in the central circadian pacemaker of the suprachiasmatic nucleus2014

    • Author(s)
      Mieda, M. Ono, D. Hasegawa, E. Okamoto, H. Honma, S. Sakurai, T.
    • Organizer
      Gordon Research Conferences, Sleep Regulation & Function,
    • Place of Presentation
      Hotel Galvez (USA)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Roles of AVP-producing neurons in the central circa dian pacemaker of the suprachiasmatic nucleus2013

    • Author(s)
      三枝理博
    • Organizer
      第90回生理学会
    • Place of Presentation
      タワーホール船堀(東京都)
    • Year and Date
      2013-03-29
    • Related Report
      2012 Annual Research Report
  • [Presentation] Roles of AVP-producing neurons in the central circadian pacemaker of the suprachiasmatic nucleus.2013

    • Author(s)
      三枝理博
    • Organizer
      脳・肝インターフェースメディシン研究センターシンポジウム
    • Place of Presentation
      東京国際フォーラム(東京都)(招待講演)
    • Year and Date
      2013-03-26
    • Related Report
      2012 Annual Research Report
  • [Presentation] 視交叉上核AVP産生ニューロンは概日周期・活動時間の決定に重要な役割を果たす2013

    • Author(s)
      三枝理博、小野大輔、長谷川恵美、岡本仁、本間さと、櫻井武
    • Organizer
      第20回日本時間生物学会学術大会
    • Place of Presentation
      近畿大学東大阪キャンパス(大阪府)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Dissection of neural mechanisms underlying circadian pacemakers usingbrain region/cell-specific Bmall deficient mice.2012

    • Author(s)
      Mieda. M
    • Organizer
      The 7th Asian Sleep Research Society Congress
    • Place of Presentation
      Taipei International Convention Center (Taiwan)(招待講演)
    • Year and Date
      2012-12-02
    • Related Report
      2012 Annual Research Report
  • [Presentation] 脳領域・細胞特異的Bmall欠損マウスを用いた概日ペースメーカー機構の解析2012

    • Author(s)
      三枝理博
    • Organizer
      日本睡眠学会第37回定期学術集会
    • Place of Presentation
      パシフィコ横浜(神奈川県)(招待講演)
    • Year and Date
      2012-10-28
    • Related Report
      2012 Annual Research Report
  • [Presentation] Differential regulation of non-REM and REM sleep by orexinergic neurons2012

    • Author(s)
      Mieda. M
    • Organizer
      Neuroscience 2012
    • Place of Presentation
      Morial Convention Center (USA)(招待講演)
    • Year and Date
      2012-10-17
    • Related Report
      2012 Annual Research Report
  • [Presentation] Neural mechanisms underlying food-entrainable circadian rhythms2012

    • Author(s)
      Mieda. M
    • Organizer
      日本味と匂学会 第46回大会
    • Place of Presentation
      大阪大学(大阪府)(招待講演)
    • Year and Date
      2012-10-03
    • Related Report
      2012 Annual Research Report
  • [Presentation] 概日ペースメーカー・視交叉上核神経ネットワークにおけるAVP産生ニューロンの役割2012

    • Author(s)
      三枝理博
    • Organizer
      時間生物学会
    • Place of Presentation
      北海道大学(北海道)
    • Year and Date
      2012-09-16
    • Related Report
      2012 Annual Research Report
  • [Presentation] Search for neurons mediating functions of orexin as a wakefulness-stabilizer2012

    • Author(s)
      Hasegawa. E
    • Organizer
      21st Congress of the European Sleep Research Society
    • Place of Presentation
      CNIT Paris La Defnse (フランス)
    • Year and Date
      2012-09-07
    • Related Report
      2012 Annual Research Report
  • [Presentation] Neural mechanisms underlying food-entrainable circadian rhythms2012

    • Author(s)
      Mieda. M
    • Organizer
      26th Annual Meeting of the Associated Professional Sleep Societies
    • Place of Presentation
      John B. Hynes Veterans Memorial Convention Center (USA)(招待講演)
    • Year and Date
      2012-06-10
    • Related Report
      2012 Annual Research Report
  • [Presentation] 概日ペースメーカー・視交叉上核神経ネットワークにおけるAVP産生ニューロンの役割

    • Author(s)
      三枝理博、長谷川恵美、岡本仁、櫻井武
    • Organizer
      第36回日本神経科学学会大会
    • Place of Presentation
      京都国際会議場(京都府)
    • Related Report
      2013 Annual Research Report
  • [Book] Orexin (Hypocretin) and Narcolepsy. In Narcolepsy, 2nd Edition: A Clinical Guide2016

    • Author(s)
      Mieda M, Sakurai T. (Goswami M, Thorpy MJ, Pandi-Perumal SR, eds)
    • Publisher
      Springer
    • Related Report
      2015 Annual Research Report
  • [Book] Pharmacogenetic dissection of neural mechanisms underlying the regulation of sleep/wakefulness using DREADDs. In Designer Receptors Exclusively Activated by Designer Drugs2015

    • Author(s)
      Mieda M, Sakurai T. (Thiel G. eds)
    • Publisher
      Humana Press
    • Related Report
      2015 Annual Research Report
  • [Book] Physiological roles of orexin receptors on sleep/wakefulness regulation. In Orexin and Sleep - Molecular, Functional and Clinical Aspects2015

    • Author(s)
      Mieda M, Sakurai T. (Sakurai T, Monti JM, Pandi-Perumal SR, eds)
    • Publisher
      Springer
    • Related Report
      2015 Annual Research Report

URL: 

Published: 2012-04-24   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi