Investigation of acquisition mechanism of hyper virulence by spontaneous mutation mechanism of group A streptococcus using next generation sequencers.
| Project/Area Number |
24390109
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Bacteriology (including Mycology)
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| Research Institution | Research Institute, International Medical Center of Japan |
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Principal Investigator |
MIYOSHI-AKIYAMA Tohru 独立行政法人国立国際医療研究センター, その他部局等, その他 (20246466)
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| Co-Investigator(Kenkyū-buntansha) |
KIRIKAE Teruo 国立研究開発法人, 国立国際医療研究センター (50192563)
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| Co-Investigator(Renkei-kenkyūsha) |
WATANABE Shinya 国立研究開発法人, 国立国際医療研究センター (60614956)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥18,590,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥4,290,000)
Fiscal Year 2014: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2013: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2012: ¥9,230,000 (Direct Cost: ¥7,100,000、Indirect Cost: ¥2,130,000)
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| Keywords | 次世代シーケンサー / レンサ球菌 / マウスモデル / 自発的変異 / 劇症型感染症 / RNA-seq / 遺伝子破壊株 / 溶血毒素 / 次世代シーケンサ |
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| Outline of Final Research Achievements |
Ten genes related to in vivo spontaneous mutation of group A streptococcus were deleted individually, and the mutants were tested for in vivo mutation spectral by using a mouse infection model. Deletion sdn1 or csn1 resulted in reduction of covS mutation rate.
After inoculation in mouse, gene expression profile of SDSE was analyzed comprehensively. The results showed that expression of virulence factors such as hemolysins, and enzymes degrading sugars were elevated. SDSE clinical isolates harboring st6297-type emm gene, which is prevalent type in Japanese patients, produced significantly higher amount of hemolysins in comparison with isolates harboring other types of the emm genes.
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Report
(4 results)
Research Products
(14 results)
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[Journal Article] Development of duplex PCR assay for detection and differentiation of typical and atypical Melissococcus plutonius strains.2014
Author(s)
Rie Arai, Tohru Miyoshi-Akiyama, Kayo Okumura, Yuiko Morinaga, Meihua Wu, Yuya Sugimura, Mikio Yoshiyama, Masatoshi Okura, Teruo Kirikae, Daisuke Takamatsu
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Journal Title
J. Vet. Med. Sci.
Volume: 76
Issue: 4
Pages: 491-498
DOI
NAID
ISSN
0916-7250, 1347-7439
Related Report
Peer Reviewed / Open Access
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