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Investigation of acquisition mechanism of hyper virulence by spontaneous mutation mechanism of group A streptococcus using next generation sequencers.

Research Project

Project/Area Number 24390109
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Bacteriology (including Mycology)
Research InstitutionResearch Institute, International Medical Center of Japan

Principal Investigator

MIYOSHI-AKIYAMA Tohru  独立行政法人国立国際医療研究センター, その他部局等, その他 (20246466)

Co-Investigator(Kenkyū-buntansha) KIRIKAE Teruo  国立研究開発法人, 国立国際医療研究センター (50192563)
Co-Investigator(Renkei-kenkyūsha) WATANABE Shinya  国立研究開発法人, 国立国際医療研究センター (60614956)
Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥18,590,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥4,290,000)
Fiscal Year 2014: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2013: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2012: ¥9,230,000 (Direct Cost: ¥7,100,000、Indirect Cost: ¥2,130,000)
Keywords次世代シーケンサー / レンサ球菌 / マウスモデル / 自発的変異 / 劇症型感染症 / RNA-seq / 遺伝子破壊株 / 溶血毒素 / 次世代シーケンサ
Outline of Final Research Achievements

Ten genes related to in vivo spontaneous mutation of group A streptococcus were deleted individually, and the mutants were tested for in vivo mutation spectral by using a mouse infection model. Deletion sdn1 or csn1 resulted in reduction of covS mutation rate.
After inoculation in mouse, gene expression profile of SDSE was analyzed comprehensively. The results showed that expression of virulence factors such as hemolysins, and enzymes degrading sugars were elevated. SDSE clinical isolates harboring st6297-type emm gene, which is prevalent type in Japanese patients, produced significantly higher amount of hemolysins in comparison with isolates harboring other types of the emm genes.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Annual Research Report
  • 2012 Annual Research Report
  • Research Products

    (14 results)

All 2015 2014 2013 2012

All Journal Article (6 results) (of which Peer Reviewed: 6 results,  Open Access: 1 results) Presentation (8 results)

  • [Journal Article] Development of Duplex PCR Assay for Detection and Differentiation of Typical and Atypical <i>Melissococcus plutonius</i> strains2014

    • Author(s)
      Rie Arai, Tohru Miyoshi-Akiyama, Kayo Okumura, Yuiko Morinaga, Meihua Wu, Yuya Sugimura, Mikio Yoshiyama, Masatoshi Okura, Teruo Kirikae, Daisuke Takamatsu
    • Journal Title

      Journal of Veterinary Medical Science

      Volume: 76 Issue: 4 Pages: 491-498

    • DOI

      10.1292/jvms.13-0386

    • NAID

      130003382401

    • ISSN
      0916-7250, 1347-7439
    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Complete genome sequence of Streptococcus dysgalactiae subsp. equisimilis 167 carrying Lancefield group C antigen and comparative genomics of S. dysgalactiae subsp. equisimilis strains.2013

    • Author(s)
      Watanabe S, Kirikae T, Miyoshi-Akiyama T.
    • Journal Title

      Genome Biol Evol.

      Volume: 5(9) Issue: 9 Pages: 1644-1651

    • DOI

      10.1093/infdis/jit353

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Identification of mutations involved in the requirement of potassium for growth of typical Melissococcus plutonius strains.2013

    • Author(s)
      Takamatsu D, Arai R, Miyoshi-Akiyama T, Okumura K, Okura M, Kirikae T, Kojima A, Osaki M.
    • Journal Title

      Appl Environ Microbiol.

      Volume: 79(12 Pages: 3882-3886

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Complete genome sequence of Streptococcus pyogenes M1 476, isolated from a patient with streptococcal toxic shock syndrome.2012

    • Author(s)
      Miyoshi-Akiyama T, Watanabe S, Kirikae T.
    • Journal Title

      J Bacteriol.

      Volume: 194(19) Issue: 19 Pages: 5466-5466

    • DOI

      10.1128/jb.01265-12

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Evolutionary paths of streptococcal and staphylococcal superantigens.2012

    • Author(s)
      Okumura K, Shimomura Y, Murayama SY, Yagi J, Ubukata K, Kirikae T, Miyoshi-Akiyama T.
    • Journal Title

      BMC Genomics.

      Volume: 13 Pages: 1-16

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Complete Genome Sequence of Melissococcus plutonius DAT561, a Strain That Shows an Unusual Growth Profile and Is Representative of an Endemic Cluster in Japan2012

    • Author(s)
      Okumura K, Arai R, Okura M, Kirikae T, Takamatsu D, Osaki M, Miyoshi-Akiyama T.
    • Journal Title

      J Bacteriol

      Volume: 194(11) Issue: 11 Pages: 3014-3014

    • DOI

      10.1128/jb.00437-12

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Presentation] Regulatory Network Analysis of Group A Streptococci during Sevier Invasive Diseases2015

    • Author(s)
      渡邊 真弥,切替 照雄,秋山 徹
    • Organizer
      第88回日本細菌学会総会
    • Place of Presentation
      岐阜
    • Year and Date
      2015-03-27 – 2015-03-29
    • Related Report
      2014 Annual Research Report
  • [Presentation] . The Transcriptional Responses of Group G Streptococci to Various Stress Conditions.2014

    • Author(s)
      Watanabe, S., Kirikae, T., and Miyoshi-Akiyama, T
    • Organizer
      114th General Meeting of American Society for Microbiology
    • Place of Presentation
      Boston, Massachusetts, USA
    • Year and Date
      2014-05-17 – 2014-05-20
    • Related Report
      2014 Annual Research Report
  • [Presentation] G群レンサ球菌による劇症型レンサ球菌感染症発症機構の解明2013

    • Author(s)
      渡邊真弥,切替照雄,秋山徹
    • Organizer
      第7回細菌学若手コロッセウム
    • Place of Presentation
      広島県三原市
    • Related Report
      2013 Annual Research Report
  • [Presentation] マウス感染モデルを用いたG群レンサ球菌の二成分制御因子CsrRSの解析2013

    • Author(s)
      渡邊真弥,切替照雄,秋山徹
    • Organizer
      第22回Lancefieldレンサ球菌研究会
    • Place of Presentation
      東京
    • Related Report
      2013 Annual Research Report
  • [Presentation] Transcriptomic profiling of group G Streptococci using a murine infection model.2013

    • Author(s)
      Watanabe S, Kirikae T, Miyoshi-Akiyama T.
    • Organizer
      113th General Meeting of American Society for Microbiology
    • Place of Presentation
      Denver, Colorado, USA
    • Related Report
      2013 Annual Research Report
  • [Presentation] Microarray Analysis of the Host Factors in Streptococcal Toxic Shock Syndrome in a Mouse Model2012

    • Author(s)
      T. Miyoshi-Akiyama, K. Ubukata, T. Kirikae
    • Organizer
      ASM2012 General Meeting
    • Place of Presentation
      San Francisco, California, USA
    • Related Report
      2012 Annual Research Report
  • [Presentation] マウスモデルを用いた劇症型レンサ球菌感染症における宿主応答の解析2012

    • Author(s)
      安藤(北尾)公英、奥村香世、切替照雄1)、生方公子、秋山徹.
    • Organizer
      第21回Lancefieldレンサ球菌研究会・第44回レンサ球菌感染症研究会
    • Place of Presentation
      大阪
    • Related Report
      2012 Annual Research Report
  • [Presentation] マイクロアレイを用いた劇症型感染症モデルにおけるG群レンサ球菌の発現解析2012

    • Author(s)
      渡邊真弥、切替照雄、秋山徹
    • Organizer
      第21回Lancefieldレンサ球菌研究会・第44回レンサ球菌感染症研究会
    • Place of Presentation
      大阪
    • Related Report
      2012 Annual Research Report

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Published: 2012-04-24   Modified: 2019-07-29  

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