Identification of cis-acting element for packaging of hepatitis C virus genome
| Project/Area Number |
24390111
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Partial Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Virology
|
|---|
| Research Institution | Hamamatsu University School of Medicine |
|---|
Principal Investigator |
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
SUZUKI Ryosuke 国立感染症研究所, ウイルス第二部, 主任研究官 (50342902)
ITO Masahiko 浜松医科大学, 医学部感染症学講座, 助教 (50385423)
|
|---|
| Project Period (FY) |
2012-04-01 – 2015-03-31
|
| Project Status |
Completed (Fiscal Year 2014)
|
| Budget Amount *help |
¥15,990,000 (Direct Cost: ¥12,300,000、Indirect Cost: ¥3,690,000)
Fiscal Year 2014: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2013: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2012: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
|
|---|
| Keywords | ウイルス / ゲノムパッケージング / 粒子形成 / C型肝炎ウイルス / 複製 / パッケージング |
|---|
| Outline of Final Research Achievements |
The mechanism(s) by which the hepatitis C virus (HCV) genome becomes encapsidated remains unknown. Deep sequencing demonstrated that a majority of particle-associated HCV RNAs are genome-sized. In cell cultures producing HCV, molecular ratios of 3’ end viral RNA to 5’ end positively correlated with infectivity of fractions and that of virion-rich fraction was the highest, suggesting selective encapsidation of genome RNA. Using trans-complementation systems, the 3’ untranslated region (UTR) of the genome was identified as a cis-acting element for RNA packaging. Mutations within the stem-loops at the 3’ end of the 3’ UTR were observed to diminish packaging efficiency. A foreign gene flanked by the 3’ UTR was encapsidated by supplying both viral NS3-NS5B proteins and Core-NS2 in trans. This study provides the first direct evidence of selective packaging of the HCV genome into the viral particles potentially through 3’ UTR-Core binding.
|
Report
(4 results)
Research Products
(30 results)
-
-
-
-
-
[Journal Article] Involvement of Hepatitis C Virus NS5A Hyperphosphorylation Mediated by Casein Kinase I-α in Infectious Virus Production.2014
Author(s)
Masaki T, Matsunaga S, Takahashi H, Nakashima K, Kimura Y, Ito M, Matsuda M, Murayama A, Kato T, Hirano H, Endo Y, Lemon SM, Wakita T, Sawasaki T, Suzuki T
-
Journal Title
J. Virol.
Volume: 88(13)
Pages: 7541-55
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
-
-
-
[Journal Article] Antiviral activity of glycyrrhizin against hepatitis C virus in vitro2013
Author(s)
Matsumoto Y, Matsuura T, Aoyagi H, Matsuda M, Hmwe SS, Date T, Watanabe N, Watashi K, Suzuki R, Ichinose S, Wake K, Suzuki T, Miyamura T, Wakita T, Aizaki H
-
Journal Title
DOI
Related Report
Peer Reviewed
-
-
[Journal Article] Hepatitis C virus NS3/4A protease inhibits complement activation by cleaving complement component 42013
Author(s)
Mawatari S, Uto H, Ido A, Nakashima K, Suzuki T, Kanmura S, Kumagai K, Oda K, Tabu K, Tamai T, Moriuchi A, Oketani M, Shimada Y, Sudoh M, Shoji I, Tsubouchi H
-
Journal Title
DOI
Related Report
Peer Reviewed
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
