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Development of self-assembling nanodevices for multimodal molecular imaging

Research Project

Project/Area Number 24390294
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Radiation science
Research InstitutionKobe Pharmaceutical University

Principal Investigator

MUKAI Takahiro  神戸薬科大学, 薬学部, 教授 (30284706)

Co-Investigator(Kenkyū-buntansha) SASAKI Hitoshi  長崎大学, 大学病院, 教授 (00170689)
Co-Investigator(Renkei-kenkyūsha) TANAKA Masafumi  神戸薬科大学, 薬学部, 講師 (40411904)
Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥18,330,000 (Direct Cost: ¥14,100,000、Indirect Cost: ¥4,230,000)
Fiscal Year 2014: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2013: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥10,530,000 (Direct Cost: ¥8,100,000、Indirect Cost: ¥2,430,000)
Keywords薬学 / 放射線 / ナノバイオ / イメージング
Outline of Final Research Achievements

We established a ternary anionic complex constructed with PAMAM G4-dendrimer modified with DTPA, polyethyleneimine (PEI), and γ-polyglutamic acid (γPGA) for molecular imaging. We prepared In-111- or fluorescent-labeled γPGA/PEI/G4-DTPA, and evaluated the effectiveness as a nuclear or optical imaging probe for sentinel lymph node (LN). These complexes had little agglutination activity with erythrocytes and no cytotoxicity. Upon administration into rat or mouse footpads intradermally, high uptake was observed in the first draining popliteal LN. Moreover, these complexes clearly visualized the popliteal LN with SPECT and in vivo fluorescent imager.
In-111- and Cy5 dye-labeled ternary complex hyaluronic acid (HA)/PEI/G4-Cy5-DTPA-111In was synthesized by electrostatic interaction. The HA complex demonstrated specific accumulation in CD44 positive tumor cells.
These results suggested that G4-DTPA/PEI/anionic polymer complexes would be useful as nanoprobes for multimodal imaging.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Annual Research Report
  • 2012 Annual Research Report
  • Research Products

    (10 results)

All 2015 2014 2013 2012

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Acknowledgement Compliant: 1 results) Presentation (9 results)

  • [Journal Article] Radiolabeled γ-polyglutamic acid complex as a nano-platform for sentinel lymph node imaging2014

    • Author(s)
      Kohei Sano, Yuriko Iwamiya, Tomoaki Kurosaki, Mikako Ogawa, Yasuhiro Magata, Hitoshi Sasaki, Takashi Ohshim, Minoru Maeda, Takahiro Mukai
    • Journal Title

      Journal of Controlled Release

      Volume: 194 Pages: 310-315

    • DOI

      10.1016/j.jconrel.2014.08.025

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] アポE由来ペプチドを基盤とする脂質ナノディスクの腫瘍細胞への集積性評価2015

    • Author(s)
      長谷川真理子, 田中将史, 向高弘
    • Organizer
      日本薬学会第135年会
    • Place of Presentation
      神戸サンボーホール, 神戸
    • Year and Date
      2015-03-26 – 2015-03-28
    • Related Report
      2014 Annual Research Report
  • [Presentation] 腎障害の軽減を目的としたリポソーム化ヨード造影剤の開発2014

    • Author(s)
      神寶圭介, 萩森政頼, 向高弘
    • Organizer
      第64回日本薬学会近畿支部大会
    • Place of Presentation
      京都薬科大学, 京都
    • Year and Date
      2014-10-11
    • Related Report
      2014 Annual Research Report
  • [Presentation] 腫瘍イメージングを目指した葉酸修飾脂質ナノディスクの細胞集積性の評価2014

    • Author(s)
      辻野奈緒美, 田中将史, 向高弘
    • Organizer
      第64回日本薬学会近畿支部大会
    • Place of Presentation
      京都薬科大学, 京都
    • Year and Date
      2014-10-11
    • Related Report
      2014 Annual Research Report
  • [Presentation] Development of fluorescent γ-polyglutamic acid complex for sentinel lymph node imaging2014

    • Author(s)
      Hagimori M., Hatabe E., Sano K., Sasaki H., Saji H., Mukai T.
    • Organizer
      2014 World Molecular Imaging Congress
    • Place of Presentation
      COEX Convention Center, Seoul
    • Year and Date
      2014-09-17 – 2014-09-20
    • Related Report
      2014 Annual Research Report
  • [Presentation] センチネルリンパ節の蛍光イメージングを目指したγ-ポリグルタミン酸被覆ナノ粒子の作製2014

    • Author(s)
      千石梓, 畑辺恵里, 萩森政頼, 佐野紘平, 佐々木均, 佐治英郎, 向高弘
    • Organizer
      第14回放射性医薬品・画像診断薬研究会
    • Place of Presentation
      京都勧業館, 京都
    • Year and Date
      2014-09-13
    • Related Report
      2014 Annual Research Report
  • [Presentation] センチネルリンパ節イメージングを目指した蛍光標識γ-ポリグルタミン酸複合体の開発2014

    • Author(s)
      萩森政頼, 畑辺恵里, 佐野紘平, 佐々木均, 佐治英郎, 向高弘
    • Organizer
      第9回日本分子イメージング学会学術集会
    • Place of Presentation
      千里ライフサイエンスセンター, 豊中
    • Year and Date
      2014-05-22 – 2014-05-23
    • Related Report
      2014 Annual Research Report
  • [Presentation] センチネルリンパ節イメージングを目指した蛍光標識γ-ポリグルタミン酸複合体の開発2014

    • Author(s)
      萩森政頼, 畑辺恵里, 佐野紘平, 佐々木均, 佐治英郎, 向高弘
    • Organizer
      第9回日本分子イメージング学会学術集会
    • Place of Presentation
      千里ライフサイエンスセンター
    • Related Report
      2013 Annual Research Report
  • [Presentation] 生体イメージングを目指したγ-ポリグルタミン酸被覆蛍光ナノ粒子の作製2013

    • Author(s)
      畑辺恵里, 萩森政頼, 佐野紘平, 佐々木均, 向高弘
    • Organizer
      第63回日本薬学会近畿支部大会
    • Place of Presentation
      同志社女子大学(京田辺キャンパス)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Sentinel lymph node imaging with a novel radiolabeled γ-polyglutamic acid complex2012

    • Author(s)
      Sano K., Iwamiya Y., Kurosaki T., Ogawa M., Magata Y., Sasaki H., Oshima T., Maeda M., Mukai T ,
    • Organizer
      The Society of Nuclear Medicine 59th Annual Meeting
    • Place of Presentation
      Miami Beach (USA)
    • Year and Date
      2012-06-10
    • Related Report
      2012 Annual Research Report

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Published: 2012-04-24   Modified: 2019-07-29  

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