• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Molecular mechanism in direct effect of VEGF onto breast cancer cells

Research Project

Project/Area Number 24390301
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field General surgery
Research InstitutionKyoto University

Principal Investigator

SATO FUMIAKI  京都大学, 医学(系)研究科(研究院), 准教授 (20467426)

Co-Investigator(Kenkyū-buntansha) TOI Masakazu  京都大学, 大学院医学研究科, 教授 (10207516)
UENO Takayuki  京都大学, 大学院医学研究科, 助教 (40452362)
SAJI Shigehira  京都大学, 大学院医学研究科, 特定准教授 (80446567)
Co-Investigator(Renkei-kenkyūsha) MIKAMI Yoshiki  京都大学, 大学院医学研究科, 准教授 (90248245)
Project Period (FY) 2012-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥18,200,000 (Direct Cost: ¥14,000,000、Indirect Cost: ¥4,200,000)
Fiscal Year 2015: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2014: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2013: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2012: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Keywords乳癌 / VEGF / NRP1 / 細胞運動 / 乳がん / microRNA / 血管新生 / bevacizumab / マイクロRNA / 血管内皮増殖因子
Outline of Final Research Achievements

The purpose of this study is to elucidate the molecular mechanism of direct effect by VEGF to breast cancer cells. In this study, VEGF signal into MB-231 cells was blocked by VEGF-knockout and soluble NRP1 expression. Experiments using these cells showed that MB-231 cells are directly stimulated by VEGF via NRP1 not VEGFR, which contributes to spindle-like morphology formation and higher cell migration activity of breast cancer cells. According to microarray-based gene expression analysis of these cells, VEGF/NRP1 signaling modulates the expression of factor X that is negative regulator of cdc42. In summary, VEGF changes morphology of MB-231 cells and stimulates motility of MB-231 cells by VEGF/NRP1/factor X/cdc42 signaling pathway.

Report

(5 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Annual Research Report
  • 2013 Annual Research Report
  • 2012 Annual Research Report
  • Research Products

    (9 results)

All 2016 2015 2014 2013 2012

All Journal Article (7 results) (of which Peer Reviewed: 2 results,  Open Access: 1 results) Presentation (2 results) (of which Int'l Joint Research: 1 results)

  • [Journal Article] An optical labeling-based proliferation assay system reveals the paracrine effect of interleukin-6 in breast cancer.2015

    • Author(s)
      Itou J, Tanaka S, Sato F, Akiyama R, Kawakami Y, Toi M.
    • Journal Title

      Biochim Biophys Acta.

      Volume: 1853 Issue: 1 Pages: 27-40

    • DOI

      10.1016/j.bbamcr.2014.10.004

    • NAID

      120005512254

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] 分子標的治療のための体細胞遺伝子検査の現況 ー乳癌ー2015

    • Author(s)
      佐藤史顕、佐治重衡、戸井雅和
    • Journal Title

      遺伝子MOOK

      Volume: 28 Pages: 81-86

    • Related Report
      2014 Annual Research Report
  • [Journal Article] Genetic tumor evolution2014

    • Author(s)
      佐藤史顕、佐治重衡、戸井雅和
    • Journal Title

      Cancer Board 乳癌

      Volume: 7 Pages: 23-30

    • Related Report
      2014 Annual Research Report
  • [Journal Article] microRNA chipによるバイオマーカー開発2014

    • Author(s)
      佐藤史顕
    • Journal Title

      がん分子標的治療

      Volume: 12 Pages: 88-94

    • Related Report
      2014 Annual Research Report
  • [Journal Article] 乳がんにおけるコンパニオン診断2014

    • Author(s)
      佐藤史顕、佐治重衡、三上芳喜
    • Journal Title

      医学のあゆみ

      Volume: 248(11) Pages: 845-850

    • Related Report
      2013 Annual Research Report
  • [Journal Article] Potential clinical applications of matrix metalloproteinase inhibitors and their future prospects.2013

    • Author(s)
      Li W, Saji S, Sato F, Noda M, Toi M
    • Journal Title

      Int J Biol Markers.

      Volume: 28(2) Issue: 2 Pages: 117-30

    • DOI

      10.5301/jbm.5000026

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] microRNAの診断への応用2012

    • Author(s)
      佐藤史顕
    • Journal Title

      日本臨牀増刊号、乳癌(第2版)

      Volume: 70 Pages: 218-226

    • Related Report
      2012 Annual Research Report
  • [Presentation] VEGFA/NRP1 signal contributes to cell adhesion and motility in breast cancer cells2016

    • Author(s)
      Kiso M, Sato F, Sunao Tanaka, Masakazu Toi
    • Organizer
      米国癌学会
    • Place of Presentation
      New Orleans
    • Year and Date
      2016-04-17
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 乳癌細胞における血管内皮増殖因子(VEGF)関連microRNAの解析2013

    • Author(s)
      木曾末厘乃、佐藤史顕、蒲風玲、佐治重衡、戸井雅和
    • Organizer
      日本分子標的治療学会
    • Place of Presentation
      京都国際会館、京都
    • Related Report
      2013 Annual Research Report

URL: 

Published: 2012-04-24   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi