Establishment of 4th generation of islet isolation and the clinical application
Project/Area Number |
24390316
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Partial Multi-year Fund |
Section | 一般 |
Research Field |
Digestive surgery
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Research Institution | University of the Ryukyus (2014) Clinical research Center, Chiba-East National Hospital, National Hospital Organization (2013) Okayama University (2012) |
Principal Investigator |
NOGUCHI Hirofumi 琉球大学, 医学(系)研究科(研究院), 教授 (50378733)
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Co-Investigator(Kenkyū-buntansha) |
SAITOH Issei 新潟大学, 医歯学系, 准教授 (90404540)
藤原 俊義 岡山大学, 医歯(薬)学総合研究科, 教授 (00304303)
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Project Period (FY) |
2012-04-01 – 2015-03-31
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Project Status |
Completed (Fiscal Year 2014)
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Budget Amount *help |
¥18,330,000 (Direct Cost: ¥14,100,000、Indirect Cost: ¥4,230,000)
Fiscal Year 2014: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2012: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000)
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Keywords | 膵島移植 / 膵島分離 / JNK抑制剤 / 新規臓器保存液 / 膵島純化 / 連続比重勾配 / 連続密度勾配 / JNK抑制ペプチド / 新規臓器保存液(HN-1溶液) / 連続比重・密度勾配による純化 / 臓器保存液 |
Outline of Final Research Achievements |
We previously established a new islet isolation protocol(3rd generation) based on the Ricordi method. It includes pancreatic ductal protection, a MK preservation solution, the two-layer method of pancreas preservation, a new islet purification solution (Iodixanol-based solution), supplemental purification, and cold preservation of isolated islets. In this study, we established 4th generation of islet isolation protocol, including HN-1 preservation solution, ductal injection with JNK inhibitor, and controll-density and osmolality solution for islet purification. Establishment of this method enables us to make diabetic patients insulin independent using islets not only from 2 or 3 pancreata of NHBDs, but also from a pancreas from a brain-dead donor.
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Report
(4 results)
Research Products
(47 results)
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[Journal Article] A new method for generating insulin-secreting cells from human pancreatic epithelial cells after islet isolation transformed by NeuroD1.2014
Author(s)
Shimoda M, Chen S, Noguchi H, Takita M, Sugimoto K, Itoh T, Chujo D, Iwahashi S, Naziruddin B, Levy MF, Matsumoto S, Grayburn PA.
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Journal Title
Hum Gene Ther Methods.
Volume: 25
Issue: 3
Pages: 206-219
DOI
Related Report
Peer Reviewed
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[Journal Article] Novel positively charged nanoparticle labeling for in vivo imaging of adipose tissue-derived stem cells.2014
Author(s)
Yukawa H, Nakagawa S, Yoshizumi Y, Watanabe M, Saito H, Miyamoto Y, Noguchi H, Oishi K, Ono K, Sawada M, Kato I, Onoshima D, Obayashi M, Hayashi Y, Kaji N, Ishikawa T, Hayashi S, Baba Y.
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Journal Title
PLoS One.
Volume: 9
Issue: 11
Pages: e110142-e110142
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] In vivo imaging of transplanted islets labeled with a novel cationic nanoparticle2013
Author(s)
Oishi K, Miyamoto Y, Saito H, Murase K, Ono K, Sawada M, Watanabe M, Noguchi Y, Fujiwara T, Hayashi S, Noguchi H
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Journal Title
PLOS ONE
Volume: 8(2)
Issue: 2
Pages: e57046-e57046
DOI
Related Report
Peer Reviewed
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[Journal Article] Cancer stem cell-like characteristics of a CD133(+) subpopulation in the J82 human bladder cancer cell line.2013
Author(s)
Huang P, Watanabe M, Kaku H, Ueki H, Noguchi H, Sugimoto M, Hirata T, Yamada H, Takei K, Zheng S, Xu K, Nasu Y, Fujii Y, Liu C, Kumon H.
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Journal Title
Mol Clin Oncol.
Volume: 1
Issue: 1
Pages: 180-184
DOI
Related Report
Peer Reviewed
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[Journal Article] Observation of positively-charged magnetic nanoparticles inside HepG2 spheroids using electron microscopy.2013
Author(s)
MiyamotoY, Koshidaka Y, Noguchi H, Oishi K, Saito H, Yukawa H, Kaji N, Ikeya T, Suzuki S, Iwata H, Baba Y, Murase K, Hayashi S.
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Journal Title
Cell Med.
Volume: 5
Issue: 2-3
Pages: 89-96
DOI
Related Report
Peer Reviewed
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[Journal Article] Comparison of new preservation solution, HN-1, and University of Wisconsin solution in pancreas preservation for porcine islet isolation2013
Author(s)
Akihiro K, Noguchi H, Kuise T, Nakatsuka A, Hirota D, Kataoka H, Kawai T, Inoue K, Imagawa N, Saitoh I, Noguchi Y, Watanabe M, Wada J, Fujiwara T
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Journal Title
Cell Medicine
Volume: 6
Issue: 1-2
Pages: 3-8
DOI
Related Report
Peer Reviewed
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[Journal Article] Comparison of incubation solutions prior to islet purification in porcine islet isolation.2013
Author(s)
Kawai T, Noguchi H, Kuise T, Nakatsuka A, Katayama A, Imagawa N, Kataoka HU, Saitoh I, Noguchi Y, Watanabe M, Fujiwara T.
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Journal Title
Cell Med.
Volume: 6
Issue: 1-2
Pages: 9-14
DOI
Related Report
Peer Reviewed
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[Journal Article] Secretory Unit of Islet Transplant Objects (SUITQ) Index Can Predict Severity of Hypoglycemic Episodes in Clinical Islet Cell Transplantation.2012
Author(s)
Takita M, Matsumoto S, Qin H, Noguchi H, Shimoda M, Chujo D, Itoh T, Sugimoto K, Onaca N, Naziruddin B, Levy MF.
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Journal Title
Cell Transplant.
Volume: 21
Issue: 1
Pages: 91-98
DOI
Related Report
Peer Reviewed
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[Journal Article] Improvement of porcine islet isolation by inhibition of trypsin activity during pancreas preservation and digestion using α-antitrypsin.2012
Author(s)
Shimoda M, Noguchi H, Fujita Y, Takita M, Ikemoto T, Chujo D, Naziruddin B, Levy MF, Kobayashi N, Grayburn PA, Matsumoto S.
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Journal Title
Cell Transplant.
Volume: 21
Issue: 2-3
Pages: 465-471
DOI
Related Report
Peer Reviewed
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[Journal Article] Evaluation of osmolality of density gradient for human islet purification.2012
Author(s)
Noguchi H, Naziruddin B, Shimoda M, Fujita Y, Chujo D, Takita M, Peng H, Sugimoto K, Itoh T, Kobayashi N, Onaca N, Levy MF, Matsumoto S.
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Journal Title
Cell Transplant.
Volume: 21
Issue: 2-3
Pages: 493-500
DOI
Related Report
Peer Reviewed
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[Journal Article] Islet purification method using large bottles effectively achieves high islet yield from pig pancreas.2012
Author(s)
Shimoda M, Noguchi H, Fujita Y, Takita M, Ikemoto T, Chujo D, Naziruddin B, Levy MF, Kobayashi N, Grayburn PA, Matsumoto S.
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Journal Title
Cell Transplant.
Volume: 21
Issue: 2-3
Pages: 501-508
DOI
Related Report
Peer Reviewed
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[Journal Article] Comparison of ulinastatin, gabexate mesilate, and nafamostat mesilate in preservation solution for islet isolation.2012
Author(s)
Noguchi H, Naziruddin B, Jackson A, Shimoda M, Fujita Y, Chujo D, Takita M, Peng H, Sugimoto K, Itoh T, Kobayashi N, Ueda M, Okitsu T, Iwanaga Y, Nagata H, Liu X, Kamiya H, Onaca N, Levy MF, Matsumoto S.
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Journal Title
Cell Transplant.
Volume: 21
Issue: 2-3
Pages: 509-516
DOI
Related Report
Peer Reviewed
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[Journal Article] Fresh islets are more effective for islet transplantation than cultured islets. Cell2012
Author(s)
Noguchi H, Naziruddin B, Jackson A, Shimoda H, Ikemoto T, Fujita Y, Chujo D, Takita M, Peng H, Sugimoto K, Itoh T, Kobayashi N, Onaca N, Levy HF, Matsumoto S.
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Journal Title
Cell Transplant.
Volume: 21
Issue: 2-3
Pages: 517-523
DOI
Related Report
Peer Reviewed
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[Journal Article] Adverse events in clinical islet transplantation : one institutional experience.2012
Author(s)
Takita M, Matsumoto S, Noguchi H, Shiuoda M, Ikemoto T, Chujo D, Tamura Y, Olsen GS, Naziruddin B, Purcell K, Onaca N, Levy MF.
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Journal Title
Cell Transplant.
Volume: 21
Issue: 2-3
Pages: 547-551
DOI
Related Report
Peer Reviewed
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[Journal Article] Improved pancreatic islet isolation outcome in autologous transplantation for chronic pancreatitis.2012
Author(s)
Naziruddin B, Matsumoto S, Noguchi H, Takita M, Shimoda M, Fujita Y, Chujo D, Tate C, Onaca N, Lamont J, Kobayashi N, Levy MF.
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Journal Title
Cell Transplant.
Volume: 21
Issue: 2-3
Pages: 553-558
DOI
Related Report
Peer Reviewed
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[Journal Article] Attenuation of murine GVHD by a tea polyphenol2012
Author(s)
Kanamune, J., Iwanaga, Y., Kina, T., Noguchi, H., Matsumura, K., Uemoto, S., Hyon, S-H.
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Journal Title
Cell Transplantation
Volume: (In press)
Issue: 5
Pages: 909-918
DOI
Related Report
Peer Reviewed
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[Presentation] 膵島移植の現況2012
Author(s)
野口洋文
Organizer
第8回信州難治性肝疾患治療研究会
Place of Presentation
長野(招待講演)
Year and Date
2012-09-28
Related Report
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