|Budget Amount *help
¥18,070,000 (Direct Cost: ¥13,900,000、Indirect Cost: ¥4,170,000)
Fiscal Year 2014: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2013: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2012: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
|Outline of Final Research Achievements
Early lung adenocarcinoma cell line with constitutive Notch2 overexpression was established. TGF-B and Smad3/4, which are related to promoting epithelial mesenchymal transition, were examined by RT-PCR. Subsequently, Six1, Slug, Snail, and Hey1, down-stream molecules of Notch2 signaling pathway, were also examined.
It is difficult for small lung adenocarcinoma, a target of this study, to be applied for next generation sequencer because of its small amount of RNA. Thus, we performed further investigation of the candidate molecules that have been identified in the previous study. GBP1 was identified as a promising molecule after validation with lung adenocarcinoma cell lines and human lung adenocarcinoma specimen. In addition, wound healing assay and migration assay demonstrated that GBP1 was involved in inducing cancer invasion. We now plan to examine the association between GBP1 expression and Notch or Ras signaling pathway and the prognosis of patients with lung adenocarcinoma.