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Mechanism of malignant transformation progress from bronchioloalveolar carcinoma to invasive adenocarcinoma

Research Project

Project/Area Number 24390329
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Thoracic surgery
Research InstitutionHiroshima University

Principal Investigator

OKADA Morihito  広島大学, 原爆放射線医科学研究所, 教授 (70446045)

Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥18,070,000 (Direct Cost: ¥13,900,000、Indirect Cost: ¥4,170,000)
Fiscal Year 2014: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2013: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2012: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
Keywords肺癌 / 腺癌 / 悪性化 / Notch2 / 肺腺癌 / 外科 / トランスレーショナルリサーチ / 肺胞上皮癌
Outline of Final Research Achievements

Early lung adenocarcinoma cell line with constitutive Notch2 overexpression was established. TGF-B and Smad3/4, which are related to promoting epithelial mesenchymal transition, were examined by RT-PCR. Subsequently, Six1, Slug, Snail, and Hey1, down-stream molecules of Notch2 signaling pathway, were also examined.
It is difficult for small lung adenocarcinoma, a target of this study, to be applied for next generation sequencer because of its small amount of RNA. Thus, we performed further investigation of the candidate molecules that have been identified in the previous study. GBP1 was identified as a promising molecule after validation with lung adenocarcinoma cell lines and human lung adenocarcinoma specimen. In addition, wound healing assay and migration assay demonstrated that GBP1 was involved in inducing cancer invasion. We now plan to examine the association between GBP1 expression and Notch or Ras signaling pathway and the prognosis of patients with lung adenocarcinoma.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Annual Research Report
  • 2012 Annual Research Report

URL: 

Published: 2012-04-23   Modified: 2019-07-29  

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