Effects of Transplanted Human Cord Blood-Mononuclear Cells on Pulmonary Hypertension in Immunodeficient Mice and Their Distribution
| Project/Area Number |
24390331
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Thoracic surgery
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| Research Institution | The University of Tokushima |
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Principal Investigator |
KITAGAWA Tetsuya 徳島大学, ヘルスバイオサイエンス研究部, 教授 (80240886)
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| Co-Investigator(Kenkyū-buntansha) |
KITAICHI Takashi 徳島大学, ヘルスバイオサイエンス研究部, 准教授 (20335813)
NAKAGAWA Ryuji 徳島大学, 大学病院, 助教 (10403685)
MAEDA Kazuhisa 独立行政法人国立病院機構四国こどもとおとなの医療センター(臨床研究部(成育)、臨, 総合周産期母子医療センター, 部長 (30294680)
SUGANO Mikio 徳島大学, 大学病院, 医員 (70563807)
NAKAYAMA Taisuke 徳島大学, ヘルスバイオサイエンス研究部, 特別研究員 (80582791)
KUROBE Hirotsugu 徳島大学, ヘルスバイオサイエンス研究部, 助教 (30380083)
KINOSHITA Hajime 徳島大学, 大学病院, 特任助教 (80611227)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥18,330,000 (Direct Cost: ¥14,100,000、Indirect Cost: ¥4,230,000)
Fiscal Year 2014: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2012: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
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| Keywords | 臍帯血 / 細胞移植 / 未熟肺血管床 |
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| Outline of Final Research Achievements |
Objectives: To investigate the effects of human umbilical cord blood-derived mononuclear cell (hUCB-MNC) transplantation on pulmonary hypertension (PH) induced by monocrotaline (MCT) in immunodeficient mice and their distribution. Methods: Fresh hUCB-MNCs harvested from a human donor after her delivery were injected intravenously into those PH mice (BALB/c Slc-nu/nu) 4 weeks after MCT induction. To reveal the incorporation into the lung, CMTMR-labeled hUCB-MNCs were observed in the lung. DiR-labeled hUCB-MNCs were detected in the lung and other organs by bioimaging. Results: PH was significantly improved 4 weeks after hUCB-MNC transplantation. CMTMR-positive hUCB-MNCs were observed in the lung 3 hours after transplantation. Bioluminescence signals were detected more strongly in the lung than in other organs for 24 hours after transplantation. Conclusions: The results indicate that hUCB-MNCs are incorporated into the lung early after hUCB-MNC transplantation and improve MCT-induced PH.
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Report
(4 results)
Research Products
(39 results)
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[Journal Article] Role of macrophage-derived hypoxia-inducible factor (HIF)-1α as a mediator of vascular remodelling2013
Author(s)
Nakayama T, Kurobe H, Sugasawa N, Kinoshita H, Higashida M, Matsuoka Y, Yoshida Y, Hirata Y, Sakata M, Maxfield M, Shimabukuro M, Takahama Y, Sata M, Tamaki T, Kitagawa T, Tomita S.
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Journal Title
Cardiovasc Res.
Volume: 99
Pages: 705-715
DOI
Related Report
Peer Reviewed
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[Journal Article] Protective effects of selective mineralocorticoid receptor antagonist against aortic aneurysm progression in a novel murine model.2013
Author(s)
Kurobe H, Hirata Y, Matsuoka Y, Sugasawa N, Higashida M, Nakayama T, Maxfield MW, Yoshida Y, Shimabukuro M, Kitagawa T, Sata M.
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Journal Title
J Surg Res.
Volume: 185
Pages: 455-462
DOI
Related Report
Peer Reviewed
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[Journal Article] Beneficial effect of a synthetic prostacyclin agonist, ONO-1301, in rat autoimmune myocarditis model2013
Author(s)
Y. Hirata, H. Kurobe, E. Uematsu, S. Yagi, T. Soeki, H. Yamada, D. Fukuda, M. Shimabukuro, M. Nakayama, K. Matsumoto, Y. Sakai, T. Kitagawa, M. Sata
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Journal Title
Eur J Pharmacol.
Volume: 699
Pages: 81-87
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] Epicardial adipose tissue volume and adipocytokine imbalance are strongly linked to human coronary atherosclerosis2013
Author(s)
M. Shimabukuro, Y. Hirata, M. Tabata, M. Dagvasumberel, H. Sato, H. Kurobe, D. Fukuda, T. Soeki, T. Kitagawa, S. Takanashi, M. Sata
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Journal Title
Arterioscler Thromb Vasc Biol.
Volume: 33
Pages: 1077-1084
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] Gender disparities in the association between epicardial adipose tissue volume and coronary atherosclerosis: A 3-dimensional cardiac computed tomography imaging study in Japanese subjects2012
Author(s)
Dagvasumberel M, Shimabukuro M, Nishiuchi T, Ueno J, Takao S, Fukuda D, Hirata Y, Kurobe H, Soeki T, Iwase T, Kusunose K, Niki T, Yamaguchi K, Taketani Y, Yagi S, Tomita N, Yamada H, Wakatsuki T, Harada M, Kitagawa T, Sata M
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Journal Title
Cardiovasc Diabetol.
Volume: 11
Pages: 106-106
DOI
Related Report
Peer Reviewed
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[Presentation] Macrophage-Specific Hypoxia-Inducible Factor (HIF)-1α-Deficient Mice Suppress the Vascular Remodeling and Regulate M2 Macrophage Polarization.2013
Author(s)
Taisuke Nakayama, Hirotsugu Kurobe, Noriko Sugasawa, Hajime Kinoshita, Yasushi Yoshida, Yoichiro Hirata, Mie Sakata, Mark Webster Maxfield, Michio Shimabukuro, Yousuke Takahama, Masataka Sata, Toshiaki Tamaki, Tetsuya Kitagawa, Shuhei Tomita
Organizer
American Heart Association AHA 2013
Place of Presentation
Dallas Convention Center（アメリカ テキサス州ダラス市）
Related Report
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