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Effects of Transplanted Human Cord Blood-Mononuclear Cells on Pulmonary Hypertension in Immunodeficient Mice and Their Distribution

Research Project

Project/Area Number 24390331
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Thoracic surgery
Research InstitutionThe University of Tokushima

Principal Investigator

KITAGAWA Tetsuya  徳島大学, ヘルスバイオサイエンス研究部, 教授 (80240886)

Co-Investigator(Kenkyū-buntansha) KITAICHI Takashi  徳島大学, ヘルスバイオサイエンス研究部, 准教授 (20335813)
NAKAGAWA Ryuji  徳島大学, 大学病院, 助教 (10403685)
MAEDA Kazuhisa  独立行政法人国立病院機構四国こどもとおとなの医療センター(臨床研究部(成育)、臨, 総合周産期母子医療センター, 部長 (30294680)
SUGANO Mikio  徳島大学, 大学病院, 医員 (70563807)
NAKAYAMA Taisuke  徳島大学, ヘルスバイオサイエンス研究部, 特別研究員 (80582791)
KUROBE Hirotsugu  徳島大学, ヘルスバイオサイエンス研究部, 助教 (30380083)
KINOSHITA Hajime  徳島大学, 大学病院, 特任助教 (80611227)
Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥18,330,000 (Direct Cost: ¥14,100,000、Indirect Cost: ¥4,230,000)
Fiscal Year 2014: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2012: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
Keywords臍帯血 / 細胞移植 / 未熟肺血管床
Outline of Final Research Achievements

Objectives: To investigate the effects of human umbilical cord blood-derived mononuclear cell (hUCB-MNC) transplantation on pulmonary hypertension (PH) induced by monocrotaline (MCT) in immunodeficient mice and their distribution. Methods: Fresh hUCB-MNCs harvested from a human donor after her delivery were injected intravenously into those PH mice (BALB/c Slc-nu/nu) 4 weeks after MCT induction. To reveal the incorporation into the lung, CMTMR-labeled hUCB-MNCs were observed in the lung. DiR-labeled hUCB-MNCs were detected in the lung and other organs by bioimaging. Results: PH was significantly improved 4 weeks after hUCB-MNC transplantation. CMTMR-positive hUCB-MNCs were observed in the lung 3 hours after transplantation. Bioluminescence signals were detected more strongly in the lung than in other organs for 24 hours after transplantation. Conclusions: The results indicate that hUCB-MNCs are incorporated into the lung early after hUCB-MNC transplantation and improve MCT-induced PH.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Annual Research Report
  • 2012 Annual Research Report
  • Research Products

    (39 results)

All 2014 2013 2012

All Journal Article (14 results) (of which Peer Reviewed: 14 results) Presentation (24 results) Book (1 results)

  • [Journal Article] Norwood手術におけるBlalock-Taussigシャント-実験から臨床への展開-2014

    • Author(s)
      北市 隆,菅野幹雄,木下 肇,中山泰介,黒部裕嗣,神原 保,藤本鋭貴,北川哲也
    • Journal Title

      胸部外科

      Volume: 67 Pages: 274-277

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 画像診断 Q&A2014

    • Author(s)
      北川哲也,曽我部仁史
    • Journal Title

      胸部外科

      Volume: 67 Pages: 477-482

    • NAID

      40020108856

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Role of macrophage-derived hypoxia-inducible factor (HIF)-1α as a mediator of vascular remodelling2013

    • Author(s)
      Nakayama T, Kurobe H, Sugasawa N, Kinoshita H, Higashida M, Matsuoka Y, Yoshida Y, Hirata Y, Sakata M, Maxfield M, Shimabukuro M, Takahama Y, Sata M, Tamaki T, Kitagawa T, Tomita S.
    • Journal Title

      Cardiovasc Res

      Volume: 99 Issue: 4 Pages: 705-715

    • DOI

      10.1093/cvr/cvt146

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] HMGB1 plays a critical role in vascular inflammation and lesion formation via toll-like receptor 92013

    • Author(s)
      Y. Hirata, H. Kurobe, M. Higashida, D. Fukuda, M. Shimabukuro, Y. Higashikuni, T. Kitagawa, M. Sata
    • Journal Title

      Atherosclerosis

      Volume: 231 Issue: 2 Pages: 227-233

    • DOI

      10.1016/j.atherosclerosis.2013.09.010

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Protective effects of selective mineralocorticoid receptor antagonist against aortic aneurysm progression in a novel murine model.2013

    • Author(s)
      Kurobe H, Hirata Y, Matsuoka Y, Sugasawa N, Higashida M, Nakayama T, Maxfield MW, Yoshida Y, Shimabukuro M, Kitagawa T, Sata M.
    • Journal Title

      J Surg Res

      Volume: 185 Issue: 1 Pages: 455-462

    • DOI

      10.1016/j.jss.2013.05.002

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Techniques and results in the management of multiple muscular trabecular ventricular septal defects.2013

    • Author(s)
      Kitagawa T, Kitaichi T, Sugano M, Kurobe H.
    • Journal Title

      Gen Thorac Cardiovasc Surg

      Volume: 61 Issue: 7 Pages: 367-375

    • DOI

      10.1007/s11748-013-0267-8

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Beneficial effect of a synthetic prostacyclin agonist, ONO-1301, in rat autoimmune myocarditis model2013

    • Author(s)
      Y. Hirata, H. Kurobe, E. Uematsu, S. Yagi, T. Soeki, H. Yamada, D. Fukuda, M. Shimabukuro, M. Nakayama, K. Matsumoto, Y. Sakai, T. Kitagawa, M. Sata
    • Journal Title

      Eur. J. Pharmacol.

      Volume: 699 Issue: 1-3 Pages: 81-87

    • DOI

      10.1016/j.ejphar.2012.11.045

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Complete but not partial thymectomy in early infancy reduces T-cell-mediated immune response: three-year tracing study after pediatric cardiac surgery2013

    • Author(s)
      Kurobe H, Tominaga T, Sugano M, Hayabuchi Y, Egawa Y, Takahama Y, Kitagawa T
    • Journal Title

      J Thorac Cardiovasc Surg

      Volume: 143 Issue: 3 Pages: 656-662

    • DOI

      10.1016/j.jtcvs.2012.12.015

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Azelnidipine suppresses the progression of aortic aneurysm in wild mice model through anti-inflammatory effects.2013

    • Author(s)
      Hirotsugu Kurobe
    • Journal Title

      The Journal of Thoracic and Cardiovascular Surgery

      Volume: in press Issue: 6 Pages: 271-277

    • DOI

      10.1016/j.jtcvs.2013.02.073

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Delayed-onset severe heparin-induced thrombocytopenia after total arch replacement under cardiopulmonary bypass2013

    • Author(s)
      Taisuke NAKAYAMA
    • Journal Title

      J Med Invest.

      Volume: 60 Pages: 154-158

    • NAID

      120005397333

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Epicardial adipose tissue volume and adipocytokine imbalance are strongly linked to human coronary atherosclerosis2013

    • Author(s)
      M. Shimabukuro, Y. Hirata, M. Tabata, M. Dagvasumberel, H. Sato, H. Kurobe, D. Fukuda, T. Soeki, T. Kitagawa, S. Takanashi, M. Sata
    • Journal Title

      Arterioscler. Thromb. Vasc. Biol.

      Volume: 33 Issue: 5 Pages: 1077-1084

    • DOI

      10.1161/atvbaha.112.300829

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Gastrointestinal hemorrhage caused by aorto-enteric fistula due to thoracoabdominal aortic aneurysm2013

    • Author(s)
      Taisuke Nakayama
    • Journal Title

      Asian Cardiovascular and Thoracic Annals

      Volume: (In press) )

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Gender disparities in the association between epicardial adipose tissue volume and coronary atherosclerosis: A 3-dimensional cardiac computed tomography imaging study in Japanese subjects2012

    • Author(s)
      Dagvasumberel M, Shimabukuro M, Nishiuchi T, Ueno J, Takao S, Fukuda D, Hirata Y, Kurobe H, Soeki T, Iwase T, Kusunose K, Niki T, Yamaguchi K, Taketani Y, Yagi S, Tomita N, Yamada H, Wakatsuki T, Harada M, Kitagawa T, Sata M
    • Journal Title

      Cardiovasc Diabetol

      Volume: 11 Issue: 1 Pages: 106-106

    • DOI

      10.1186/1475-2840-11-106

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Exendin-4, a glucagon-like peptide-1 receptor agonist, attenuates neointimal hyperplasia after vascular injury2012

    • Author(s)
      Hirata Y
    • Journal Title

      Eur J Pharmacol.

      Volume: 699 Pages: 106-111

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Presentation] 小児心臓手術時における経食道心エコーの合併症の検討2014

    • Author(s)
      北市 隆,菅野幹雄,木下 肇,中山泰介,黒部裕嗣,神原 保,藤本鋭貴,川人伸次,坂田美穗,早渕康信,北川哲也
    • Organizer
      第50回日本小児循環器学会総会・学術集会
    • Place of Presentation
      岡山コンベンションセンター(岡山県・岡山市)
    • Year and Date
      2014-07-03 – 2014-07-05
    • Related Report
      2014 Annual Research Report
  • [Presentation] 二尖性大動脈弁に対する大動脈弁置換術時の上行大動脈壁の問題点2014

    • Author(s)
      木下 肇,中山泰介,菅野幹雄,黒部裕嗣,神原 保,藤本鋭貴,北市 隆,北川哲也
    • Organizer
      第42回日本血管外科学会学術総会
    • Place of Presentation
      リンクステーションホール青森【青森市文化会館】・ホテル青森(青森県・青森市)
    • Year and Date
      2014-05-21 – 2014-05-23
    • Related Report
      2014 Annual Research Report
  • [Presentation] 傍腎動脈腹部大動脈瘤に対するEVARの検討2014

    • Author(s)
      藤本鋭貴,筑後文雄,中山泰介,木下 肇,菅野幹雄,黒部裕嗣,神原 保,割石精一郎,加納正志,北市 隆,北川哲也
    • Organizer
      第42回日本血管外科学会学術総会
    • Place of Presentation
      リンクステーションホール青森【青森市文化会館】・ホテル青森(青森県・青森市)
    • Year and Date
      2014-05-21 – 2014-05-23
    • Related Report
      2014 Annual Research Report
  • [Presentation] エゼチミブ投与が動脈リモデリング・機能へ与える影響の検討2014

    • Author(s)
      黒部裕嗣,菅澤典子,平田陽一郎,島袋充生,中山泰介,吉田恭史,松岡祐貴,木下 肇,佐田政隆,北川哲也
    • Organizer
      第42回日本血管外科学会学術総会
    • Place of Presentation
      リンクステーションホール青森【青森市文化会館】・ホテル青森(青森県・青森市)
    • Year and Date
      2014-05-21 – 2014-05-23
    • Related Report
      2014 Annual Research Report
  • [Presentation] 感染性腹部大動脈瘤に対して上腸間膜動脈にsnorkeling EVARを施行した1例2014

    • Author(s)
      木下 肇,中山泰介,菅野幹雄,黒部裕嗣,神原 保,藤本鋭貴,北市 隆,北川哲也
    • Organizer
      第42回日本血管外科学会学術総会
    • Place of Presentation
      リンクステーションホール青森【青森市文化会館】・ホテル青森(青森県・青森市)
    • Year and Date
      2014-05-21 – 2014-05-23
    • Related Report
      2014 Annual Research Report
  • [Presentation] Vascular remodeling effects by administrating Ezetimibe after arterial wire-injury in mice.2014

    • Author(s)
      Hirotsugu Kurobe, Noriko Sugasawa, Yoichiro Hirata, Mitsuo Shimabukuro, Taisuke Nakayama, Takeshi Yoshida, Mark W. Maxfield, Tetsuya Kitagawa
    • Organizer
      ASCVTS 2014
    • Place of Presentation
      ISTANBUL CONVENTION&EXHIBITION CENTER(ISTANBUL,TURKEY)
    • Year and Date
      2014-04-03 – 2014-04-06
    • Related Report
      2014 Annual Research Report
  • [Presentation] 臍帯血細胞移植は肺高血圧を改善させるか2014

    • Author(s)
      菅野幹雄、菅澤典子、黒部裕嗣、中山泰介、吉田 誉、北市 隆、木下 肇、神原 保、藤本鋭貴、苛原 稔 、北川哲也
    • Organizer
      第44回日本心臓血管外科学会学術総会
    • Place of Presentation
      ホテル日航熊本、熊本県民交流館パレア、鶴屋ホール(熊本県)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Macrophage-Specific Hypoxia-Inducible Factor (HIF)-1α-Deficient Mice Suppress the Vascular Remodeling and Regulate M2 Macrophage Polarization.2013

    • Author(s)
      Taisuke Nakayama, Hirotsugu Kurobe, Noriko Sugasawa, Hajime Kinoshita, Yasushi Yoshida, Yoichiro Hirata, Mie Sakata, Mark Webster Maxfield, Michio Shimabukuro, Yousuke Takahama, Masataka Sata, Toshiaki Tamaki, Tetsuya Kitagawa, Shuhei Tomita
    • Organizer
      American Heart Association AHA 2013
    • Place of Presentation
      Dallas Convention Center(アメリカ テキサス州ダラス市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Stanford B型大動脈解離に対するステントグラフト内挿術の手術時期の検討2012

    • Author(s)
      木下 肇
    • Organizer
      第65回日本胸部外科学会定期学術集会
    • Place of Presentation
      福岡国際会議場(福岡県)
    • Year and Date
      2012-10-17
    • Related Report
      2012 Annual Research Report
  • [Presentation] 小口径グラフト開存性改善を目的とする血管リモデリングにおけるマクロファージの基礎研究2012

    • Author(s)
      中山泰介
    • Organizer
      第65回日本胸部外科学会定期学術集会
    • Place of Presentation
      福岡国際会議場(福岡県)
    • Year and Date
      2012-10-17
    • Related Report
      2012 Annual Research Report
  • [Presentation] B型解離に対するステントグラフト治療の検討2012

    • Author(s)
      藤本鋭貴
    • Organizer
      第43回日本血管外科学会中国四国地方会
    • Place of Presentation
      ホテルセンチュリー21(広島県)
    • Year and Date
      2012-07-28
    • Related Report
      2012 Annual Research Report
  • [Presentation] 梗塞後心室中隔穿孔に対する手術時期と手術手技の工夫について2012

    • Author(s)
      木下 肇
    • Organizer
      第17回日本冠動脈外科学会
    • Place of Presentation
      ホテルグランドヒル市ヶ谷(東京都)
    • Year and Date
      2012-07-12
    • Related Report
      2012 Annual Research Report
  • [Presentation] 小児補助循環の問題点:中長期管理から離脱した2例から教訓2012

    • Author(s)
      中山泰介
    • Organizer
      第48回日本小児循環器学会総会
    • Place of Presentation
      国立京都国際会館(京都府)
    • Year and Date
      2012-07-05
    • Related Report
      2012 Annual Research Report
  • [Presentation] 総肺静脈還流異常症術後の左肺静脈狭窄に対するSnap back sutureless technique の適応と問題点2012

    • Author(s)
      北市 隆
    • Organizer
      第48回日本小児循環器学会総会
    • Place of Presentation
      国立京都国際会館(京都府)
    • Year and Date
      2012-07-05
    • Related Report
      2012 Annual Research Report
  • [Presentation] ファロー四微症における手術時期および術式について2012

    • Author(s)
      菅野幹雄
    • Organizer
      第48回日本小児循環器学会総会
    • Place of Presentation
      国立京都国際会館(京都府)
    • Year and Date
      2012-07-05
    • Related Report
      2012 Annual Research Report
  • [Presentation] LSVCを合併したDORVに対して自己心膜を用いた右房内re-routingを行い根治した1例2012

    • Author(s)
      木下 肇
    • Organizer
      第55回関西胸部外科学会学術集会
    • Place of Presentation
      大阪国際会議場(大阪府)
    • Year and Date
      2012-06-21
    • Related Report
      2012 Annual Research Report
  • [Presentation] Budd-Chiari症候群術後34年の下肢静脈瘤の1例2012

    • Author(s)
      木下 肇
    • Organizer
      第32回日本静脈学会総会
    • Place of Presentation
      大宮ソニックシティ(埼玉県)
    • Year and Date
      2012-06-06
    • Related Report
      2012 Annual Research Report
  • [Presentation] ステントグラフト治療後腎障害に対するスタチンの有用性2012

    • Author(s)
      中山泰介
    • Organizer
      第40回日本血管外科学会学術総会
    • Place of Presentation
      長野ビッグハット(長野県)
    • Year and Date
      2012-05-23
    • Related Report
      2012 Annual Research Report
  • [Presentation] 右室流出路再建後の肺動脈弁逆流に対する再手術例の検討2012

    • Author(s)
      北市 隆
    • Organizer
      第42回日本心臓血管外科学会学術総会
    • Place of Presentation
      秋田アトリオン・秋田キャッスルホテル(秋田県)
    • Year and Date
      2012-04-18
    • Related Report
      2012 Annual Research Report
  • [Presentation] 小口径グラフト開存性改善を目的とするマクロファージHIF制御に関しての基礎研究2012

    • Author(s)
      中山泰介
    • Organizer
      第42回日本心臓血管外科学会学術総会
    • Place of Presentation
      秋田アトリオン・秋田キャッスルホテル(秋田県)
    • Year and Date
      2012-04-18
    • Related Report
      2012 Annual Research Report
  • [Presentation] 肺循環系に問題のあるComplex症例(乳児期MAPCAsとabsent P valve)に対する治療戦略2012

    • Author(s)
      中山泰介
    • Organizer
      第42回日本心臓血管外科学会学術総会
    • Place of Presentation
      秋田アトリオン・秋田キャッスルホテル(秋田県)
    • Year and Date
      2012-04-18
    • Related Report
      2012 Annual Research Report
  • [Presentation] 活動期感染性心内膜炎に対する術前抗菌薬治療の検討2012

    • Author(s)
      木下 肇
    • Organizer
      第112回日本外科学会定期学術集会
    • Place of Presentation
      幕張メッセ(千葉県)
    • Year and Date
      2012-04-12
    • Related Report
      2012 Annual Research Report
  • [Presentation] ステントグラフト内挿術による術後遠隔期腎機能に対する影響の検討2012

    • Author(s)
      中山泰介
    • Organizer
      第112回日本外科学会定期学術集会
    • Place of Presentation
      幕張メッセ(千葉県)
    • Year and Date
      2012-04-12
    • Related Report
      2012 Annual Research Report
  • [Presentation] 弓部大動脈瘤に対するdebranching TEVAR の脳合併症について2012

    • Author(s)
      藤本鋭貴
    • Organizer
      第112回日本外科学会定期学術集会
    • Place of Presentation
      幕張メッセ(千葉県)
    • Year and Date
      2012-04-12
    • Related Report
      2012 Annual Research Report
  • [Book] 歯界展望 別冊『歯科医師のための医学ハンドブック』 Chapter1症候 チアノーゼ2014

    • Author(s)
      北市 隆,北川哲也
    • Total Pages
      224
    • Publisher
      医歯薬出版株式会社
    • Related Report
      2014 Annual Research Report

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Published: 2012-04-24   Modified: 2019-07-29  

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