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Analysis for circulating endothelial progenitor cells in moyamoya disease

Research Project

Project/Area Number 24390336
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Cerebral neurosurgery
Research InstitutionHokkaido University

Principal Investigator

HOUKIN Kiyohiro  北海道大学, 大学病院, 教授 (90229146)

Co-Investigator(Kenkyū-buntansha) KURODA Satoshi  富山大学, 大学院医学薬学研究部, 教授 (10301904)
SHICHINOHE Hideo  北海道大学, 大学病院, 助教 (80374479)
Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥18,070,000 (Direct Cost: ¥13,900,000、Indirect Cost: ¥4,170,000)
Fiscal Year 2014: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2013: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2012: ¥11,830,000 (Direct Cost: ¥9,100,000、Indirect Cost: ¥2,730,000)
Keywordsもやもや病 / 血管内皮前駆細胞 / iPS細胞 / サイトカイン
Outline of Final Research Achievements

1) Consumption of circulating EPC in MMD patients: Thirty-three patients with MMD and 14 healthy volunteers were registered to obtain mononuclear cells and plasma. In the patients, the circulating EPC was lower than control, and the circulating CD133+/CD34+ cells decreased after the operation, significantly. The level of bFGF was significantly lower in adult patients. The results suggested that EPCs would be consumed aggressively at the lesion. The pathological significance of bFGF in adult patients is still unclear.
2) Analysis of iPS cells from MMD patients: The iPS cell lines were established from PBMNCs of 3 MMD patients and 3 healthy persons. The endothelial differentiation was conducted on matrigel layer. The endothelial cells from MMD were impaired for the angiogenesis in vitro, significantly. In microarray analysis, it was found that KEGG pathway analysis of genes downregulated in MMD, that is, extracellular matrix receptor-related genes were significantly downregulated in MMD.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Annual Research Report
  • 2012 Annual Research Report
  • Research Products

    (23 results)

All 2015 2014 2013 2012 Other

All Journal Article (18 results) (of which Peer Reviewed: 18 results,  Open Access: 2 results,  Acknowledgement Compliant: 5 results) Presentation (3 results) Remarks (2 results)

  • [Journal Article] Therapeutic Effects of Human Multilineage-Differentiating Stress Enduring (MUSE) Cell Transplantation into Infarct Brain of Mice.2015

    • Author(s)
      Yamauchi T, Kuroda Y, Morita T, Shichinohe H, Houkin K, Dezawa M, Kuroda S.
    • Journal Title

      PLoS One

      Volume: 10(3) Issue: 3 Pages: e0116009-e0116009

    • DOI

      10.1371/journal.pone.0116009

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Neuroprotective effects of cilostazol are mediated by multiple mechanisms in a mouse model of permanent focal ischemia.2015

    • Author(s)
      Shichinohe H, Tan C, Abumiya T, Nakayama N, Kazumata K, Hokari M, Houkin K, Kuroda S.
    • Journal Title

      Brain Research

      Volume: 1602 Pages: 53-61

    • DOI

      10.1016/j.brainres.2015.01.022

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Chronic ischemia alters brain microstructural integrity and cognitive performance in adult moyamoya disease.2015

    • Author(s)
      Kazumata K, Tha KK, Narita H, Kusumi I, Shichinohe H, Ito M, Nakayama N, Houkin K.
    • Journal Title

      Stroke

      Volume: 46(2) Issue: 2 Pages: 354-360

    • DOI

      10.1161/strokeaha.114.007407

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Bone marrow stromal cells rescue ischemic brain by trophic effects and phenotypic change toward neural cells.2015

    • Author(s)
      Shichinohe H, Ishihara T, Takahashi K, Tanaka Y, Miyamoto M, Yamauchi T, Saito H, Takemoto H, Houkin K, Kuroda S.
    • Journal Title

      Neurorehabilitation and Neural Repair.

      Volume: 29(1) Issue: 1 Pages: 80-89

    • DOI

      10.1177/1545968314525856

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Short-, middle- and long-term safety of superparamagnetic iron oxide-labeled allogeneic bone marrow stromal cell transplantation in rat model of lacunar infarction.2014

    • Author(s)
      Tan C, Shichinohe H, Abumiya T, Nakayama N, Kazumata K, Hokari M, Hamauchi S, Houkin K.
    • Journal Title

      Neuropathology

      Volume: Epub ahead of print Issue: 3 Pages: 197-208

    • DOI

      10.1111/neup.12180

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Platelet lysate and granulocyte-colony stimulating factor serve safe and accelerated expansion of human bone marrow stromal cells for stroke therapy.2014

    • Author(s)
      Yamauchi T, Saito H, Ito M, Shichinohe H, Houkin K, Kuroda S.
    • Journal Title

      Translational Stroke Resarch

      Volume: 5(6) Issue: 6 Pages: 701-710

    • DOI

      10.1007/s12975-014-0360-z

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Application of cell sheet technology to bone marrow stromal cell transplantation for rat brain infarct.2014

    • Author(s)
      Ito M, Shichinohe H, Houkin K, Kuroda S.
    • Journal Title

      Journal of Tissue Engineering and Regenerative Medicine

      Volume: Epub ahead of print Issue: 2 Pages: 375-381

    • DOI

      10.1002/term.1920

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Spatial Relationship between Cerebral Microbleeds, Moyamoya Vessels, and Hematoma in Moyamoya Disease.2014

    • Author(s)
      Kazumata K, Shinbo D, Ito M, Shichinohe H, Kuroda S, Nakayama N, Houkin K.
    • Journal Title

      J Stroke Cerebrovasc Dis.

      Volume: Epub ahead of print Issue: 6 Pages: 1421-1428

    • DOI

      10.1016/j.jstrokecerebrovasdis.2013.12.007

    • Related Report
      2014 Annual Research Report 2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Bone Marrow Stromal Cells Rescue Ischemic Brain by Trophic Effects and Phenotypic Change Toward Neural Cells.2014

    • Author(s)
      Shichinohe H, Ishihara T, Takahashi K, Tanaka Y, Miyamoto M, Yamauchi T, Saito H, Takemoto H, Houkin K, Kuroda S.
    • Journal Title

      Neurorehabil Neural Repair.

      Volume: Epub ahead of print

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The frequency of postoperative stroke in moyamoya disease following combined revascularization: a single-university series and systematic review.2014

    • Author(s)
      Kazumata K, Ito M, Tokairin K, Ito Y, Houkin K, Nakayama N, Kuroda S, Ishikawa T, Kamiyama H.
    • Journal Title

      J Neurosurg

      Volume: Epub ahead of print

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Bone marrow stromal cell transplantation enhances recovery of motor function after lacunar stroke in rats.2013

    • Author(s)
      Shichinohe H, Yamauchi T, Saito H, Houkin K, Kuroda S.
    • Journal Title

      Acta Neurobiol Exp (Wars).

      Volume: 73(3) Pages: 354-363

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Ivy sign, misery perfusion, and asymptomatic moyamoya disease: FLAIR imaging and (15)O-gas positron emission tomography.2013

    • Author(s)
      Vuignier S, Ito M, Kurisu K, Kazumata K, Nakayama N, Shichinohe H, Shiga T, Kiss JZ, Tamaki N, Houkin K.
    • Journal Title

      Acta Neurochir (Wien).

      Volume: 155(11) Issue: 11 Pages: 2097-2104

    • DOI

      10.1007/s00701-013-1860-4

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 123I-iomazenil single photon emission computed tomography visualizes recovery of neuronal integrity by bone marrow stromal cell therapy in rat infarct brain.2013

    • Author(s)
      Saito H, Magota K, Zhao S, Kubo N, Kuge Y, Shichinohe H, Houkin K, Tamaki N, Kuroda S.
    • Journal Title

      Stroke

      Volume: 44(10) Issue: 10 Pages: 2869-2874

    • DOI

      10.1161/strokeaha.113.001612

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Incidence, locations, and longitudinal course of silent microbleeds in movamova disease : a Drospective T2*-weighted MRI study.2013

    • Author(s)
      Kuroda S, Kashiwazaki D, Ishikawa T, Nakavama N, Houkin K
    • Journal Title

      Stroke

      Volume: 44(2) Issue: 2 Pages: 516-518

    • DOI

      10.1161/strokeaha.112.678805

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effective Surgical Revascularization Improves Cerebral Hemodynamics and Resolves Headache in Pediatric Moyamoya Disease2012

    • Author(s)
      Kawabori M, Kuroda S, Nakayama N, Hirata K, Shiga T, Houkin K, Tamaki N.
    • Journal Title

      World Neurosurg.

      Volume: (e-pub) Issue: 5 Pages: 612-619

    • DOI

      10.1016/j.wneu.2012.08.005

    • NAID

      120006482586

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Predictors and Clinical Features of Postoperative Hyperperfusion after Surgical Revascularization for Moyamoya Disease : A Serial Single Photon Emission CT/Positron Emission Tomography Study.2012

    • Author(s)
      Uchino H, Kuroda S, Hirata K, Shiga T, Houkin K, Tamaki N.
    • Journal Title

      Stroke

      Volume: 43(10) Issue: 10 Pages: 2610-2616

    • DOI

      10.1161/strokeaha.112.654723

    • NAID

      10031131119

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Transient Crossed Cerebellar Diaschisis Due to Cerebral Hyperperfusion Following Surgical Revascularization for Moyamoya Disease2012

    • Author(s)
      Hokari M, Kuroda S, Simoda Y, Uchino H, Hirata K, Shiga T, Nakayama N, Houkin K, Tamaki N.
    • Journal Title

      Neurologia medico-chirurgica

      Volume: 52 Issue: 5 Pages: 350-353

    • DOI

      10.2176/nmc.52.350

    • NAID

      10030314688

    • ISSN
      0470-8105, 1349-8029
    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Bypass Surgery for Moyamoya Disease2012

    • Author(s)
      Kuroda S, Houkin K.
    • Journal Title

      Neurologia medico-chirurgica

      Volume: 52 Issue: 5 Pages: 287-294

    • DOI

      10.2176/nmc.52.287

    • NAID

      10030314424

    • ISSN
      0470-8105, 1349-8029
    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Presentation] Analysis for the role of angiogenic factors on endothelial cells derived from moyamoya disease-iPSCs2015

    • Author(s)
      Hamauchi S, Shichinohe H, Houkin K et al.
    • Organizer
      4th International Moyamoya Meeting
    • Place of Presentation
      Kaiserin-Friedrich-Haus(Berlin, Germany)
    • Year and Date
      2015-07-02 – 2015-07-04
    • Related Report
      2014 Annual Research Report
  • [Presentation] もやもや病iPS細胞由来血管内皮細胞におけるangiogenic factorへの反応性の解析2015

    • Author(s)
      浜内祝嗣、七戸秀夫、伊東雅基、長内俊也、穂刈正昭、中山若樹、数又研、江良択実、寳金清博
    • Organizer
      第14回日本再生医療学会総会
    • Place of Presentation
      パシフィコ横浜(横浜市)
    • Year and Date
      2015-03-19 – 2015-03-21
    • Related Report
      2014 Annual Research Report
  • [Presentation] 123I-iomazenil single photon emission computed tomography visualizes recovery of neuronal integrity by bone marrow stromal cell therapy in rat infarct brain.2013

    • Author(s)
      Saito H, Magota K, Zhao S, Kubo N, Kuge Y, Shichinohe H, Houkin K, Tamaki N, Kuroda S
    • Organizer
      WFNS XV World Congress of Neurosurgery
    • Place of Presentation
      COEX Convention Center (Seoul, South Korea)
    • Related Report
      2013 Annual Research Report
  • [Remarks] Hokkaido University Moyamoya Center

    • URL

      http://moyamoya.neurosurgery-hokudai.jp

    • Related Report
      2014 Annual Research Report
  • [Remarks] 北海道大学 大学院医学研究科・医学部 脳神経外科

    • URL

      http://www.neurosurgery-hokudai.jp

    • Related Report
      2014 Annual Research Report

URL: 

Published: 2012-04-24   Modified: 2019-07-29  

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