Analysis of regulatory mechanisms controlling proliferation and differentiation of chondrocytes
Project/Area Number |
24390354
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Partial Multi-year Fund |
Section | 一般 |
Research Field |
Orthopaedic surgery
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Research Institution | Kyoto University |
Principal Investigator |
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Project Period (FY) |
2012-04-01 – 2015-03-31
|
Project Status |
Completed (Fiscal Year 2015)
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Budget Amount *help |
¥16,380,000 (Direct Cost: ¥12,600,000、Indirect Cost: ¥3,780,000)
Fiscal Year 2014: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2013: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2012: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
|
Keywords | chondrocyte / Cyp26b1 / iPS cells / collagen / differentiation / 軟骨細胞 / 内軟骨性骨化 / iPS細胞 / ノックアウトマウス / SIK / regeneration / cartilage / dedifferentiation / knockout mice / Col11a2 / SIK3 |
Outline of Final Research Achievements |
We analyzed function of Cyp26b1 in chondrocytes by using conditional knockout mice which lack Cyp26b1 in chondrocyte-specific manner. The mice showed abnormal chondrocyte differentiation and cartilage formation, and short limbs. We also created iPS cell model from the disease in which chondrocyte differentiation is abnormal, and analyzed their pathology.
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Report
(4 results)
Research Products
(62 results)
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[Journal Article] Modeling type II collagenopathy skeletal dysplasia by directed conversion and induced pluripotent stem cells2015
Author(s)
Okada M, Ikegawa S, Morioka M, Yamashita A, Saito A, Sawai H,Murotsuki J, Ohashi H, Okamoto T, Nishimura G, Imaizumi K, Tsumaki N
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Journal Title
Hum Mol Genet
Volume: 24
Issue: 2
Pages: 299-313
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] Generation of Scaffoldless Hyaline Cartilaginous Tissue from Human iPSCs2015
Author(s)
Yamashita, A., Morioka, M., Yahara, Y., Okada, M., Kobayashi, T., Kuriyama, S., Matsuda, S., and Tsumaki, N
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Journal Title
Stem cell reports
Volume: 4
Issue: 3
Pages: 404-418
DOI
NAID
Related Report
Peer Reviewed / Open Access
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[Journal Article] Statin treatment rescues FGFR3 skeletal dysplasia phenotypes2014
Author(s)
Yamashita A, Morioka M, Kishi H, Kimura T, Yahara Y, Okada M, Fujita,K, Sawai H, Ikegawa S, Tsumaki N.
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Journal Title
Nature
Volume: 513(7519)
Issue: 7519
Pages: 507-11
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Cartilage intermediate layer protein promotes lumbar disc degeneration.2014
Author(s)
Seki S, Tsumaki N, Motomura H, Nogami M, Kawaguchi Y, Hori, T Suzuki K, Yahara Y, Higashimoto M, Oya T, Ikegawa S, Kimura T.
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Journal Title
Biochem Biophys Res Commun
Volume: 446
Issue: 4
Pages: 876-881
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Involvement of SIK3 in Glucose and Lipid Homeostasis in Mice2012
Author(s)
Uebi T, Itoh Y, Hatano O, Kumagai A, Sanosaka M, Sasaki T, Sasagawa S, Doi J, Tatsumi K, Mitamura K, Morii E, Aozasa K, Kawamura T, Okumura M, Nakae J, et al.
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Journal Title
PLoS One
Volume: 7
Issue: 5
Pages: e37803-e37803
DOI
Related Report
Peer Reviewed
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[Journal Article] An overgrowth disorder associated with excessive production of cGMP due to a gain-of-function mutation of the natriuretic peptide receptor 2 gene.2012
Author(s)
Miura K, Namba N, Fujiwara M, Ohata Y, Ishida H, Kitaoka T, Kubota T, Hirai H, Higuchi C, Tsumaki N, Yoshikawa H, Sakai N, Michigami T, Ozono K.
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Journal Title
PLoS One
Volume: 7
Issue: 8
Pages: e42180-e42180
DOI
Related Report
Peer Reviewed
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[Journal Article] Osterix regulates calcification and degradation of chondrogenic matrices through matrix metalloproteinase 13 (MMP13) expression in association with transcription factor Runx2 during endochondral ossification2012
Author(s)
Nishimura, R., Wakabayashi, M., Hata, K., Matsubara, T., Honma, S., Wakisaka, S. .Kiyonari, H., Shioi, G., Yamaguchi, A., Tsumaki, N., Akiyama, H. and Yoneda, T
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Journal Title
J. Biol. Chem
Volume: 287
Pages: 33179-33190
Related Report
Peer Reviewed
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