• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Investigation

Research Project

Project/Area Number 24390361
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Orthopaedic surgery
Research InstitutionMukogawa Women's University

Principal Investigator

WAKITANI Shigeyuki  武庫川女子大学, スポーツ健康科学部, 教授 (70243243)

Co-Investigator(Kenkyū-buntansha) TAKAGI Mutsumi  北海道大学, 工学研究科, 教授 (20263212)
HASHIMOTO Yusuke  大阪市立大学, 医学研究科, 講師 (10382178)
Co-Investigator(Renkei-kenkyūsha) TAMAMURA Sadahiro  武庫川女子大学, 健康スポーツ科学部, 博士研究員 (70431963)
Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥18,070,000 (Direct Cost: ¥13,900,000、Indirect Cost: ¥4,170,000)
Fiscal Year 2014: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
Fiscal Year 2013: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2012: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Keywords骨・軟骨代謝学 / 関節軟骨再生 / 骨髄間葉系幹細胞 / 軟骨細胞シート / 骨軟骨欠損 / 間葉系幹細胞 / 軟骨分化 / シート培養 / 浸透圧 / 軟骨修復 / 体性幹細胞 / 軟骨シート / 関節鏡視下手術 / 骨髄
Outline of Final Research Achievements

We previously reported some success repairing osteochondral defects with BMSC s, but did not regenerate new hyaline cartilage with the properties of native articular cartilage. To find better methods for regenerating articular cartilage defects using BMSCs, we hypothesized that the induction of chondrogenic differentiation and the seeding of many cells into the tissue matrix within the defect are important. We determined culture conditions that created stable scaffold-free cartilage-like cell-sheets from human BMSCs and assessed their effects after transplantation into osteochondral defects in nude rats. The results indicate that the FGF-2 enhances the proliferative and chondrogenic capacity of hBMSCs during expansion. Culture with FGF-2 with FBS may help to quickly generate sufficient numbers of cells to create stable and reliable scaffold-free cartilage-like cell-sheets, which contribute to the regeneration of osteochondral defects.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Annual Research Report
  • 2012 Annual Research Report
  • Research Products

    (17 results)

All 2015 2014 2013 2012

All Journal Article (4 results) (of which Peer Reviewed: 2 results) Presentation (13 results) (of which Invited: 2 results)

  • [Journal Article] 骨髄間葉系幹細胞からの軟骨様細胞シートの作製及び移植(前臨床試験)2015

    • Author(s)
      糸数万紀、目良恒、玉村禎宏、高木睦、脇谷滋之
    • Journal Title

      関節外科

      Volume: 34

    • Related Report
      2014 Annual Research Report
  • [Journal Article] Cartilage repair with autologous bone marrow mesenchymal stem cell transplantation: review of pre-clinical and clinical studies.2014

    • Author(s)
      Yamasaki S, Mera H, Itokazu M, Hashimoto Y, Wakitani S.
    • Journal Title

      Cartilage e-pub on May 19, 2014

      Volume: e-pub on May 19 Issue: 1 Pages: 2014-2014

    • DOI

      10.1177/1947603514551553

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Xeno-free and shrinkage-free preparation of scaffold-free cartilage-like disc-shaped cell sheet using human bone marrow mesenchymal stem cells.2013

    • Author(s)
      Yasushi Sato, Sigeyuki Wakitani, Mutsumi Takagi
    • Journal Title

      J. Biosci. Bioeng.

      Volume: 116 Pages: 734-739

    • NAID

      110009687838

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 骨髄間葉系細胞移植による軟骨再生治療2013

    • Author(s)
      目良恒、脇谷滋之
    • Journal Title

      Pharma Medica

      Volume: 31 Pages: 21-24

    • Related Report
      2013 Annual Research Report
  • [Presentation] Increase of Type 2 collagen Accumulation in Shrinkage-free Scaffold-free Cartilage-like MSCs Sheet.2014

    • Author(s)
      Sato Y, Wakitani S, Takagi M.
    • Organizer
      TERMIS-AM
    • Place of Presentation
      Washington DC(USA)
    • Year and Date
      2014-12-13 – 2014-12-16
    • Related Report
      2014 Annual Research Report
  • [Presentation] 骨髄間葉系幹細胞からの軟骨様シートの作成および移植2014

    • Author(s)
      糸数万紀、目良恒、中村博亮、高木睦、脇谷滋之
    • Organizer
      第29回日本整形外科学会基礎学術集会
    • Place of Presentation
      城山観光ホテル(鹿児島県鹿児島市)
    • Year and Date
      2014-10-09 – 2014-10-10
    • Related Report
      2014 Annual Research Report
  • [Presentation] ヒト骨髄細胞由来scafford-free再生軟骨様細胞シート作成の工夫2014

    • Author(s)
      糸数万紀、目良恒、佐藤康史、中村博亮、高木睦、脇谷滋之
    • Organizer
      第29回日本整形外科学会基礎学術集会
    • Place of Presentation
      城山観光ホテル(鹿児島県鹿児島市)
    • Year and Date
      2014-10-09 – 2014-10-10
    • Related Report
      2014 Annual Research Report
  • [Presentation] ヒト骨髄細胞(hBMSCs)由来scaffold-free再生軟骨細胞シート作成の工夫2014

    • Author(s)
      糸数万紀、目良恒、佐藤康史、中村博亮、高木睦、脇谷滋之
    • Organizer
      第6回日本関節鏡・膝・スポーツ整形外科学会
    • Place of Presentation
      広島国際会議場(広島県広島市)
    • Year and Date
      2014-07-24 – 2014-07-26
    • Related Report
      2014 Annual Research Report
  • [Presentation] The enhancement of human bone marrow derived mesenchymal stem cells (hBMSCs) for scaffold-free cartilage-like cell-sheets2014

    • Author(s)
      Itokazu M, Mera H, Sato Y, Nakamura H, Takagi M, Wakitani S.
    • Organizer
      60TH ANNNUAL MEETING of Osteoarthritis Research Society International 2014 world congress,
    • Place of Presentation
      Paris(France)
    • Year and Date
      2014-04-23
    • Related Report
      2014 Annual Research Report
  • [Presentation] ヒト骨髄細胞由来scafford-free再生軟骨様細胞シート作成の工夫2014

    • Author(s)
      糸数万紀、目良恒、佐藤康史、中村博亮、高木睦、脇谷滋之
    • Organizer
      第27回日本軟骨代謝学会
    • Place of Presentation
      京都府医師会館
    • Related Report
      2013 Annual Research Report
  • [Presentation] 骨髄間葉系幹細胞を用いた軟骨様細胞シートのECM蓄積量増大法の検討2013

    • Author(s)
      佐藤康史、笠井信宏、高橋伊織、脇谷滋之、高木睦
    • Organizer
      日本生物工学会年会広島大会
    • Place of Presentation
      広島国際会議場
    • Related Report
      2013 Annual Research Report
  • [Presentation] Clinical experience of stem cell implantation in an OA joint2013

    • Author(s)
      Wakitani S
    • Organizer
      1st Summit meeting of International Cartilage Repair Society
    • Place of Presentation
      Tallin
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Cartilage repair with autologous bone marrow mesenchymal stem cells in OA joints2013

    • Author(s)
      Wakitani S
    • Organizer
      11th World Congress of International Cartilage Repair Society
    • Place of Presentation
      Izmir
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Xeno-free Preparation of Scaffold-free Cartilage-like Cell Sheet using Bone Marrow Mesenchymal Stem Cells2012

    • Author(s)
      Sato S, Wakitani S, Takagi M
    • Organizer
      日本動物細胞工学会国際大会
    • Place of Presentation
      名古屋
    • Year and Date
      2012-11-30
    • Related Report
      2012 Annual Research Report
  • [Presentation] 隔膜培養器と骨髄間葉系幹細胞を用いたスキャフォールドフリー軟骨様細胞シート作製2012

    • Author(s)
      佐藤康史、脇谷滋之、高木睦
    • Organizer
      日本生物工学会
    • Place of Presentation
      神戸
    • Year and Date
      2012-10-25
    • Related Report
      2012 Annual Research Report
  • [Presentation] 隔膜培養器と骨髄間葉系幹細胞を用いたスキャフォールドフリー軟骨様細胞シート作製2012

    • Author(s)
      佐藤康史、脇谷滋之、高木睦
    • Organizer
      日本再生医療学会
    • Place of Presentation
      横浜
    • Year and Date
      2012-06-13
    • Related Report
      2012 Annual Research Report
  • [Presentation] 骨髄間葉系細胞を用いた関節軟骨再生2012

    • Author(s)
      脇谷滋之
    • Organizer
      日本整形外科学会
    • Place of Presentation
      京都
    • Year and Date
      2012-05-18
    • Related Report
      2012 Annual Research Report

URL: 

Published: 2012-04-24   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi