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Investigation into mechanisms of cancer aggressiveness by epithelial cells undergoing EMT

Research Project

Project/Area Number 24390419
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Pathobiological dentistry/Dental radiology
Research InstitutionUniversity of Yamanashi

Principal Investigator

SAITOH Masao  山梨大学, 大学院総合研究部, 教授 (90345041)

Research Collaborator ENDO Kaori  
SAKAMOTO Kei  
ISHII Hiroki  
HOSHI Yuta  
FUKAGAWA Akihiko  
FURUYA Satoshi  
MIYAZAWA Keiji  
KAWASAKI Tomonori  
Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥18,330,000 (Direct Cost: ¥14,100,000、Indirect Cost: ¥4,230,000)
Fiscal Year 2014: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2012: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000)
KeywordsEMT / TGF-beta / がん細胞 / シグナル伝達 / 上皮間葉転換 / 悪性形質 / がん / 浸潤 / 分子マーカー / TGF-β
Outline of Final Research Achievements

I performed experiments based on "Investigation into mechanisms of cancer aggressiveness by epithelial cells undergoing EMT". Using DNA microarray analyses, I explored several molecular marker proteins which are specifically expressed in epithelial cells undergoing EMT. I found that the marker proteins, Ret and Pai2, are expressed in aggressive cancer cells, whereas the marker protein (ITGA3) is in the most aggressive cells. However, ITGA3 was also expressed in fibroblast or mesenchymal cells in cancer microenvironment, as determined by immunohistochemical analyses. These findings have been published in articles and the details,includind gene Z, will be investigated by me and my collaborators.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Annual Research Report
  • 2012 Annual Research Report
  • Research Products

    (18 results)

All 2015 2014 2013 2012 Other

All Journal Article (6 results) (of which Peer Reviewed: 6 results,  Open Access: 4 results,  Acknowledgement Compliant: 2 results) Presentation (11 results) (of which Invited: 2 results) Remarks (1 results)

  • [Journal Article] STAT3 integrates cooperative Ras and TGF-β signals that induce Snail expression2015

    • Author(s)
      Masao Saitoh, Kaori Endo, Satoshi Furuya, Munetaka Minami, Akira Fukasawa, Takeshi Imamura, Keiji Miyazawa
    • Journal Title

      Oncogene

      Volume: 印刷中

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] The epithelial-mesenchymal transition is regulated at the post-transcriptional levels by TGF-β signaling during tumor progression2015

    • Author(s)
      Masao Saitoh
    • Journal Title

      Cancer Science

      Volume: 印刷中 Issue: 5 Pages: 481-488

    • DOI

      10.1111/cas.12630

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Basolateral BMP signaling in polarized epithelial cells2013

    • Author(s)
      Saitoh M, Shirakihara T, Fukasawa A, Horiguchi K, Sakamoto K, Sugiya H, Beppu H, Fujita Y, Morita I, Miyazono K, Miyazawa K.
    • Journal Title

      PLoS One

      Volume: 13 Issue: 5 Pages: 1-10

    • DOI

      10.1371/journal.pone.0062659

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Oligodendrocyte transcription factor 1 (Olig1) is a Smad cofactor involved in cell motility induced by transforming growth factor-β2013

    • Author(s)
      Motizuki M, Isogaya K, Miyake K, Ikushima H, Kubota T, Miyazono K, Saitoh M, Miyazawa K.
    • Journal Title

      Journal of Biological Chemistry

      Volume: 288 Issue: 26 Pages: 18911-18922

    • DOI

      10.1074/jbc.m113.480996

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Identification of integrin α3 as a molecular marker of cells undergoing epithelial-mesenchymal transition and of cancer cells with aggressive phenotypes.2013

    • Author(s)
      Shirakihara T, Kawasaki T, Fukagawa A, Semba K, Sakai R, Miyazono K, Miyazawa K, Saitoh M.
    • Journal Title

      Cancer Science

      Volume: 104 Issue: 9 Pages: 1189-1197

    • DOI

      10.1111/cas.12220

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Basolateral BMP signaling in polarized epithelial cells2013

    • Author(s)
      M Saitoh, et al
    • Journal Title

      PLOSONE

      Volume: (印刷中)

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Presentation] Roles of STAT3 in Snail induction by the synergism between Ras and TGF-β2015

    • Author(s)
      Masao Saitoh, Satoshi Furuya, Keiji Miyazawa
    • Organizer
      TGF-beta meeting
    • Place of Presentation
      筑波大学(茨城県・つくば市)
    • Year and Date
      2015-01-11 – 2015-01-13
    • Related Report
      2014 Annual Research Report
  • [Presentation] δEF1associates with DNMT1 and maintains DNA methylation of the E-cadherin promoter in breast cancer cells2014

    • Author(s)
      Masao Saitoh, Akihiko Fukagawa, Hiroki Ishii, Keiji Miyazawa
    • Organizer
      第73回日本癌学会総会
    • Place of Presentation
      パシフィコ横浜(神奈川県・横浜市)
    • Year and Date
      2014-09-25 – 2014-09-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] FGF-2 accelerates TGF-beta-induced EMT2013

    • Author(s)
      Masao Saitoh
    • Organizer
      AACR
    • Place of Presentation
      Omni SanDiego (アメリカ)
    • Year and Date
      2013-01-21
    • Related Report
      2012 Annual Research Report
  • [Presentation] Identification of integrin α3 as a molecular marker of cells undergoing epithelial-mesenchymal transition and of cancer cells with aggressive phenotypes.2013

    • Author(s)
      齋藤正夫
    • Organizer
      第55回歯科基礎医学会総会
    • Place of Presentation
      岡山コンベンションセンター( 岡山県岡山市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Identification of integrin α3 as a molecular marker of cells undergoing epithelial-mesenchymal transition and of cancer cells with aggressive phenotypes.2013

    • Author(s)
      Masao Saitoh
    • Organizer
      6th EMT meeting
    • Place of Presentation
      Hotel Melia (Alicante, Spain)
    • Related Report
      2013 Annual Research Report
  • [Presentation] role of TGF-b superfamily signals in EMT and epithelial cell polarity2013

    • Author(s)
      Masao Saitoh
    • Organizer
      第86回生化学会総会
    • Place of Presentation
      横浜コンチネンタルホテル( 神奈川県横浜市)
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Identification of integrin α3 as a molecular marker of cells undergoing EMT and of cancer cells with aggressive phenotypes2013

    • Author(s)
      Masao Saitoh
    • Organizer
      FASEB meeting
    • Place of Presentation
      The Steamboat Grand Resort (Steamboat SpringsSteamboat, USA)
    • Related Report
      2013 Annual Research Report
  • [Presentation] TGF-b誘導性EMTと癌微小環境2013

    • Author(s)
      斉藤正夫
    • Organizer
      第45回結合組織学会 第60回マトリックス研究会大会 合同大会
    • Place of Presentation
      和歌山県立医科大学( 和歌山県和歌山市)
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Roles of TGF-b superfamily signals in EMT and cancer progression2012

    • Author(s)
      Masao Saitoh
    • Organizer
      日本癌学会総会
    • Place of Presentation
      札幌文化会館(北海道)(招待講演)
    • Year and Date
      2012-09-21
    • Related Report
      2012 Annual Research Report
  • [Presentation] TGF-b誘導性EMTの分子機構2012

    • Author(s)
      Masao Saitoh
    • Organizer
      歯科基礎医学会総会
    • Place of Presentation
      奥羽大学(福島県)
    • Year and Date
      2012-09-15
    • Related Report
      2012 Annual Research Report
  • [Presentation] Role of BMP signaling in TGF-b mediated EMT2012

    • Author(s)
      Masao Saitoh
    • Organizer
      TGF-beta meeting
    • Place of Presentation
      Leiden大学(オランダ)
    • Year and Date
      2012-08-30
    • Related Report
      2012 Annual Research Report
  • [Remarks]

    • URL

      http://sangaku.yamanashi.ac.jp/SearchResearsher/contenrs/EF8E5AD567D62505.html

    • Related Report
      2012 Annual Research Report

URL: 

Published: 2012-04-24   Modified: 2019-07-29  

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