| Budget Amount *help |
¥18,460,000 (Direct Cost: ¥14,200,000、Indirect Cost: ¥4,260,000)
Fiscal Year 2015: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2014: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2013: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2012: ¥9,750,000 (Direct Cost: ¥7,500,000、Indirect Cost: ¥2,250,000)
|
|---|
| Outline of Final Research Achievements |
Connexin 43 is one of the gap junction protein. To understand the role of connexin 43 (Cx43) in salivary gland development, first we examined the expression of Cx43 by immunostaining using their specific antibody. Cx43 expressed terminal bud epithelum of salivary gland, but not in mesenchymal tisuue. In Cx43 knockout mice, branching morphogenesis of submandibular gland was dramatically inhibited. In presence of inhibitory peptide for Cx43 function, branching morphogenesis of salivary gland organ culture was also inhibited as similar to Cx43 knockout mice. From our results, Cx43 is necessary for salivary gland development and terminal bud branching. Further, gap junctional communication is important for the determination of cell fate. These information is useful for understanding the molecular mechanism of salivary gland development and regeneration.
|
