Making a cultured periosteal sheet advanced function by the blend with the demarcation making periodontal ligament cell and spreading out to the new treatment
| Project/Area Number |
24390465
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Periodontal dentistry
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| Research Institution | Niigata University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
KAWASE Tomoyuki 新潟大学, 医歯学系, 准教授 (90191999)
NAGATA Masaki 新潟大学, 医歯学系, 准教授 (10242439)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥18,460,000 (Direct Cost: ¥14,200,000、Indirect Cost: ¥4,260,000)
Fiscal Year 2014: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2013: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥9,620,000 (Direct Cost: ¥7,400,000、Indirect Cost: ¥2,220,000)
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| Keywords | 骨膜細胞 / 歯根膜細胞 / 共培養 / 骨組織再生 / 血管新生 |
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| Outline of Final Research Achievements |
For the preparation of more potent periosteal sheets, we examined the applicability of stem-cell culture medium. Periosteal sheets expanded with stem-cell culture medium, and formed thicker multilayers of cells. During this process, the surface marker CD146 was substantially upregulated. A representative osteoblastic marker, alkaline phosphatase was not upregulated by osteogenic induction. However, these expanded periosteal sheets exhibited substantially stronger osteogenic differentiation when implanted in nude mice. With respect of the effect of these expanded periosteal sheets on angiogenesis, we examined by using the experimental design such as implantation to mice or chorioallantoic membrane model (CAM) assay. In the latter experiment, angiogenesis and neovascularization was significantly increased. Moreover, the complex of the expanded periosteal sheets with human umbilical vein endothelial cells (HUVECs) has not been revealed a cooperative response.
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Report
(4 results)
Research Products
(35 results)
