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Studies on geographical distribution and genomic diversity of a new pathogenic Entamoeba species in Asia

Research Project

Project/Area Number 24406013
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section海外学術
Research Field Parasitology (including Sanitary zoology)
Research InstitutionTokai University

Principal Investigator

TACHIBANA Hiroshi  東海大学, 医学部, 教授 (10147168)

Co-Investigator(Kenkyū-buntansha) KOBAYASHI Seiki  慶應義塾大学, 医学部, 講師 (70112688)
KOMIYAMA Tomoyoshi  東海大学, 医学部, 准教授 (60439685)
MAKIUCHI Takashi  東海大学, 医学部, 助教 (80587709)
Project Period (FY) 2012-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
Fiscal Year 2015: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2014: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2013: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2012: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Keywords原虫 / Entamoeba nuttalli / マカク / 疫学 / 遺伝子多様性 / ゲノム / 人獣共通感染症 / アジア / Entamoeba / 中国 / タイ / ミャンマー / ネパール
Outline of Final Research Achievements

Entamoeba nuttalli, a new pathogenic species, was detected in fecal samples from rhesus macaques in Nepal and Myanmar, long-tailed macaques in Thailand, toque macaques in Sri Lanka, and Tibetan macaques in China. On the other hand, E. histolytica was not found in these samples. More than 80 strains of E. nuttalli were cultured successfully. Diversity of serine-rich protein genes and tRNA-linked short tandem repeat sequences was correlated with geographical distribution of host macaques. Draft genome sequence of E. nuttalli P19-061405 strain was determined.

Report

(5 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Annual Research Report
  • 2013 Annual Research Report
  • 2012 Annual Research Report

Research Products

(41 results)

All 2016 2015 2014 2013 2012 Other

All Int'l Joint Research (5 results) Journal Article (13 results) (of which Int'l Joint Research: 4 results,  Peer Reviewed: 11 results,  Open Access: 3 results,  Acknowledgement Compliant: 4 results) Presentation (21 results) (of which Invited: 1 results) Book (2 results)

  • [Int'l Joint Research] チュラロンコン大学(タイ)

    • Related Report
      2015 Annual Research Report
  • [Int'l Joint Research] 復旦大学/桂林医科大学(中国)

    • Related Report
      2015 Annual Research Report
  • [Int'l Joint Research] トリブバン大学/エベレスト国際臨床研究センター(ネパール)

    • Related Report
      2015 Annual Research Report
  • [Int'l Joint Research] サムラトランギ大学(インドネシア)

    • Related Report
      2015 Annual Research Report
  • [Int'l Joint Research] ミャンマー国立医学研究所/ヤンゴン工科大学(ミャンマー)

    • Related Report
      2015 Annual Research Report
  • [Journal Article] Evaluation of the C-terminal fragment of Entamoeba histolytica Gal/GalNAc lectin intermediate subunit as a vaccine candidate against amebic liver abscess2016

    • Author(s)
      Min X, Feng M, Guan Y, Man S, Fu Y, Cheng X, Tachibana H.
    • Journal Title

      PLoS Neglected Tropical Diseases

      Volume: 10

    • DOI

      10.1371/journal.pntd.0004419

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Comparative analysis of genotypic diversity in Entamoeba nuttalli isolates from Tibetan macaques and rhesus macaques in China2016

    • Author(s)
      Guan Y, Feng M, Cai J, Min X, Zhou X, Xu Q, Tan N, Cheng X, Tachibana H.
    • Journal Title

      Infection, Genetics and Evolution

      Volume: 38 Pages: 126-131

    • DOI

      10.1016/j.meegid.2015.12.014

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Isolation and molecular characterization of Entamoeba nuttalli strains showing novel isoenzyme patterns from wild toque macaques in Sri Lanka2016

    • Author(s)
      Tachibana H, Yanagi T, Feng M, Bandara KB, Kobayashi S, Cheng X, Hirayama K, Rajapakse RP.
    • Journal Title

      J. Eukaryot. Microbiol.

      Volume: 63 Pages: 1710180-1710180

    • DOI

      10.1111/jeu.12265

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Novel hemagglutinating, hemolytic and cytotoxic activities of the intermediate subunit of Entamoeba histolytica lectin2015

    • Author(s)
      Kato K, Yahata K, Gopal Dhoubhadel B, Fujii Y, Tachibana H
    • Journal Title

      Sci. Rep.

      Volume: 5 Pages: 13901-13901

    • DOI

      10.1038/srep13901

    • NAID

      120006986919

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Correlation between genotypes of tRNA-linked short tandem repeats in Entamoeba nuttalli isolates and the geographical distribution of host rhesus macaques2014

    • Author(s)
      Meng Feng, Tomoyoshi Komiyama, Tetsuo Yanagi, Xunjia Cheng, Jeevan B. Sherchand and Hiroshi Tachibana
    • Journal Title

      Parasitol. Res.

      Volume: 113 Pages: 367-374

    • DOI

      10.1007/s00436-013-3664-0

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Prevalence of Entamoeba nuttalli infection in wild rhesus macaques in Nepal and characterization of the parasite isolates2013

    • Author(s)
      Hiroshi Tachibana, Tetsuo Yanagi, Chamala Lama, Kishor Pandey, Meng Feng, Seiki Kobayashi and Jeevan B. Sherchand
    • Journal Title

      Parasitol. Int.

      Volume: 62 Pages: 230-235

    • DOI

      10.1016/j.parint.2013.01.004

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Prevalence and genetic diversity of Entamoeba species infecting macaques in southwest China2013

    • Author(s)
      Meng Feng, Junlong Cai, Xiangyang Min, Yonggeng Fu, Qing Xu, Hiroshi Tachibana and Xunjia Cheng
    • Journal Title

      Parasitol. Res.

      Volume: 112 Pages: 1529-1536

    • DOI

      10.1007/s00436-013-3299-1

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Primary pathogenicity analysis of a Chinese <i>Entamoeba histolytica </i>isolate2013

    • Author(s)
      Muxia Luo, Meng Feng, Xiangyang Min, Xueping Li, Junlong Cai, Hiroshi Tachibana and Xunjia Cheng
    • Journal Title

      BST

      Volume: 7 Issue: 2 Pages: 77-81

    • DOI

      10.5582/bst.2013.v7.2.77

    • NAID

      130005054649

    • ISSN
      1881-7815, 1881-7823
    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] An integrated microfluidic device for rapid serodiagnosis of amebiasis2013

    • Author(s)
      Wang Zhao, Li Zhang, Wenwen Jing, Sixiu Liu, Hiroshi Tachibana, Xunjia Cheng, Guodong Sui
    • Journal Title

      Biomicrofluidics

      Volume: Vol.7 Pages: 011101-011101

    • DOI

      10.1063/1.4793222

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Unique short tandem repeat nucleotide sequences in Entamoeba histolytica isolates from China2012

    • Author(s)
      Meng Feng, Junlong Cai, Bin Yang, Yongfeng Fu, Xiangyang Min, Hiroshi Tachibana, Xunjia Cheng
    • Journal Title

      Parasitology Research

      Volume: Vol.111 Pages: 1137-1142

    • DOI

      10.1007/s00436-012-2945-3

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Seroprevalence of Entamoeba histolytica infection in China2012

    • Author(s)
      Bin Yang, Yingdan Chen, Liang Wu, Longqi Xu, Hiroshi Tachibana, Xunjia Cheng
    • Journal Title

      American Journal of Tropical Medicine and Hygiene

      Volume: Vo.87 Pages: 97-103

    • DOI

      10.4269/ajtmh.2012.11-0626

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 腸管寄生原虫の遺伝子検査法2012

    • Author(s)
      吉川尚男, 他
    • Journal Title

      検査と技術

      Volume: 40 Pages: 478-486

    • Related Report
      2012 Annual Research Report
  • [Journal Article] 赤痢アメーバ感染症の2例 : Entamoeba dispar感染にヒトブラストシスチスおよび小形アメーバが検出されたブラジル人症例と回盲部に潰瘍を認めた無症候性E. histolytica感染の日本人症例2012

    • Author(s)
      山田 稔, 他
    • Journal Title

      Clinical Parasitology

      Volume: 23 Pages: 82-85

    • Related Report
      2012 Annual Research Report
  • [Presentation] スリランカのトクザルから分離した腸管寄生アメーバEntamoeba nuttalliにおける遺伝子多型と病原性の解析2015

    • Author(s)
      橘 裕司、柳 哲雄、馮 萌、Bandara K.B.A.T.、小林正規、程 訓佳、平山謙二、Rajapakse R.P.V.J.
    • Organizer
      第31回日本霊長類学会大会
    • Place of Presentation
      京都大学 百周年時計台記念館(京都府京都市)
    • Year and Date
      2015-07-19
    • Related Report
      2015 Annual Research Report
  • [Presentation] ミャンマーに生息するアカゲザルからのEntamoeba nuttalliの分離とその遺伝子多型解析2015

    • Author(s)
      橘裕司 他
    • Organizer
      第84回日本寄生虫学会大会
    • Place of Presentation
      杏林大学三鷹キャンパス
    • Year and Date
      2015-03-21
    • Related Report
      2014 Annual Research Report
  • [Presentation] ネパールのアカゲザルから分離した腸管寄生アメーバEntamoeba nuttalliにおける遺伝子多型の解析2014

    • Author(s)
      橘裕司 他
    • Organizer
      第30回日本霊長類学会大会
    • Place of Presentation
      大阪科学技術センタービル
    • Year and Date
      2014-07-05
    • Related Report
      2014 Annual Research Report
  • [Presentation] タイ中央部における野生カニクイザルからのEntamoeba nuttalliの分離とその性状解析2013

    • Author(s)
      橘 裕司, 他
    • Organizer
      第82回日本寄生虫学会大会
    • Place of Presentation
      東京医科歯科大学湯島キャンパス(東京都)
    • Year and Date
      2013-03-30
    • Related Report
      2012 Annual Research Report
  • [Presentation] Prevalence and genetic diversity of Entamoeba species infecting macaques in Southwest China2013

    • Author(s)
      Meng Feng, et al.
    • Organizer
      第82回日本寄生虫学会大会
    • Place of Presentation
      東京医科歯科大学湯島キャンパス(東京都)
    • Year and Date
      2013-03-30
    • Related Report
      2012 Annual Research Report
  • [Presentation] PCR法で診断されたアメーバ性肝膿瘍の2症例2013

    • Author(s)
      山田 稔, 他
    • Organizer
      第82回日本寄生虫学会大会
    • Place of Presentation
      東京医科歯科大学湯島キャンパス(東京都)
    • Year and Date
      2013-03-30
    • Related Report
      2012 Annual Research Report
  • [Presentation] Polymorphism of Enamoeba species isolated from humans and macaques in China2013

    • Author(s)
      Meng Feng, et al.
    • Organizer
      International Workshop on Enteric Protozoan Infections
    • Place of Presentation
      国立感染症研究所(東京都)
    • Year and Date
      2013-02-22
    • Related Report
      2012 Annual Research Report
  • [Presentation] Isolation and characterization of Enamoeba nuttalli from wild macaques in Asia2013

    • Author(s)
      Hiroshi Tachibana
    • Organizer
      International Workshop on Enteric Protozoan Infections
    • Place of Presentation
      国立感染症研究所(東京都)
    • Year and Date
      2013-02-22
    • Related Report
      2012 Annual Research Report
  • [Presentation] Primary culture for isolation of wild Enamoeba species2013

    • Author(s)
      Seiki Kobayashi
    • Organizer
      International Workshop on Enteric Protozoan Infections
    • Place of Presentation
      国立感染症研究所(東京都)
    • Year and Date
      2013-02-22
    • Related Report
      2012 Annual Research Report
  • [Presentation] ネパールのカトマンズにおける腸管寄生アメーバ感染の疫学研究2012

    • Author(s)
      橘 裕司, 他
    • Organizer
      第53回日本熱帯医学会大会
    • Place of Presentation
      とかちプラザ(北海道)
    • Year and Date
      2012-09-06
    • Related Report
      2012 Annual Research Report
  • [Presentation] Analysis of genotype information in Enamoeba histolytica isolates from China2012

    • Author(s)
      Meng Feng, et al.
    • Organizer
      第53回日本熱帯医学会大会
    • Place of Presentation
      とかちプラザ(北海道)
    • Year and Date
      2012-09-06
    • Related Report
      2012 Annual Research Report
  • [Presentation] 腸管寄生アメーバにおける新たなマトリョーシカ関係の同定と構築2012

    • Author(s)
      橘 裕司
    • Organizer
      第1回マトリョーシカ型生物研究会
    • Place of Presentation
      国立感染症研究所(東京都)
    • Year and Date
      2012-07-20
    • Related Report
      2012 Annual Research Report
  • [Presentation] タイのヒトとマカクにおける腸管寄生アメーバの感染状況とEntamoeba nuttalli分離株の性状解析

    • Author(s)
      橘裕司 他
    • Organizer
      第54回日本熱帯医学会大会
    • Place of Presentation
      長崎ブリックホール
    • Related Report
      2013 Annual Research Report
  • [Presentation] 腸管寄生アメーバ類におけるマトリョーシカ関係の同定と構築

    • Author(s)
      橘裕司
    • Organizer
      第2回マトリョーシカ型生物学研究会
    • Place of Presentation
      ホテル京都ガーデンパレス
    • Related Report
      2013 Annual Research Report
  • [Presentation] 次世代ならびに次々世代シーケンサーを用いた病原アメーバEntamoeba nuttalliの全ゲノムシークエンシング解析

    • Author(s)
      橘裕司 他
    • Organizer
      第83回日本寄生虫学会大会
    • Place of Presentation
      愛媛大学城北キャンパス
    • Related Report
      2013 Annual Research Report
  • [Presentation] 赤痢アメーバにおけるPRELI/MSF1p'ファミリータンパク質の解析

    • Author(s)
      牧内貴志,橘裕司
    • Organizer
      第83回日本寄生虫学会大会
    • Place of Presentation
      愛媛大学城北キャンパス
    • Related Report
      2013 Annual Research Report
  • [Presentation] Correlation between tRNA-linked short tandem repeats genes in Entamoeba nuttalli isolates and the geographical distribution of host macaques

    • Author(s)
      馮萌 他
    • Organizer
      第83回日本寄生虫学会大会
    • Place of Presentation
      愛媛大学城北キャンパス
    • Related Report
      2013 Annual Research Report
  • [Presentation] Amoebicidal activity of artemether by inhibition of phosphoserine aminotransferase synthesis in Acanthamoeba castellanii

    • Author(s)
      程訓佳 他
    • Organizer
      第83回日本寄生虫学会大会
    • Place of Presentation
      愛媛大学城北キャンパス
    • Related Report
      2013 Annual Research Report
  • [Presentation] 嫌気性原虫におけるオルガネラ異種間移植のためのマイクロインジェクション法の検討

    • Author(s)
      吉田和弘 他
    • Organizer
      第83回日本寄生虫学会大会
    • Place of Presentation
      愛媛大学城北キャンパス
    • Related Report
      2013 Annual Research Report
  • [Presentation] Polymorphism in and antibody responses to thrombospondin-related adhesive protein of Plasmodium vivax (PvTRAP)

    • Author(s)
      Kosuwin Rattiporn 他
    • Organizer
      第83回日本寄生虫学会大会
    • Place of Presentation
      愛媛大学城北キャンパス
    • Related Report
      2013 Annual Research Report
  • [Presentation] 赤痢アメーバ症の診断 近縁種との鑑別と多型解析

    • Author(s)
      橘裕司
    • Organizer
      静岡県寄生虫症研究会第18回研究総会
    • Place of Presentation
      アクトシティ浜松研修交流センター
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Book] Entamoeba. ウィルス・細菌・真菌・寄生虫同定便覧2014

    • Author(s)
      橘裕司
    • Publisher
      技術情報協会
    • Related Report
      2013 Annual Research Report
  • [Book] 赤痢アメーバ原虫の形質転換株の樹立法.寄生虫学研究 材料と方法 2013年版2013

    • Author(s)
      牧内貴志,橘裕司,野崎智義
    • Publisher
      三 恵社
    • Related Report
      2013 Annual Research Report

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Published: 2012-04-23   Modified: 2019-07-29  

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