Microbial and epidemiological study of aggressive periodontal disease in Moroccan adolescence.

• Project/Area Number: 24406035
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 海外学術
• Research Field: Social dentistry
• Research Institution: Kyushu Dental College
• Principal Investigator: ANSAI TOSHIHIRO 九州歯科大学, 歯学部, 教授 (80244789)
• Co-Investigator(Kenkyū-buntansha): MIYAZAKI Hideo 新潟大学, 歯学部, 教授 (00157629) ; YOSHIDA Akihiro 松本歯科大学, 歯学部, 教授 (20364151) ; YAMASHITA Yoshihisa 九州大学, 歯学部, 教授 (20192403) ; SOH Inho 九州歯科大学, 歯学部, 講師 (10285463) ; AWANO Shuji 九州歯科大学, 歯学部, 教授 (20301442)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥13,260,000 (Direct Cost: ¥10,200,000、Indirect Cost: ¥3,060,000); Fiscal Year 2014: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2013: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2012: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
• Keywords: Aa菌 / 若年性歯周病 / 海外疫学調査 / 歯周病 / A.a菌 / 海外学術調査

Outline of Final Research Achievements

Aggressive periodontitis are specifically involved in adolescence. However, there are no comprehensive studies which clarified the environmental and genetic factors in regard to aggressive periodontitis. This epidemiological study analyzed oral status, and properties of the patients using by questionnaire (e.g. age, sex, socioeconomic status, nutrition, and so on…). In genetic studies, we analyzed the existence of A. actinomycetemcomitans JP2 in subgingival plaque and molecular anthropological studies using by mitochondrial DNA sequences from blood cells.

Research Products

• [Journal Article] Proteome analysis identifies the Dpr protein of Streptococcus mutans as an important factor in the presence of early Streptococcal colonizers of tooth surfaces. (2015)
  • Author(s): Yoshida, A et al.
  • Journal Title: PLOS ONE Volume: 10 Issue: 3 Pages: e0121176-e0121176
  • DOI: 10.1371/journal.pone.0121176
  • NAID: 120005845335
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Monitoring the prevalence of viable and dead cariogenic bacteria in oral specimens and in vitro biofilms by qPCR combined with propidium monoazide. (2013)
  • Author(s): Yasunaga, A., Yoshida, A., Morikawa, K., Maki, K., Nakamura, S., Soh, I., Awano, S. and Ansai, T.
  • Journal Title: BMC Microbiology Volume: 13 Pages: 157-157
  • Peer Reviewed
• [Journal Article] Quantitative discrimination of Aggregatibacter actinomycetemcomitans highly leukotoxic JP2 clone from non-JP2 clones in diagnosis of aggressive periodontitis. (2012)
  • Author(s): Yoshida A, Ennibi OK, Miyazaki H, Hoshino T, Hayashida H, Nishihara T, Awano S, Ansai T.
  • Journal Title: BMC Infectious diseases Volume: 12 Issue: 1 Pages: 253-253
  • DOI: 10.1186/1471-2334-12-253
  • Peer Reviewed
• [Presentation] カリエスフリー小児からの新規抗う蝕細菌の同定 (2014)
  • Author(s): 中村 卓、ほか
  • Organizer: 日本口腔衛生学会
  • Place of Presentation: 熊本市
  • Year and Date: 2014-05-29 – 2014-05-31
• [Presentation] 歯面初期定着菌と共培養時に発現するStreptococcus mutanタンパク質の解析 (2013)
  • Author(s): 吉田明弘、安永愛、安細敏弘
  • Organizer: 第62回日本口腔衛生学会
  • Place of Presentation: 松本
• [Presentation] Aggregatibacter actinomycetemcomitansロイコトキシン産生/非産生株の選択的定量検出法の開発 (2012)
  • Author(s): 吉田明弘、ほか
  • Organizer: 第61回日本口腔衛生学会総会
  • Place of Presentation: 神奈川歯科大学
• [Presentation] Streptococcus anginosus βC-S lyaseによる組織傷害性の解析
  • Author(s): 中村卓、吉田明弘、星野倫範、吉田康夫、藤原卓、安細敏弘
  • Organizer: 第66回日本細菌学会九州地方会総会
  • Place of Presentation: 長崎
• [Book] Oral disease and hydrogen sulfide production by oral bacteria. (2013)
  • Author(s): Yoshida, A., Niki, M., Ansai, T. and Nakayama, K.
  • Total Pages: 210
  • Publisher: Nova Science
• [Book] DHEA in Human Health and Aging (2012)
  • Author(s): Yoshida, A., et al.
  • Total Pages: 452
  • Publisher: CRC Press, Taylor & Francis Group LLC.
• [Book] Periodontal Diseases A Clinician's Guide (2012)
  • Author(s): Yoshida, A., et al.
  • Total Pages: 368
  • Publisher: InTech
• [Remarks] 九州歯科大学地域健康開発歯学分野
  • URL: http://www2.kyu-dent.ac.jp/dept/oral-health
• [Remarks]
  • URL: http://www2.kyu-dent.ac.jp/dept/oral-health