Characterization of newborn interneurons in the mouse olfactory bulb

• Project/Area Number: 24500385
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Neuroscience in general
• Research Institution: Nara Medical University
• Principal Investigator: TAKAHASHI Hiroo 奈良県立医科大学, 医学部, 助教 (20390685)
• Co-Investigator(Kenkyū-buntansha): YOSHIHARA Sei-ichi 奈良県立医科大学, 医学部, 講師 (90360669)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2012: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: 嗅球 / 新生ニューロン / 介在ニューロン

Outline of Final Research Achievements

Olfactory bulb (OB) interneurons, such as periglomerular cells and granule cells, are generated in the subventricular zone of lateral ventricle and integrated into the pre-existing neural circuit throughout the life. However, it remains unknown how the interneurons migrate and are integrated into the pre-existing neural circuit within the OB.
To study the migration process of interneurons in the OB, we performed the time-lapse imaging of slice cultures. Although it was previously reported that blood vessels act as a scaffold for the interneurons, we found that some interneurons moved along blood vessels, but others utilized another interneuron as a scaffold to migrate radially within the OB. These results suggest that cell-cell interactions between interneurons play an important role of the neuronal migration in the OB.

Research Products

• [Journal Article] Characterization of newborn interneurons in the mouse olfactory bulb using postnatal electroporation. (2015)
  • Author(s): Takahashi H, Yoshihara S, Asahina R, Tamada Y and Tsuboi A.
  • Journal Title: Electroporation Methods in Neuroscience Volume: 102 Pages: 93-103
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Npas4 regulates Mdm2 and thus Dcx in experience-dependent olfactory bulb interneuron dendritic spine development. (2014)
  • Author(s): Yoshihara S, Takahashi H, Nishimura N, Kinoshita M, Asahina R, Kitsuki M, Tatsumi K, Furukawa-Hibi Y, Hirai H, Nagai T, Yamada K and Tsuboi A.
  • Journal Title: Cell Reports Volume: 8 Pages: 843-857
  • DOI: 10.1016/j.celrep.2014.06.056
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] Transcription factor Npas4 regulates the sensory experience-dependent development of dendritic spines in newborn olfactory bulb interneurons. (2014)
  • Author(s): 高橋 弘雄 他
  • Organizer: The 12th International Symposium on Molecular and Neural Mechanisms of Taste and Olfactory Perception
  • Place of Presentation: 九州大学
  • Year and Date: 2014-11-02 – 2014-11-03
• [Presentation] Time-lapse imaging of neuronal migration in the mouse olfactory bulb. (2013)
  • Author(s): 高橋 弘雄 他
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸ポートアイランド
• [Presentation] Time-lapse imaging of neuronal migration in the mouse olfactory bulb
  • Author(s): 高橋 弘雄 他
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡国際会議場（福岡県）
• [Remarks] 奈良県立医科大学脳神経システム医科学分野ホームページ
  • URL: http://www.naramed-u.ac.jp/~amrc-lab1/tsuboi%20Lab3.html
• [Remarks] 奈良医大坪井研ホームページ
  • URL: http://www.naramed-u.ac.jp/̃amrc-lab1/
• [Remarks] 奈良医大坪井研ホームページ
  • URL: http://www.naramed-u.ac.jp/~amrc-lab1/