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Analysis for a neural differentiation regulatory factor, Musashi1

Research Project

Project/Area Number 24500447
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Neurochemistry/Neuropharmacology
Research InstitutionKyushu University (2013-2014)
Keio University (2012)

Principal Investigator

HORISAWA Kenichi  九州大学, 生体防御医学研究所, 助教 (70424207)

Co-Investigator(Kenkyū-buntansha) 今井 貴雄  慶應義塾大学, 医学部生理学教室, 助教 (10383712)
芝田 晋介  慶應義塾大学, 医学部生理学教室, 講師 (70407089)
Co-Investigator(Renkei-kenkyūsha) 岡野 栄之  慶應義塾大学, 医学部生理学教室, 教授 (60160694)
土居 信英  慶應義塾大学, 理工学部生命情報学科, 准教授 (50327673)
Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
KeywordsMusashi1 / RNA結合タンパク質 / 神経発生 / 神経回路 / 未分化維持 / 翻訳制御 / 転写後制御 / 神経分化 / Musashi / Dcc / Dcx / 分化 / 幹細胞 / 再生医学
Outline of Final Research Achievements

Musashi1 (Msi1) is a RNA-binding, which is highly expressed in neural stem/progenitor cells and is responsible for stem cell maintenance and differentiation. The main function of Msi1 is to regulate the expression of its target mRNAs through translational up/down-regulation.
In search for novel Msi1 targets, in vitro SELEX selection was performed from a mouse embryonic brain mRNA library, and a 3’ UTR fragment of dcc was obtained. Dcc is a transmembrane receptor expressed on the growth cones of neurons, and it guides axons and cells in response to Netrin1.
In this project, in vitro and in cultured cell experiments clearly identified that the dcc mRNA is a direct target of Msi1, and is suppressed their translation through a canonical molecular machinery. Now, we are analyzing the connection between the molecular mechanism and neural development by using Msi1-KO mice.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report

Research Products

(25 results)

All 2015 2014 2013 2012

All Journal Article Presentation Book

  • [Journal Article] Connectomics: Comprehensive approaches for whole brain mapping2015

    • Author(s)
      Shibata S, Komaki Y, Seki F, Inouye MO, Nagai T, Okano H.
    • Journal Title

      Microscopy

      Volume: 64 Pages: 57-67

    • DOI

      10.1093/jmicro/dfu103

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Specific and quantitative labeling of biomolecules using click chemistry.2014

    • Author(s)
      Horisawa K.
    • Journal Title

      Front Physiol.

      Volume: 5 Pages: 457-457

    • DOI

      10.3389/fphys.2014.00457

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Rfx Transcription Factors Control Musashi1 Transcription in Mouse Neural Stem/Progenitor Cells2014

    • Author(s)
      Kawase S, Kuwako K, Imai T, Renault-Mihara F, Yaguchi K, Itohara S, Okano H.
    • Journal Title

      Stem Cells and Development

      Volume: 23 Pages: 2250-2261

    • DOI

      10.1089/scd.2014.0219

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Migration and differentiation of transplanted enteric neural crest-derived cells in murine model of Hirschsprung’s disease.2014

    • Author(s)
      Nishikawa R, Hotta R, Shimojima N, Shibata S, Nagoshi N, Nakamura M, Matsuzaki Y, Okano HJ, Kuroda T, Okano H, Morikawa Y.
    • Journal Title

      Cytotechnology

      Volume: Sep 18

    • DOI

      10.1007/s10616-014-9754-8

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Yb integrates piRNA intermediates and processing factors into perinuclear bodies to enhance piRISC assembly2014

    • Author(s)
      Murota Y, Ishizu H, Nakagawa S, Iwasaki YW, Shibata S, Kamatani MK, Saito K, Okano H, Siomi H, Siomi MC.
    • Journal Title

      Cell Rep

      Volume: 8 Pages: 103-113

    • DOI

      10.1016/j.celrep.2014.05.043

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Involvement of ER stress in dysmyelination of Pelizaeus-Merzbacher disease with PLP1 missense mutations shown by iPSC-derived oligodendrocytes.2014

    • Author(s)
      Numasawa-Kuroiwa Y, Okada Y, Shibata S, Kishi N, Akamatsu W, Shoji M, Nakanishi A, Oyama M, Oasaka H, Inoue K, Takahashi K, Yamanaka S, Kosaki K, Takahashi T, Okano H
    • Journal Title

      StemCellReports

      Volume: 2 Pages: 1-14

    • DOI

      10.1016/j.stemcr.2014.03.007

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Global gene expression analysis following spinal cord injury in non-human primates.2014

    • Author(s)
      Nishimura S, Sasaki T, Shimizu A, Yoshida K, Iwai H, Koya I, Kobayashi Y, Iakura G, Shibata S, Ebise H, Horiuchi K, Kudoh J, Toyama Y, Anderson AJ, Okano H, Nakamura M.
    • Journal Title

      Exp Neurol.

      Volume: 261 Pages: 171-179

    • DOI

      10.1016/j.expneurol.2014.05.021

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Involvement of ER stress in dysmyelination of Pelizaeus-Merzbacher disease with PLP1 missense mutations shown by iPSC-derived oligodendrocytes2014

    • Author(s)
      Kuroiwa-Numasawa Y, Okada Y, Shibata S, Kishi N, Akamatsu W, Shoji M, Nakanishi A, Oyama M, Osaka H, Inoue K, Takahashi K, Yamanaka S, Kosaki K, Takahashi T, Okano H
    • Journal Title

      Stem Cell Reports

      Volume: 未定

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Rewiring of regenerated axons by combining treadmill training with semaphorin3A inhibition.2014

    • Author(s)
      Zhang L, Kaneko S, Kikuchi K, Sano A, Maeda M, Kishino A, Shibata S, Mukaino M, Toyama Y, Liu M, Kimura T, Okano H, Nakamura M
    • Journal Title

      Molecular Brain

      Volume: 7 Pages: 14-14

    • DOI

      10.1186/1756-6606-7-14

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Inflammatory cascades mediate synapse elimination in spinal cord compression.2014

    • Author(s)
      Takano M, Kawabata S, Komaki Y, Shibata S, Hikishima K, Toyama Y, Okano H, Nakamura M.
    • Journal Title

      Journal of Neuroinflammation

      Volume: 11 Pages: 40-40

    • DOI

      10.1186/1742-2094-11-40

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] MIP-2A is a novel target of an anilinoquinazoline derivative for inhibition of tumour cell proliferation.2013

    • Author(s)
      Tokunaga M, Shiheido H, Tabata N, Sakuma-Yonemura Y, Takashima H, Horisawa K, Doi N, Yanagawa H.
    • Journal Title

      PLoS One.

      Volume: 8

    • DOI

      10.1371/journal.pone.0076774

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Hereditary spastic paraplegia protein spartin is an FK506-binding protein identified by mRNA display.2013

    • Author(s)
      Tokunaga M, Shiheido H, Hayakawa I, Utsumi A, Takashima H, Doi N, Horisawa K, Sakuma-Yonemura Y, Tabata N, Yanagawa H.
    • Journal Title

      Chemistry & Biology

      Volume: 20 Pages: 935-942

    • DOI

      10.1016/j.chembiol.2013.05.011

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] The long non-coding RNA nuclear-enriched abundant transcript 1_2 induces paraspeckle formation in the motor neuron during the early phase of amyotrophic lateral sclerosis.2013

    • Author(s)
      Nishimoto, Y., Nakagawa, S., Hirose, T., Okano, H. J., Takao, M., Shibata, S., Suyama, S., Kuwako, K., Imai, T., Murayama, S., Suzuki, N., and Okano, H
    • Journal Title

      Molecular Brain

      Volume: 6 Pages: 31-31

    • DOI

      10.1186/1756-6606-6-31

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Differential cellular localization of antioxidant enzymes in the trigeminal ganglion.2013

    • Author(s)
      Sato H, Shibata M, Shimizu T, Shibata S, Haruki T, Ebine T, Kuroi T, Iwashita T, Funakubo M, Kayama Y, Akazawa C, Wajima K, Nakagawa T. Okano H. Suzuki N
    • Journal Title

      Neuroscience.

      Volume: 248 Pages: 345-348

    • DOI

      10.1016/j.neuroscience.2013.06.010

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Sema3A regulates bone-mass accrual through sensory innervations.2013

    • Author(s)
      Fukuda T, Takeda S, Xu R, Ochi H, Sunamura S, Sato T, Shibata S, Yoshida Y, Gu Z, Kimura A, Ma C, Xu C, Bando W, Fujita K, Shinomiya K, Hirai T, Asou Y, Enomoto M, Okano H, Okawa A, Itoh H
    • Journal Title

      Nature

      Volume: 497 Pages: 490-3

    • DOI

      10.1038/nature12115

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] SOX10 is a novelmarker of acinus and intercalated duct differentiation in salivary gland tumors: a clue to the histogenesis for tumor diagnosis.2013

    • Author(s)
      Ohtomo R, Mori T, Shibata S, Tsuta K, Maeshima M, Akazawa C, Honda K, Yamada T, Yoshimoto S, Asai M, Okano H, Kanai Y, Tsuda H
    • Journal Title

      Mod Pathol

      Volume: Aug;26(8): Pages: 1041-1050

    • DOI

      10.1038/modpathol.2013.54

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Musashi-1 Post-Transcriptionally Enhances Phosphotyrosine-Binding Domain-Containing m-Numb Protein Expression in Regenerating Gastric Mucosa.2013

    • Author(s)
      Tetsufumi Takahashi
    • Journal Title

      PLoS One

      Volume: 8 Pages: 1-13

    • DOI

      10.1371/journal.pone.0053540

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] RNA-Binding Protein Musashi1 Modulates Glioma Cell Growth through the Post-Transcriptional Regulation of Notch and PI(3) Kinase/Akt Signaling Pathway.2012

    • Author(s)
      Muto J, Imai T, Ogawa D, Nishimoto Y, Okada Y, Mabuchi Y, Kawase T, Iwanami A, Mischel PS, Saya H, Yoshida K, Matsuzaki Y, Okano H
    • Journal Title

      PLoS One.

      Volume: 7(3)

    • DOI

      10.1371/journal.pone.0033431

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] RNA結合タンパク質による遺伝子制御と神経発生・神経機能2012

    • Author(s)
      今井貴雄、岡野栄之
    • Journal Title

      細胞工学

      Volume: 31 Pages: 668-674

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Presentation] A novel chemical tool for proteomic analysis of translational control2013

    • Author(s)
      Horisawa K., Miyazawa T., Ohkawa K., Oka K. and Doi N.
    • Organizer
      The 12th Human Proteome Organisation World Congress
    • Place of Presentation
      パシフィコ横浜(横浜市)
    • Related Report
      2013 Research-status Report
  • [Presentation] 翻訳調節による遺伝子発現制御の研究と解析技術の開発2013

    • Author(s)
      堀澤健一
    • Organizer
      筑波大学大学院生命環境科学研究科セミナー 動物細胞バイオテクノロジー
    • Place of Presentation
      筑波大学(つくば市)
    • Related Report
      2013 Research-status Report
    • Invited
  • [Presentation] RNA-binding protein Msi1 regulates the expression of an axon guidance receptor through translational repression2012

    • Author(s)
      Hara H., Horisawa K., Yanagawa H., Doi N.
    • Organizer
      Translational Control
    • Place of Presentation
      NewYork
    • Related Report
      2012 Research-status Report
  • [Presentation] 神経発生におけるRNA結合タンパク質Msi1による翻訳制御2012

    • Author(s)
      原裕恵、堀澤健一、松尾薫、柳川弘志、土居信英
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      福岡
    • Related Report
      2012 Research-status Report
  • [Book] 細胞工学別冊 ダイレクトリプログラミング :目的細胞別 遺伝子導入・培養条件・機能解析リファレンス2015

    • Author(s)
      堀澤健一、鈴木淳史(鈴木淳史・監修)
    • Total Pages
      119
    • Publisher
      学研メディカル秀潤社
    • Related Report
      2014 Annual Research Report
  • [Book] Neural Stem Cells and Therapy2012

    • Author(s)
      Horisawa K. and Yanagawa H.
    • Total Pages
      17
    • Publisher
      InTech
    • Related Report
      2012 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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