Analysis for a neural differentiation regulatory factor, Musashi1
Project/Area Number |
24500447
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Neurochemistry/Neuropharmacology
|
Research Institution | Kyushu University (2013-2014) Keio University (2012) |
Principal Investigator |
|
Co-Investigator(Kenkyū-buntansha) |
今井 貴雄 慶應義塾大学, 医学部生理学教室, 助教 (10383712)
芝田 晋介 慶應義塾大学, 医学部生理学教室, 講師 (70407089)
|
Co-Investigator(Renkei-kenkyūsha) |
岡野 栄之 慶應義塾大学, 医学部生理学教室, 教授 (60160694)
土居 信英 慶應義塾大学, 理工学部生命情報学科, 准教授 (50327673)
|
Project Period (FY) |
2012-04-01 – 2015-03-31
|
Project Status |
Completed (Fiscal Year 2014)
|
Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
|
Keywords | Musashi1 / RNA結合タンパク質 / 神経発生 / 神経回路 / 未分化維持 / 翻訳制御 / 転写後制御 / 神経分化 / Musashi / Dcc / Dcx / 分化 / 幹細胞 / 再生医学 |
Outline of Final Research Achievements |
Musashi1 (Msi1) is a RNA-binding, which is highly expressed in neural stem/progenitor cells and is responsible for stem cell maintenance and differentiation. The main function of Msi1 is to regulate the expression of its target mRNAs through translational up/down-regulation. In search for novel Msi1 targets, in vitro SELEX selection was performed from a mouse embryonic brain mRNA library, and a 3’ UTR fragment of dcc was obtained. Dcc is a transmembrane receptor expressed on the growth cones of neurons, and it guides axons and cells in response to Netrin1. In this project, in vitro and in cultured cell experiments clearly identified that the dcc mRNA is a direct target of Msi1, and is suppressed their translation through a canonical molecular machinery. Now, we are analyzing the connection between the molecular mechanism and neural development by using Msi1-KO mice.
|
Report
(4 results)
Research Products
(25 results)
-
-
-
-
[Journal Article] Migration and differentiation of transplanted enteric neural crest-derived cells in murine model of Hirschsprung’s disease.2014
Author(s)
Nishikawa R, Hotta R, Shimojima N, Shibata S, Nagoshi N, Nakamura M, Matsuzaki Y, Okano HJ, Kuroda T, Okano H, Morikawa Y.
-
Journal Title
Cytotechnology
Volume: Sep 18
DOI
Related Report
Peer Reviewed
-
-
[Journal Article] Involvement of ER stress in dysmyelination of Pelizaeus-Merzbacher disease with PLP1 missense mutations shown by iPSC-derived oligodendrocytes.2014
Author(s)
Numasawa-Kuroiwa Y, Okada Y, Shibata S, Kishi N, Akamatsu W, Shoji M, Nakanishi A, Oyama M, Oasaka H, Inoue K, Takahashi K, Yamanaka S, Kosaki K, Takahashi T, Okano H
-
Journal Title
StemCellReports
Volume: 2
Pages: 1-14
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
-
[Journal Article] Global gene expression analysis following spinal cord injury in non-human primates.2014
Author(s)
Nishimura S, Sasaki T, Shimizu A, Yoshida K, Iwai H, Koya I, Kobayashi Y, Iakura G, Shibata S, Ebise H, Horiuchi K, Kudoh J, Toyama Y, Anderson AJ, Okano H, Nakamura M.
-
Journal Title
Exp Neurol.
Volume: 261
Pages: 171-179
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
-
[Journal Article] Involvement of ER stress in dysmyelination of Pelizaeus-Merzbacher disease with PLP1 missense mutations shown by iPSC-derived oligodendrocytes2014
Author(s)
Kuroiwa-Numasawa Y, Okada Y, Shibata S, Kishi N, Akamatsu W, Shoji M, Nakanishi A, Oyama M, Osaka H, Inoue K, Takahashi K, Yamanaka S, Kosaki K, Takahashi T, Okano H
-
Journal Title
Stem Cell Reports
Volume: 未定
Related Report
Peer Reviewed
-
[Journal Article] Rewiring of regenerated axons by combining treadmill training with semaphorin3A inhibition.2014
Author(s)
Zhang L, Kaneko S, Kikuchi K, Sano A, Maeda M, Kishino A, Shibata S, Mukaino M, Toyama Y, Liu M, Kimura T, Okano H, Nakamura M
-
Journal Title
Molecular Brain
Volume: 7
Pages: 14-14
DOI
Related Report
Peer Reviewed
-
-
-
-
[Journal Article] The long non-coding RNA nuclear-enriched abundant transcript 1_2 induces paraspeckle formation in the motor neuron during the early phase of amyotrophic lateral sclerosis.2013
Author(s)
Nishimoto, Y., Nakagawa, S., Hirose, T., Okano, H. J., Takao, M., Shibata, S., Suyama, S., Kuwako, K., Imai, T., Murayama, S., Suzuki, N., and Okano, H
-
Journal Title
Molecular Brain
Volume: 6
Pages: 31-31
DOI
Related Report
Peer Reviewed
-
[Journal Article] Differential cellular localization of antioxidant enzymes in the trigeminal ganglion.2013
Author(s)
Sato H, Shibata M, Shimizu T, Shibata S, Haruki T, Ebine T, Kuroi T, Iwashita T, Funakubo M, Kayama Y, Akazawa C, Wajima K, Nakagawa T. Okano H. Suzuki N
-
Journal Title
Neuroscience.
Volume: 248
Pages: 345-348
DOI
Related Report
Peer Reviewed
-
[Journal Article] Sema3A regulates bone-mass accrual through sensory innervations.2013
Author(s)
Fukuda T, Takeda S, Xu R, Ochi H, Sunamura S, Sato T, Shibata S, Yoshida Y, Gu Z, Kimura A, Ma C, Xu C, Bando W, Fujita K, Shinomiya K, Hirai T, Asou Y, Enomoto M, Okano H, Okawa A, Itoh H
-
Journal Title
Nature
Volume: 497
Pages: 490-3
DOI
Related Report
Peer Reviewed
-
[Journal Article] SOX10 is a novelmarker of acinus and intercalated duct differentiation in salivary gland tumors: a clue to the histogenesis for tumor diagnosis.2013
Author(s)
Ohtomo R, Mori T, Shibata S, Tsuta K, Maeshima M, Akazawa C, Honda K, Yamada T, Yoshimoto S, Asai M, Okano H, Kanai Y, Tsuda H
-
Journal Title
Mod Pathol
Volume: Aug;26(8):
Pages: 1041-1050
DOI
Related Report
Peer Reviewed
-
-
[Journal Article] RNA-Binding Protein Musashi1 Modulates Glioma Cell Growth through the Post-Transcriptional Regulation of Notch and PI(3) Kinase/Akt Signaling Pathway.2012
Author(s)
Muto J, Imai T, Ogawa D, Nishimoto Y, Okada Y, Mabuchi Y, Kawase T, Iwanami A, Mischel PS, Saya H, Yoshida K, Matsuzaki Y, Okano H
-
Journal Title
DOI
Related Report
Peer Reviewed
-
-
-
-
-
-
-