Biosynthetic pathway to neuromelanin and its aging process
Project/Area Number |
24500450
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Neurochemistry/Neuropharmacology
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Research Institution | Fujita Health University |
Principal Investigator |
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Co-Investigator(Kenkyū-buntansha) |
ITO Shosuke 藤田保健衛生大学, 名誉教授 (70121431)
|
Project Period (FY) |
2012-04-01 – 2015-03-31
|
Project Status |
Completed (Fiscal Year 2014)
|
Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
Keywords | ニューロメラニン / 黒質 / 青斑核 / 化学分析 / ノルエピネフリン / ドーパミン / パーキンソン病 / 国際情報交換 / イタリア / アメリカ / 化学分解反応 / ヨウ化水素還元 |
Outline of Final Research Achievements |
Neuromelanin (NM) occurs in dopaminergic neurons of the substantia nigra (SN) and in noradrenergic neurons of the locus coeruleus (LC). HPLC analysis after HI hydrolysis of natural NM detected AHPEA, AHPAA and AHEB, which confirmed that SN-NM and LC-NM contain melanin derived not only from DA and NE, but also from several other catecholic metabolites, such as DOPA, DOPAC, DOMA, DOPE and DOPEG, in addition to the corresponding Cys-derivatives in varying degrees. However, HI hydrolysis showed that LC-NM produced the same degradation products as were detected in SN-NM. Thus, we needed to develop a new chemical detection method to validate the existence of NE in LC-NM. HCl hydrolysis of LC-NM in the presence of thioglycolic acid yields new products arising from substitution of the hydroxyl group by thioglycolic acid at the benzyl position of NE and cysteinyl-NE. This is the first chemical evidence showing that NE and cysteinyl-NE are incorporated into LC-NM.
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Report
(4 results)
Research Products
(46 results)
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[Journal Article] Reduction of the nitro group to amine by hydroidoic acid to synthesize o-aminophenol derivatives as putative degradative markers of neuromelanin.2014
Author(s)
Wakamatsu, K., Tanaka, H., Tabuchi, K., Ojika, M., Zucca, F.A., Zecca, L., Ito, S.
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Journal Title
Molecules
Volume: 19
Issue: 6
Pages: 8039-8050
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] TLR4 and NLRP3 inflammasome activation in monocytes by N-propionyl cysteaminylphenol-maleimide-dextran (NPCMD).2014
Author(s)
Mizote, Y., Wakamatsu, K., Ito, S., Uenaka, A., Ohue, Y., Kurose, K., Isobe, M., Ito, A., Tamura, Y., Honda, H., Yamashita, T., Nohara, S., Oka, M., Jimbow, K., Nakayama, E.
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Journal Title
J. Dermatol. Sci.
Volume: 73
Issue: 3
Pages: 76-87
DOI
Related Report
Peer Reviewed
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[Journal Article] Sex-specific phenotype integration: endocrine profiles, coloration and behavior in fledgling boobies.2014
Author(s)
Fargallo, J.A., Velando, A., L., Lopez-Rull, I., Ganan, N., Lifshiz, N., Wakamatsu, K., Torres, R.
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Journal Title
Behavioral Ecology
Volume: 25
Issue: 1
Pages: 76-87
DOI
Related Report
Peer Reviewed
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[Journal Article] Heterospecific female mimicry in ficedula flycatchers.2014
Author(s)
Calhim, S., Adamik, P., Jarvisto, P, P., Leskinen, P., Torok, J., Wakamatsu, K., Laaksonen, T.
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Journal Title
J. Evol. Biol.
Volume: 27
Issue: 3
Pages: 660-666
DOI
Related Report
Peer Reviewed
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[Journal Article] Variants in melanogenesis-related genes associate with skin cancer risk among Japanese populations.2014
Author(s)
Yoshizawa J, Abe Y, Oiso N, Fukai K, Hozumi Y, Nakamura T, Narita T, Motokawa T, Wakamatsu K, Ito S, Kawada A, Tamiya G, Suzuki T
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Journal Title
J Dermatol
Volume: 41
Issue: 4
Pages: 296-302
DOI
Related Report
Peer Reviewed
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[Journal Article] Photoaging of human retinal pigment epithelium is accompanied by oxidative modifications of its eumelanin2013
Author(s)
S Ito, A Pilat, W Gerwat, CMB Skumatz, M Ito, A Kiyono, A Zadlo, Y Nakanishi, L Kolbe, JM Burke, T Sarna, K Wakamatsu
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Journal Title
Pigment Cell Melanoma Res
Volume: 26
Issue: 3
Pages: 357-366
DOI
Related Report
Peer Reviewed
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[Journal Article] Sexual dimorphism in melanin pigmentation, feather coloration and its heritability in the barn swallow (Hirundo rustica).2013
Author(s)
Saino, N., Romano, M., Rubolini, D., Teplitsky, C., Ambrosini, R., Caprioli, M., Canova, L., Wakamatsu, K.
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Journal Title
Plos One
Volume: 8
Issue: 2
Pages: e58024-e58024
DOI
Related Report
Peer Reviewed
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[Journal Article] Viability is associated with melanin-based colouration in the barn swallow (Hirundo rustica).2013
Author(s)
Saino, N., Romano, M., Rubolini, D., Ambrosini, R., Caprioli, M., Milzani, A., Costanzo, A., Colombo, G., Canova, L., Wakamatsu, K.
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Journal Title
Plos One
Volume: 8
Issue: 4
Pages: e60426-e60426
DOI
Related Report
Peer Reviewed
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[Journal Article] Melanins and melanogenesis: methods, standards, protocols2013
Author(s)
d’Ischia, M., Wakamatsu, K., Napolitano, A., Briganti, S., Garcia-Borron, J-C., Kovacs, D., Meredith, P., Pezzella, A., Picardo, M., Sarna, T., Simon, J.D., Ito, S.
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Journal Title
Pigment Cell Melanoma Res.
Volume: 26
Issue: 5
Pages: 616-633
DOI
Related Report
Peer Reviewed
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[Journal Article] Adaptation of pelage color and pigment variations in Israel subterranean blind mole rats, Splax ehrenbergi.2013
Author(s)
Singaravelan, N., Ratz, S., Tzur, S., Belifante, S., Pavlicek, T., Beiles, A., Ito, S., Wakamatsu, K., Nevo, E.
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Journal Title
Plos One
Volume: 8
Issue: 7
Pages: e69346-e69346
DOI
Related Report
Peer Reviewed
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[Journal Article] Raman Spectroscopy as a non-invasive technique for the quantification of melanins in feathers and hairs.2013
Author(s)
Galvan, I., Jorge, A., Ito, K., Tabuchi, K., Solano, F., Wakamatsu, K.
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Journal Title
Pigment Cell melanoma Res.
Volume: 26
Pages: 917-923
Related Report
Peer Reviewed
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[Journal Article] Impact of diagenesis and maturation on the survival of eumelanin in the fossil record2013
Author(s)
K Glass, S Ito, PR Wilby, T Sota, A Nakamura, CR Bowers, KE Miller, S Dutta, RE Summons, DEG Briggs, K Wakamatsu, JD Simon
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Journal Title
Org Geochem
Volume: 64
Pages: 29-37
DOI
Related Report
Peer Reviewed
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[Journal Article] Photoaging of human retinal pigment epithelium is accompanied by oxidative modification of its eumelanin2013
Author(s)
Ito, S., Pilat, A., Gerwat W., Skumatz, C.M.B., Ito, M., Kiyono, A., Zadlo, A., Nakanishi, Y., Kolbe, L., Burke, J.M., Sarna, T., Wakamatsu, K.
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Journal Title
Pigment Cell Melanoma Res
Volume: 26
Pages: 357-366
Related Report
Peer Reviewed
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[Journal Article] Neuromelanins of human brain have soluble and insoluble components with dolichols attached to the melanic structure.2012
Author(s)
Engelen, M., Vanna, R., Bellei, C., Zucca, F. A., Wakamatsu, K., Monzani, E., Ito. S., Casella, L., Zecca, L.
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Journal Title
PLoS One
Volume: 7
Issue: 11
Pages: e48490-e48490
DOI
Related Report
Peer Reviewed
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[Journal Article] Direct chemical evidence for eumelanin pigment from the Jurassic period.2012
Author(s)
Glass, K., Ito, S., Wilby, P.R., Sota, T., Nakamura, A., Bowers, C.R., Vinther, J., Dutta, S., Summons, R., Briggs, D.E., Wakamatsu, K., Simon, J.D.
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Journal Title
Proc. Natl. Acad. Sci. USA
Volume: 109
Pages: 10218-10223
Related Report
Peer Reviewed
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