Optical control of mouse olfactory sensory neurons
Project/Area Number |
24500456
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Neurophysiology and muscle physiology
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Research Institution | Tokyo Medical and Dental University |
Principal Investigator |
ISHII TOMOHIRO 東京医科歯科大学, 医歯(薬)学総合研究科, 助教 (60549947)
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Project Period (FY) |
2012-04-01 – 2015-03-31
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Project Status |
Completed (Fiscal Year 2014)
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Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
|
Keywords | 光遺伝学 / カルシウムシグナル / オプトジェネティクス / カルシウムチャネル |
Outline of Final Research Achievements |
Ca2+ signals are highly regulated in a spatiotemporal manner in numerous cellular physiological events. Here, I engineered blue light-activated Ca2+ channel switch (BACCS), as an optogenetic tool for manipulating Ca2+ signals. Ca2+ influx was induced in various cell types expressing BACCS. Local Ca2+ signals were generated in neurites of mouse hippocampal neurons by local exposure to light. In the mouse olfactory system, neurons expressing BACCS showed electrophysiological responses in response to light, suggesting that it is applicable to in vivo studies. Thus, BACCSs will be a useful optogenetic tool for controlling intracellular Ca2+ both in vitro and in vivo.
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Report
(4 results)
Research Products
(7 results)
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[Journal Article] A family of nonclassical class I MHC genes contributes to ultrasensitive chemodetection by mouse vomeronasal sensory neurons.2014
Author(s)
Leinders-Zufall T*, Ishii T*, Chamero P, Hendrix P, Oboti L, Schmid A, Kircher S, Pyrski M, Akiyoshi S, Khan M, Vaes E, Zufall F, Mombaerts P.
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Journal Title
Journal of Neuroscience
Volume: 34
Issue: 15
Pages: 5121-5133
DOI
Related Report
Peer Reviewed
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