Analysis of MG56: sarcoplasmic reticulum-resident protein

• Project/Area Number: 24500470
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Neurophysiology and muscle physiology
• Research Institution: Kyoto University
• Principal Investigator: NISHI Miyuki 京都大学, 健康長寿社会の総合医療開発ユニット, 准教授 (60183894)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 骨格筋 / カルシウム / 小胞体

Outline of Final Research Achievements

Mitsugumin 56 (MG56), also known as the membrane-bound O-acyl-transferase family member hedgehog acyltransferase-like, was identified as a new sarcoplasmic reticulum component in striated muscle. Mg56-knockout mice grew normally for a week after birth, but shortly thereafter exhibited a suckling defect and died under starvation conditions. In the knockout skeletal muscle, regular contractile features were largely preserved, but sarcoplasmic reticulum elements swelled and further developed enormous vacuoles. In parallel, the unfolded protein response was severely activated in the knockout muscle, and presumably disrupted muscle development leading to the suckling failure. Therefore, MG56 seems essential for postnatal skeletal muscle maturation.

Research Products

• [Journal Article] Mitsugumin 56 (hedgehog acyltransferase-like) is a sarcoplasmic reticulum-resident protein essential for postnatal muscle maturation. (2015)
  • Author(s): Van B, Nishi M, Komazaki S, Ichimura A, Kakizawa S, Nakanaga K, Aoki J, Park KH, Ma J, Ueyama T, Ogata T, Maruyama N, Takeshima H.
  • Journal Title: FEBS Lett. Volume: in press Issue: 10 Pages: 1095-1104
  • DOI: 10.1016/j.febslet.2015.03.028
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] TRIM72 is required for effective repair of alveolar epithelial cell wounding. (2014)
  • Author(s): Kim SC, Kellett T, Wang S, Nishi M, Nagre N, Zhou B, Flodby P, Shilo K, Ghadiali SN, Takeshima H, Hubmayr RD, Zhao X.
  • Journal Title: Am J Physiol Lung Cell Mol Physiol. Volume: 307 Issue: 6 Pages: L449-L459
  • DOI: 10.1152/ajplung.00172.2014
  • Peer Reviewed / Open Access
• [Journal Article] Treatment of acute lung injury by targeting MG53-mediated cell membrane repair. (2014)
  • Author(s): Jia Y, Chen K, Lin P, Lieber G, Nishi M, Yan R, Wang Z, Yao Y, Li Y, Whitson BA, Duann P, Li H, Zhou X, Zhu H, Takeshima H, Hunter JC, McLeod RL, Weisleder N, Zeng C, Ma J.
  • Journal Title: Nat Commun. Volume: 5 Issue: 1 Pages: 1-12
  • DOI: 10.1038/ncomms5387
  • Peer Reviewed / Open Access
• [Journal Article] TRIC-B channels display labile gating: evidence from the TRIC-A knockout mouse model. (2013)
  • Author(s): Venturi, E., Matyjaszkiewicz, A., Pitt, S. J., Tsaneva-Atanasova, K., Nishi, M., Yamazaki, D., Takeshima, H. & Sitsapesan, R.
  • Journal Title: Pflugers Arch. Volume: 465 Issue: 8 Pages: 1135-1148
  • DOI: 10.1007/s00424-013-1251-y
  • Peer Reviewed
• [Journal Article] Enhanced dihydropyridine receptor calcium channel activity restores muscle strength in JP45/CASQ1 double knockout mice. (2013)
  • Author(s): Mosca B, Delbono O, Laura Messi M, Bergamelli L, Wang ZM, Vukcevic M, Lopez R, Treves S, Nishi M, Takeshima H, Paolini C, Martini M, Rispoli G, Protasi F, Zorzato F.
  • Journal Title: Nat. Commun. Volume: 4 Pages: 1541-1549
  • Peer Reviewed
• [Journal Article] Endogenously determined restriction of food intake overcomes excitation-contraction uncoupling in JP45KO mice with aging (2012)
  • Author(s): Delbono, O., Messi M. L., Wang, Z., Treves, S., Mosca, B., Bergamelli, L., Nishi, M., Takeshima, H., Shi H., Xue B. & Zorzato, F
  • Journal Title: Exp. Gerontol Volume: 4 Issue: 4 Pages: 304-316
  • DOI: 10.1016/j.exger.2012.01.004
  • Peer Reviewed
• [Journal Article] Recombinant human MG53 protein modulates therapeutic cell membrane repair. (2012)
  • Author(s): Weisleder N, Takizawa N, Lin P, Wang X, Cao C, Zhang Y, Tan T, Ferrante C, Zhu H, Chen P-J, Yan R, Sterling M, Zhao X, Hwang M, Cai C, Cheng H, Takeshima H, Xiao R. & Ma J.
  • Journal Title: Sci. Transl. Med. Volume: 4 Issue: 139 Pages: 1-10
  • DOI: 10.1126/scitranslmed.3003921
  • Peer Reviewed
• [Journal Article] TRIM50 regulates vesicular trafficking for acid secretion in gastric parietal cells. (2012)
  • Author(s): Nishi, M., Aoyama, F., Kisa, F., Zhu, H., Sun, M., Lin, P., Ohta, H., Yamamoto, S., Kakizawa, S., Sakai, H., Ma, J., Sawaguchi, A. & Takeshima, H.
  • Journal Title: J. Biol. Chem. Volume: 287 Issue: 40 Pages: 33523-33532
  • DOI: 10.1074/jbc.m112.370551
  • Peer Reviewed
• [Presentation] Mitsugumin 56 is an MBOAT family member and contributes to postnatal muscle maturation (2015)
  • Author(s): Nishi M, Van B, Komazaki S, Yamazaki D, Park KH, Ma J, Takeshima H
  • Organizer: biophysical society 59th annual meeting
  • Place of Presentation: Baltimore, USA
  • Year and Date: 2015-02-07 – 2015-02-11
• [Presentation] The MBOAT family protein Mitsugumin 56 contributes to postnatal maturation in the muscle sarcoplasmic reticulum. (2014)
  • Author(s): Van, B., Nishi, M., Komazaki, S., Park, K. H., Yamazaki, D., Ma, J. & Takeshima, H.
  • Organizer: Biophysical Society 58th annual meeting
  • Place of Presentation: Sanfrancisco, CA, USA
• [Presentation] TRIM50 regulates ventricular trafficking for acid secretion in gastric parietal cells. (2013)
  • Author(s): Nishi, M., Aoyama, F., Kisa, F., Zhu, H., Sun, M., Lin, P., Van, Bo., Ma, J., Sawaguchi, A., & Takeshima, H.
  • Organizer: Biophysical Society 57th annual Meeting
  • Place of Presentation: Philadelphia, USA
• [Remarks] 京都大学大学院薬学研究科生体分子認識学分野
  • URL: http://www.pharm.kyoto-u.ac.jp//biochem/