| Project/Area Number |
24500604
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Rehabilitation science/Welfare engineering
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| Research Institution | Wakayama Medical University |
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Principal Investigator |
KAMI Katsuya 和歌山県立医科大学, 医学部, 博士研究員 (20204612)
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| Co-Investigator(Kenkyū-buntansha) |
SENBA Emiko 和歌山県立医科大学, 医学部, 博士研究員 (00135691)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 神経障害性疼痛 / トレッドミル走 / ヒラメ筋 / 筋線維横断面積 / HDAC1 / アセチル化ヒストンH3K9 / ミクログリア / エピジェネティクス / 疼痛の緩和 / 自発運動 / GABA / GAD65/67 / ヒストン脱アセチル化酵素 / ヒストンアセチル化酵素 / 大腰筋 / マクロファージサブタイプ |
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| Outline of Final Research Achievements |
The present study investigated the influence of neuropathic pain (NPP) on the lower extremity skeletal muscle and treadmill running on NPP. Mice that were subjected to partial sciatic nerve ligation (PSL) showed neuropathic pain behavior and significant enlargement and reduction of myofiber cross sectional area (CSA) in the contralateral and ipsilateral soleus muscles, respectively. Treadmill running for 5 days after the PSL significantly improved pain behaviors of NPP model mice, and also increased the number of microglia with nuclear expression of acetylated histone H3K9 in the ipsilateral superficial dorsal horn. In this way, although NPP produces laterality in the soleus muscle, treadmill running may be effective in correcting the unbalanced soleus muscle. In addition, the present study suggested that epigenetic modification such as hyperacetylation of histone H3K9 in microglia in the ipsilateral dorsal horn may play a role in producing exercise-induced hypoalgesia.
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