Exploration of food functionality of Dioscorea japonica targeting lipid mediator
Project/Area Number |
24500948
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Eating habits, studies on eating habits
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Research Institution | Okayama Prefectural University |
Principal Investigator |
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Co-Investigator(Renkei-kenkyūsha) |
INOUE Hiroyasu 奈良女子大学, 生活環境学部, 教授 (40183743)
YAMAMOTO Kei 財団法人東京都医学総合研究所, 主席研究員 (30304504)
KAWAKAMI Yuki 岡山県立大学, 保健福祉学部, 助教 (30453202)
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Project Period (FY) |
2012-04-01 – 2015-03-31
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Project Status |
Completed (Fiscal Year 2014)
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Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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Keywords | 脂質メディエーター / PGE2 / COX-2 / mPGES-1 / 炎症 / 癌 / 自然薯 / 食品機能性 / 抗炎症 / 抗腫瘍 |
Outline of Final Research Achievements |
In this study, we investigated novel functions of Dioscorea japonica on the related enzymes synthesizing prostaglandin (PG) E2. PGE2 is one of the lipid mediators, and is involved in many pathophysiological conditions such as inflammation and tumorigenesis. Under the conditions, PGE2 is synthesized from arachidonic acid by cyclooxygenase (COX)-2 and microsomal PGE synthase (mPGES)-1. Dioscorea japonica extract (DJE) suppressed COX-2 and mPGES-1 in carcinoma A549 and Caco-2 cells, and inflammatory RAW264 cells. DJE induced cancer cells to apoptosis, and suppressed inflammatory cytokines. In mouse model of squamous cell carcinoma of the skin, Dioscorea japonica inhibited tumor formation, suppressed COX-2 and mPGES-1, and decreased subepidermal eosinophils and neutrophils. These results indicate that Dioscorea japonica may have preventive effects against inflammation and tumorigenesis via suppression of PGE2 synthesis pathway.
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Report
(4 results)
Research Products
(45 results)
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[Journal Article] Neutralization of leukotriene C4 and D4 activity by monoclonal and single-chain antibodies.2014
Author(s)
Kawakami Y, Hirano S, Kinoshita M, Otsuki A, Suzuki-Yamamoto T; Suzuki M, Kimoto M, Sasabe S, Fukushima M, Kishimoto K, Izumi T, Oga T, Narumiya S, Sugahara M, Miyano M, Yamamoto S, Takahashi Y.
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Journal Title
Biochim Biophys Acta.
Volume: 1840
Issue: 6
Pages: 1625-1633
DOI
Related Report
Peer Reviewed
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[Presentation] Dioscorea japonica extract suppresses cyclooxygenase-2 and microsomal prostaglandin E synthase-1 in lung carcinoma A549 cells.2015
Author(s)
Keisuke Toda, Izumi Tsukayama, Takuto Mega, Yuka Konoike, Toshiya Arakawa, Yuki Kawakami, Masumi Kimoto, Yoshitaka Takahashi, Toshiko Suzuki-Yamamoto.
Organizer
14th International Conference of Bioactive Lipids in Cancer, Inflammation and Related Diseases.
Place of Presentation
Budapest, Hungary.
Year and Date
2015-07-12 – 2015-07-15
Related Report
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[Presentation] Preventive effect of Dioscorea japonica on squamous cell carcinoma of the mouse skin involving down-regulation of prostaglandin E2 synthetic pathway.2015
Author(s)
Izumi Tsukayama, Yasunori Takeda , Takuto Mega, Keisuke Toda, Yuki Kawakami, Yoshitaka Takahashi, Masumi Kimoto, Kei Yamamoto, Makoto Murakami, Toshiko Suzuki-Yamamoto.
Organizer
14th International Conference of Bioactive Lipids in Cancer, Inflammation and Related Diseases.
Place of Presentation
Budapest, Hungary.
Year and Date
2015-07-12 – 2015-07-15
Related Report
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[Presentation] Purification and characterization of bovine lipocalin-type prostaglandin D synthase from cow’s milk.2015
Author(s)
Toshiko Suzuki-Yamamoto, Mitsuki Tanaka, Erika Kawai, Keisuke Toda, Yui Serai, Takuto Mega, Yuki Kawakami, Yoshitaka Takahashi, Kei Yamamoto, Makoto Murakami, Masumi Kimoto.
Organizer
14th International Conference of Bioactive Lipids in Cancer, Inflammation and Related Diseases.
Place of Presentation
Budapest, Hungary.
Year and Date
2015-07-12 – 2015-07-15
Related Report
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[Presentation] Preventive effect of Dioscorea japonica on squamous cell carcinoma of mouse skin.2015
Author(s)
Yasunori Takeda, Izumi Tsukayama, Takuto Mega, Keisuke Toda, Mitsuki Tanaka, Yuki Kawakami, Yoshitaka Takahashi, Kei Yamamoto, Makoto Murakami, Toshiko Suzuki-Yamamoto.
Organizer
12th Asian Congress of Nutrition
Place of Presentation
Yokohama, Japan.
Year and Date
2015-05-14 – 2015-05-18
Related Report
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[Presentation] Dioscorea japonica extract suppresses cyclooxygenase-2 and microsomal prostaglandin E synthase-1 in lung carcinoma A549 cells and macrophage-like RAW264 cells.2015
Author(s)
Keisuke Toda, Izumi Tsukayama, Takuto Mega, Mitsuki Tanaka, Yasunori Takeda, Toshiya Arakawa, Yuki Kawakami, Masumi Kimoto, Yoshitaka Takahashi, Toshiko Suzuki-Yamamoto.
Organizer
12th Asian Congress of Nutrition
Place of Presentation
Yokohama, Japan.
Year and Date
2015-05-14 – 2015-05-18
Related Report
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[Presentation] Dioscorea japonica suppresses COX-2 and mPGES-1 expression and has preventive effects on squamous cell carcinoma.2015
Author(s)
Izumi Tsukayama, Yasunori Takeda, Kanako Nakatani, Takuto Mega, Keisuke Toda, Yuki Kawakami, Yoshitaka Takahashi, Toshiya Arakawa, Kei Yamamoto, Makoto Murakami, Toshiko Suzuki-Yamamoto.
Organizer
6th International Conference on Phosphplipaze A2 and Lipid Medoators (PLM2015)
Place of Presentation
Tokyo, Japan.
Year and Date
2015-02-10 – 2015-02-12
Related Report
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[Presentation] Inhibition of leukocyte-type 12-lipoxygenase activity by Chinese tea.2014
Author(s)
Yoshiko Mori, Akemi Otsuki, Shuji Okochi, Kensuke Okamoto, Yuki Kawakami, Toshiko Suzuki-Yamamoto, Hideaki Tsuji, Hideyuki Ito, Ding Zhi Fang, Yoshitaka Takahashi.
Organizer
The Conference on Food and Nutrition Science among Sichuan University, Nanchang University, Woosong University, and Okayama Prefectural University
Place of Presentation
Chengdu, China.
Year and Date
2014-09-12
Related Report
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[Presentation] Monoclonal and single-chain antibodies neutralize biological activities of leukotriene C4 and D4.2014
Author(s)
Yuki Kawakami, Mai Kinoshita, Yoshiko Mori, Shuji Okochi, Kensuke Okamoto, Sae Sasabe, Toshiko Suzuki-Yamamoto, Hiromi Yamashita, Masumi Kimoto, Mitsuo Fukushima, Hideaki Tsuji, Yoshitaka Takahashi.
Organizer
The Conference on Food and Nutrition Science among Sichuan University, Nanchang University, Woosong University, and Okayama Prefectural University
Place of Presentation
Chengdu, China.
Year and Date
2014-09-12
Related Report
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[Presentation] 40S ribosomal protein subunit 2 is a major arginine methylated protein in rat tissues.2014
Author(s)
Ryosuke Morimoto, Toshiko Suzuki-Yamamoto, Sayaka Yamamoto, Nao Nomura, Yoshitaka Takahashi, Hiromi Yamashita, Masumi Kimoto.
Organizer
The Conference on Food and Nutrition Science among Sichuan University, Nanchang University, Woosong University, and Okayama Prefectural University
Place of Presentation
Chengdu, China.
Year and Date
2014-09-12
Related Report
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[Presentation] Dioscorea japonica extract suppressed the expression of cyclooxygenase-2 and microsomal prostaglandin E synthase-1 in lung carcinoma A549 cells and macrophage-like RAW 264 cells.2014
Author(s)
Izumi Tsukayama, Takuto Mega, Keisuke Toda, Yasunori Takeda, Yuki Kawakami, Masumi Kimoto, Yoshitaka Takahashi, Toshiko Suzuki-Yamamoto.
Organizer
The Conference on Food and Nutrition Science among Sichuan University, Nanchang University, Woosong University, and Okayama Prefectural University
Place of Presentation
Chengdu, China.
Year and Date
2014-09-12
Related Report
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[Presentation] Preventive effect of Dioscorea japonica on squamous cell carcinoma of the skin.2014
Author(s)
Toshiko Suzuki-Yamamoto, Izumi Tsukayama, Miki Takafuji, Takuto Mega, Kanako Nakatani, Yuri Wada, Sayaka Hamasaki, Makiko Suzuki, Yuki Kawakami, Yoshitaka Takahashi.
Organizer
Okayama Prefectural University-Nanchang University Workshop for Food and Nutritional Sciences,
Place of Presentation
Okayama, Japan
Related Report
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[Presentation] Dioscorea japonica extract suppresses the expression of cyclooxygenase-2 and microsomal prostaglandin E synthase-1 in lung carcinoma A549 cells and macrophage-like RAW264 cells.2014
Author(s)
Izumi Tsukayama, Sayuri Tanaka, Risako Takiuchi, Miki Takafuji, Keisuke Toda, Mitsuki Tanaka, Toshiya Arakawa, Yuki Kawakami, Masumi Kimoto, Yoshitaka Takahashi, Toshiko Suzuki-Yamamoto.
Organizer
Okayama Prefectural University-Nanchang University Workshop for Food and Nutritional Sciences,
Place of Presentation
Okayama, Japan
Related Report
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[Presentation] Inhibitory effect of tea extracts on leukocyte-type 12-lipoxygenase activity.2014
Author(s)
Yuki Kawakami, Akemi Otsuki, Mai Kinoshita, Yoshiko Mori, Makiko Suzuki, Toshiko Suzuki-Yamamoto, Masumi Kimoto, Hideaki Tsuji, Hiromi Yamashita, Hideyuki Ito, Ding Zhi Fang, Yoshitaka Takahashi.
Organizer
Okayama Prefectural University-Nanchang University Workshop for Food and Nutritional Sciences,
Place of Presentation
Okayama, Japan
Related Report
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[Presentation] Inhibitory effect of Chinese tea on leukocyte-type 12-lipoxygenase activity. International Conference on Biologically Active Substances2012
Author(s)
Akemi Otsuki, Shiori Hirano, Mai Kinoshita, Ichika Shimoda, Yuki Kawakami, Toshiko Suzuki-Yamamoto, Hideaki Tsuji, Hideyuki Ito, Ding Zhi Fang and Yoshitaka Takahashi
Organizer
International Conference on Biologically Active Substances, Bioactive Okayam BAO2012
Place of Presentation
Okayama, Japan
Related Report
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