The investigation of the interaction between insulin sensitivity and fatty acids

• Project/Area Number: 24500994
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Eating habits, studies on eating habits
• Research Institution: Kyorin University (2014); Saitama Medical University (2012-2013)
• Principal Investigator: TOSHIO Hosaka 杏林大学, 医学部, 講師 (60403698)
• Research Collaborator: SAKAUE Hiroshi 徳島大学大学院, 栄養生命学教育部, 教授 (60372645)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: glut4 / トランスロケーション / 細胞骨格 / 脂肪酸 / GLUT4 / 脂肪合成

Outline of Final Research Achievements

Insulin stimulates GLUT4 vesicle recruitment from its intracellular storage site to the PM. Cysteine string protein 1 (CSP1) is a SNARE-binding protein involved in the vesicular trafficking of neurotransmitters and other exocytic processes. In this study, we found that CSP1 is involved in insulin resistance by interrupting GLUT4 vesicle docking with the PM.
The fatty acid binding protein X has reported to bind CSP1. The down-regulation of fatty acid binding protein X increases the CSP1 expression and decrease the insulin stimulated GLUT4 translocation. We are now investigating and confirming the preliminary result about the role of the fatty acid binding protein X in GLUT4 translocation and diabetes to exam the interaction with specific fatty acid and cytoskeleton and analyze the knockout mice, belonging to find the new targets of diabetes treatment.

Research Products

• [Journal Article] CSP1 modulates insulin sensitivity by attenuating GLUT4 vesicle docking with the plasma membrane (2013)
  • Author(s): Bayasgalan Jambaldorj, Eri Terada, Toshio Hosaka, Yuka Kishuku, Yukiko Tomioka, Kaori Iwashima, Yohko Hirata, Kiyoshi Teshigawara, Chung Thi Kim Le, Tadahiko Nakagawa, Nagakatsu Harada, Tohru Sakai, Hiroshi Sakaue, Toshio Matsumoto, Makoto Funaki, Akira Takahashi and Yutaka Nakaya1.
  • Journal Title: Journal of medical investigation Volume: 60(3-4) Pages: 197-204
  • Peer Reviewed
• [Journal Article] Activation of Akt through 5-HT2A receptor ameliorates serotonin-induced degradation of insulin receptor substrate-1 in adipocytes (2013)
  • Author(s): 李勤凱
  • Journal Title: Molecular and cellular endocrinology Volume: 365 Issue: 1 Pages: 25-35
  • DOI: 10.1016/j.mce.2012.08.022
  • Peer Reviewed
• [Journal Article] Decreased expression of MHC class II and cathepsin E in dendritic cells might contribute to impaired induction of antigen-specific T cell response in NC/Nga mice. (2012)
  • Author(s): Sakai T, Shuto E, Taki T, Imamura H, Kioka M, Nakamoto A, Mizoguchi S, Amano S, Kawano Y, Nakamoto M, Tsutumi R, Hosaka T.
  • Journal Title: Journal of medical investigation Volume: 59(1-2) Pages: 95-101
  • NAID: 130004465275
  • Peer Reviewed
• [Presentation] ATBP, Adipocyte-specific Tubulin-Binding Protein, Can Control Cytoskeletal Structure, and Insulin Stimulated Glucose Uptake (2015)
  • Author(s): Masashi Kuroda, Rie Okitsu, Mari Kondo, Kasumi Nakagawa, Toshio Hosaka, Nagakatsu Harada, Hiroshi Skaue
  • Organizer: 75th Scientific Sessions of American diabetes association
  • Place of Presentation: Boston, USA
  • Year and Date: 2015-06-05 – 2015-06-09
• [Presentation] 細胞骨格制御分子-synucleinによる糖取り込み機構の解析 (2014)
  • Author(s): 黒田雅史、阪上浩、保坂利男、興津理絵、原田永勝、中屋豊
  • Organizer: 日本肥満学会
  • Place of Presentation: 宮崎
  • Year and Date: 2014-10-24 – 2014-10-25
• [Presentation] 細胞骨格制御分子による糖取り込み機構の解析 (2014)
  • Author(s): 興津理絵、黒田雅士、保坂利男、坂東正浩、原田永勝、中屋豊、阪上浩
  • Organizer: 第57回日本糖尿病学会年次学術集会
  • Place of Presentation: 大阪
  • Year and Date: 2014-05-22 – 2014-05-24
• [Presentation] 細胞骨格制御分子による糖取り込み機構の解析 (2014)
  • Author(s): 興津理絵、阪上浩、保坂利男、高橋友奈、黒田雅士、Bayasgalan Jambaldorj、原田永勝、中屋豊
  • Organizer: 第87回日本内分泌学会
  • Place of Presentation: 福岡
• [Presentation] 細胞骨格制御分子による糖取り込み機構の解析 (2013)
  • Author(s): 興津理絵、阪上浩、保坂利男、高橋友奈、黒田雅士、Bayasgalan Jambaldorj、原田永勝、中屋豊
  • Organizer: 第34回日本肥満学会
  • Place of Presentation: 東京