(-)-Epigallocatechin-3-gallate increases the number of neural stem cells around the damaged area after rat traumatic brain injury

• Project/Area Number: 24501009
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Eating habits, studies on eating habits
• Research Institution: Kinki University
• Principal Investigator: SATOU Takao 近畿大学, 医学部附属病院, 教授 (70162443)
• Co-Investigator(Kenkyū-buntansha): ITOH Tatsuki 近畿大学, 農学部, 教授 (40330245) ; INOUE Takao 近畿大学, 医学部, 助教 (00441006)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 脳外傷 / 神経再生 / カテキン / 緑茶飲料 / 神経幹細胞 / 緑茶 / 神経再生促進 / 酸化ストレス / 神経保護作用

Outline of Final Research Achievements

A major component of green tea is (-)-epigallocatechin gallate (EGCG), which has strong antioxidant properties. we investigated the effect of EGCG on neural stem cell (NSC) proliferation following traumatic brain injury (TBI). Male Wistar rats that had access to normal drinking water, or water containing 0.1% (w/v) EGCG, ad libitum. Immunohistochemistry revealed that the number of nestin-positive cells after TBI in the EGCG group increased significantly. The number of single-stranded DNA (ssDNA)-positive cells after TBI significantly decreased in the EGCG group. Almost all ssDNA-positive cells in the water group co-localized with NeuN and nestin-staining. Spheres could only be isolated in the water group at 3 days.In the EGCG group, spheres could be isolated at all days following TBI. Spheres differentiated into neurons and glia. These results indicate that EGCG inhibits degradation of NSCs, which have the potential to differentiate into neurons and glia following TBI.

Research Products

• [Journal Article] By inhibiting Src, verapamil and dasatinib overcome multidrug resistance via increased expression of Bim and decreased expressions of MDR1 and survivin in human multidrug-resistant myeloma cells. (2014)
  • Author(s): Tsubaki M, Komai M, Itoh T, Imano M, Sakamoto K, Shimaoka H, Takeda T, Ogawa N, Mashimo K, Fujiwara D, Mukai J, Sakaguchi K, Satou T, Nishida S
  • Journal Title: Leuk Res Volume: 38 Issue: 1 Pages: 120-130
  • DOI: 10.1016/j.leukres.2013.10.017
  • Peer Reviewed
• [Journal Article] Cutaneous lymphangioma circumscriptum with marked blood presence: histopathologic evaluation of the endothelial cells (2014)
  • Author(s): Oiso N, Itoh T, Miyake M, Satou T, Kawada A
  • Journal Title: Eur J Dermatol Volume: 24 Issue: 1 Pages: 127-128
  • DOI: 10.1684/ejd.2013.2248
  • Peer Reviewed
• [Journal Article] Increased apoptotic neuronal cell death and cerebral dysfunction after traumatic brain injury in aged rats (2012)
  • Author(s): Itoh T, Imano M, Nishida S, Tsubaki M, Hashimoto S, Ito A, Satou T
  • Journal Title: Brain Struct Func Volume: (in press) Issue: 1 Pages: 209-220
  • DOI: 10.1007/s00429-012-0394-5
  • Peer Reviewed
• [Journal Article] (-)-Epigallocatechin-3-gallate increases the number of neural stem cells around the damaged area after rat traumatic brain injury (2012)
  • Author(s): Itoh T, Imano M, Nishida S, Tsubaki M, Hashimoto S, Ito A, Satou T
  • Journal Title: J Neural Transm Volume: (in press) Issue: 8 Pages: 877-890
  • DOI: 10.1007/s00702-011-0764-9
  • Peer Reviewed
• [Presentation] エピガロカテキンガレートの実験的脳外傷後に出現する神経幹細胞への影響 (2014)
  • Author(s): 佐藤隆夫、伊藤龍生
  • Organizer: 第103回日本病理学会総会
  • Place of Presentation: 広島国際会議場
  • Year and Date: 2014-04-24 – 2014-04-26
• [Presentation] 脳外傷前後の緑茶飲料による神経保護作用の検討 (2014)
  • Author(s): 伊藤龍生、佐藤隆夫
  • Organizer: 第103回日本病理学会総会
  • Place of Presentation: 広島国際会議場
  • Year and Date: 2014-04-24 – 2014-04-26