| Project/Area Number |
24501306
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Carcinogenesis
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| Research Institution | Juntendo University |
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Principal Investigator |
TABE Yoko 順天堂大学, 医学部, 准教授 (70306968)
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| Co-Investigator(Kenkyū-buntansha) |
IWABUCHI Kazuhisa 順天堂大学, 看護学部, 教授 (10184897)
SASAI Keisuke 順天堂大学, 医学部, 教授 (20225858)
金 林花 順天堂大学, 医学(系)研究科(研究院), 研究員 (90531955)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 低線量放射線 / 微小環境 / 前がん細胞 / microRNA / プロテオミクス / miRNA / 骨髄微小環境 / 間質細胞 / アポトーシス |
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| Outline of Final Research Achievements |
Exposure to low-dose ionizing radiation (LDIR) can alter mammalian cell gene expression. We compared the cellular response of irradiated immortalized, EB virus;infected, B-cells (EBV-B) cultured alone with that of EBV-B cells co-cultured with bone marrow derived stromal cells (MSCs). The miRNAs let7a, miR-15b, miR-16, miR-21 and a lipid metabolic miRNA hub miR-23b were upregulated after LDIR exposure in the mono-cultured EBV-B cells, but were downregulated in EBV-B cells co-irradiated with MSCs. A lipid biosynthesis enzyme GPAM, the common target of these miRNAs, was downregulated at the level of protein and mRNA expression in the LDIR exposed mono-cultured EBV-B cells and upregulated MSCs co-cultured EBV-B cells, suggesting a putative novel miRNA regulatory mechanism in the genetic control of the LDIR-induced stress response. These findings illustrate that LDIR exposure and the cell’s microenvironment can affect specific gene expression, resulting in altered protein expression.
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