Analysis of microRNA regulating malignant potential of mammary tumor in the bone microenvironment.
| Project/Area Number |
24501319
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Tumor biology
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| Research Institution | Nagoya City University |
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Principal Investigator |
FUTAKUCHI Mitsuru 名古屋市立大学, 医学(系)研究科(研究院), 准教授 (60275120)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 骨微小環境 / microRNA / がん幹細胞 / 微小環境 / 骨転移 / 動物モデル / 細胞増殖 |
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| Outline of Final Research Achievements |
We set the hypothesis that cancer cells arrived at the bone microenvironment (bone ME), would survive with phenotype similar to cancer stem cells. We found that miR205 was down-regulated in the bone ME. miR205 overexpressing tumor cell lines (miR205+cells) were established and involvement of EMT was found. We also found that the drug sensitivity of inhibitors of ER, AR and HER2 were enhanced in miR205+cells. IHC study of SOX2, one of the cancer stem cell marker, revealed that SOX2+ cells were observed in the bone ME and that the number of SOX2+ cells was significantly lower than that of control after trasplant of miR205+ cells. Cathepsin D was regulated by miR205 in the tumor cells and whosehigh expression were correlated with clinical stage and T grade of TNM classification. Taken together, miR205 may be involved in the survival, induction of EMT, and drug resistance of the tumor cells in the bone ME, all of which are malignant phenotypes of clinically defined bone metastasis.
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Report
(4 results)
Research Products
(43 results)
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[Journal Article] Murine Herc6 Plays a Critical Role in Protein ISGylation In Vivo and Has an ISGylation-Independent Function in Seminal Vesicles.2015
Author(s)
Arimoto KI, Hishiki T, Kiyonari H, Abe T, Cheng C, Yan M, Fan JB, Futakuchi M, Tsuda H, Murakami Y, Suzuki H, Zhang DE, Shimotohno K
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Journal Title
J Interferon Cytokine Res
Volume: 35
Pages: 351-358
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] Nanosized zinc oxide particles do not promote DHPN-induced lung carcinogenesis but cause reversible epithelial hyperplasia of terminal bronchioles.2014
Author(s)
Xu, J., Futakuchi, M., Alexander, D. B., Fukamachi, K., Numano, T., Suzui, M., Shimizu, H., Omori, T., Kanno, J., Hirose, A., and Tsuda, H.
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Journal Title
Arch Toxicol
Volume: 88
Pages: 65-75
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Size- and shape-dependent pleural translocation, deposition, fibrogenesis, and mesothelial proliferation by multiwalled carbon nanotubes.2014
Author(s)
Xu, J., Alexander, D. B., Futakuchi, M., Numano, T., Fukamachi, K., Suzui, M., Omori, T., Kanno, J., Hirose, A., and Tsuda, H.
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Journal Title
Cancer Sci
Volume: 105
Pages: 763-769
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Comparative study of toxic effects of anatase and rutile type nanosized titanium dioxide particles in vivo and in vitro.2014
Author(s)
Numano, T., Xu, J., Futakuchi, M., Fukamachi, K., Alexander, D. B., Furukawa, F., Kanno, J., Hirose, A., Tsuda, H., and Suzui, M.
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Journal Title
Asian Pacific J Cancer Prev
Volume: 15
Pages: 929-935
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] Inflammatory Activation after Experimental Cardiac Tamponade2013
Author(s)
Vass A, Süveges G, Erces D, Nógrády M, Varga G, Földesi I, Futakuchi M, Imai M, Okada N, Okada H, Boros M, Kaszaki J
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Journal Title
European surgical research.
Volume: 51
Pages: 1-13
DOI
Related Report
Peer Reviewed
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[Journal Article] Multi-walled carbon nanotubes translocate into the pleural cavity and induce visceral mesothelial proliferation in rats.2012
Author(s)
J. Xu, M. Futakuchi, H. Shimizu, D.B. Alexander, K. Yanagihara, K. Fukamachi, M. Suzui, J. Kanno, A. Hirose, A. Ogata, Y. Sakamoto, D. Nakae, T. Omori, H. Tsuda.
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Journal Title
Cancer Sci
Volume: 103
Pages: 2045-2050
DOI
Related Report
Peer Reviewed
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