The cell diversity and the cell lineage in Shh-type medulloblastoma.
Project/Area Number |
24501325
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Tumor biology
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Research Institution | Japanese Foundation for Cancer Research |
Principal Investigator |
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Project Period (FY) |
2012-04-01 – 2015-03-31
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Project Status |
Completed (Fiscal Year 2014)
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Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
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Keywords | 脳腫瘍 / 小脳髄芽腫 / miRNA / 癌幹細胞 / shh シグナル / Patched-1 / ニューロン分化 / 神経前駆細胞 / 神経分化 / shhシグナル / 髄芽腫 / 遺伝子発現制御 / Nmyc / ニューロン / 小脳腫瘍 / in vivo エレクトロポレーション / ノックアウトマウス |
Outline of Final Research Achievements |
Our immunohistochemical analyses and genetic lineage study showed that the medulloblastoma (MB) were composed of the MB cells of varied differentiation status, and that the cancer stem cells took the bipolar cell morphology and often differentiated into neurons with multiple dendrites like normal immature cerebellar granule neurons. We found that in MB, the expression patterns of miRNA-Xs that we have identified as the gatekeeper in MB development, were well correlated with the differentiation state of MB cells. Mis-expression of miRNA-X induced the differentiation of MB stem cell lines (in vitro), and the simultaneous inhibition of miRNA-Xs with Ptc1 cKO in granule cell progenitor cells induced the cancer stem cell-dominant MB (in vivo). These results indicate that miRNA-Xs must be involved in making the hierarchy of MB cells, promising their application to eradicate the cancer stem cells for MB cure.
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Report
(4 results)
Research Products
(13 results)
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[Journal Article] Assembly of the cochlear gap junction macromolecular complex requires connexin 26.2014
Author(s)
Kamiya K, Yum SW, Kurebayashi N, Muraki M, Ogawa K, Karasawa K, Miwa A, Guo X, Gotoh S, Sugitani Y, Yamanaka H, Ito-Kawashima S, Iizuka T, Sakurai T, Noda T, Minowa O, Ikeda K.
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Journal Title
J Clin Invest,
Volume: 124
Issue: 4
Pages: 1598-1607
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Assembly of the cochlear gap junction macromolecular complex requires connexin 26.2014
Author(s)
Kamiya K, Yum SW, Kurebayashi N, Muraki M, Ogawa K, Karasawa K, Miwa A, Guo X, Gotoh S, Sugitani Y, Yamanaka H, Ito-Kawashima S, Iizuka T, Sakurai T, Noda T, Minowa O, Ikeda K.
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Journal Title
J Clin Invest.
Volume: 124(4)
Pages: 1598-1607
Related Report
Peer Reviewed
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