Developments of A New Antibody Library and Antibody Chips
Project/Area Number |
24501343
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Tumor diagnosis
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Research Institution | Keio University |
Principal Investigator |
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Project Period (FY) |
2012-04-01 – 2015-03-31
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Project Status |
Completed (Fiscal Year 2014)
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Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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Keywords | EGFレセプター / 抗体 / ライブラリー / 抗体ライブラリー / IGFレセプター / アポトーシス / リン酸化 |
Outline of Final Research Achievements |
(1)We have analyzed effects on tumor cells of anti-EGF receptor antibodies recognized unique epitopes, which were obtained form our original antibody library. The antibodies induced apoptosis to EGFR-overproduced tumor cells with wild type and several tumorigenic K-RAS mutations. The antibodies inhibited EGF-induced EGFR phosphorylation and Akt phosphorylation. (2)For we have constructed an Fab antibody-displying phage library with 10^18 repertory which are based on new concept for library-screening system.
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Report
(4 results)
Research Products
(4 results)
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[Journal Article] Therapeutic Potential of Transgenic Mesenchymal Stem Cells Engineered to Mediate Anti-HMGB1 Activity: Targeting of Colon Cancer2014
Author(s)
Kikuchi, H., Yagi, H., Hasegawa, H., Ishii, Y., Okabayashi, K., Tsuruta, M., Hoshino, G., Takayanagi, A., Kitagawa, Y.
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Journal Title
Journal of Surgical Research
Volume: S0022-4804
Issue: 1
Pages: 00188-00197
DOI
Related Report
Peer Reviewed
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