• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

HSV-1 amplicon as a novel vector for gene transfer into refractory tumors

Research Project

Project/Area Number 24501351
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Clinical oncology
Research InstitutionNagoya University

Principal Investigator

GOSHIMA Fumi  名古屋大学, 医学(系)研究科(研究院), 助教 (70201499)

Co-Investigator(Kenkyū-buntansha) KIMURA Hiroshi  名古屋大学, 大学院医学系研究科, 教授 (30303621)
KAMAKURA Maki  名古屋大学, 医学(系)研究科(研究院), その他 (80437003)
Research Collaborator ESAKI Shinichi  名古屋市立大学, 大学院医学研究科, 助教 (20620983)
Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords遺伝子治療 / ウイルス / アンプリコン / 癌
Outline of Final Research Achievements

We made an HSV amplicon expressing murine GM-CSF to strengthen anti-tumor immune response for the treatment of ovarian cancer with intraperitoneal dissemination. Intraperitoneal injection of mGM-CSF amplicon prolonged survival and decreased intraperitoneal dissemination. Immunohistochemical staining revealed the infiltration of CD4- and CD8-positive cells into the peritoneal tumors. Murine splenic cells after each treatment were stimulated with HM-1 cells, and the strongest immune response was observed in the mice that received mGM-CSF amplicon injections. Now we have made an HSV amplicon expressing murine IL-2 and confirmed expression of IL2 in the infected cells.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (6 results)

All 2014 2013 2012

All Journal Article (3 results) (of which Peer Reviewed: 3 results,  Acknowledgement Compliant: 1 results) Presentation (3 results)

  • [Journal Article] Oncolytic viral therapy with a combination of HF10, a herpes simplex virus type 1 variant and granulocyte-macrophage colony-stimulating factor for murine ovarian cancer.2014

    • Author(s)
      Goshima F, Esaki S, Luo C, Kamakura M, Kimura H, Nishiyama Y.
    • Journal Title

      Int J Cancer.

      Volume: 134 Pages: 2865-2877

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Oncolytic viral therapy with a combination of HF10, a herpes simplex virus type 1 variant and granulocyte-macrophage colony-stimulating factor for murine ovarian cancer2014

    • Author(s)
      Goshima F1, Esaki S, Luo C, Kamakura M, Kimura H, Nishiyama Y
    • Journal Title

      Int J Cancer

      Volume: 15 Issue: 12 Pages: 2865-77

    • DOI

      10.1002/ijc.28631

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Enhanced antitumoral activitiy of oncolytic herpes simplex virus with gemcitabine using colorectal tumor models.2012

    • Author(s)
      Esaki S, Goshima F, Kimura H, Murakami S, Nishiyama Y.
    • Journal Title

      International Journal of Cancer

      Volume: 132 Issue: 7 Pages: 1592-601

    • DOI

      10.1002/ijc.27823

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Presentation] マウス悪性黒色腫両側皮下モデルにおけるダカルバジンの併用療法2014

    • Author(s)
      田中るい、五島典、江崎伸一、村田貴之、渡辺大輔、西山幸廣、木村宏
    • Organizer
      ウイルス学会
    • Place of Presentation
      パシフィコ横浜(神奈川県横浜市)
    • Year and Date
      2014-11-10 – 2014-11-12
    • Related Report
      2014 Annual Research Report
  • [Presentation] マウス卵巣癌腹腔播種モデルにおけるHSV-1 HF10とマウスGM-CSFアンプリコンを用いた抗腫瘍効果の検討2013

    • Author(s)
      五島典、江崎伸一、田中るい、村田貴之、渡辺大輔、木村宏、西山幸廣
    • Organizer
      第61回日本ウイルス学会学術集会
    • Place of Presentation
      神戸国際会議場(神戸市)
    • Related Report
      2013 Research-status Report
  • [Presentation] マウス卵巣癌腹膜播種モデルにおけるHSV-1 HF10とマウスGM-CSFアンプリコンを用いた治療効果の検討2012

    • Author(s)
      五島典、江崎伸一、武藤義文、岩田誠子、鎌倉真紀、渡辺大輔、木村宏、西山幸廣
    • Organizer
      日本ウイルス学会学術集会
    • Place of Presentation
      大阪(グランキューブ大阪)
    • Related Report
      2012 Research-status Report

URL: 

Published: 2013-05-31   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi