| Budget Amount *help |
¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
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| Outline of Final Research Achievements |
The radioadaptive response is the induction of cellular resistance to moderate or high doses of radiation by previous exposure to low doses. It has been suggested that the frequency of mis-rejoining of double-strand breaks (DSBs) is reduced in the radioadaptive response. There are two non-homologous end-joining (NHEJ) pathways to directly rejoin two ends of the breakpoint of DNA; classical NHEJ (C-NHEJ) and alternative NHEJ (alt-NHEJ). It is also known that DNA ligase IV is involved in C-NHEJ, and DNA ligase III in alt-NHEJ. In order to clarify which pathway is involved in the radioadaptive response, we investigated the radioadaptive response in the cells with reduced DNA ligase activity. We found that the radioadaptive response was suppressed in the cells with reduced amount of DNA ligase IV. On the contrary, the radioadaptive response was not suppressed in the cells with reduced amount of DNA ligase III. These results suggest that C-NHEJ is involved in the radioadaptive response.
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