Molecular analysis of the pathway of non-homologous end-joining involved in the radioadaptive response
| Project/Area Number |
24510064
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Risk sciences of radiation/Chemicals
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| Research Institution | Ibaraki University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
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| Keywords | 放射線適応応答 / 非相同末端結合 / DNAリガーゼ / 非相同末端結合修復 / DNA修復 / RNA干渉法 / 突然変異 |
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| Outline of Final Research Achievements |
The radioadaptive response is the induction of cellular resistance to moderate or high doses of radiation by previous exposure to low doses. It has been suggested that the frequency of mis-rejoining of double-strand breaks (DSBs) is reduced in the radioadaptive response. There are two non-homologous end-joining (NHEJ) pathways to directly rejoin two ends of the breakpoint of DNA; classical NHEJ (C-NHEJ) and alternative NHEJ (alt-NHEJ). It is also known that DNA ligase IV is involved in C-NHEJ, and DNA ligase III in alt-NHEJ. In order to clarify which pathway is involved in the radioadaptive response, we investigated the radioadaptive response in the cells with reduced DNA ligase activity. We found that the radioadaptive response was suppressed in the cells with reduced amount of DNA ligase IV. On the contrary, the radioadaptive response was not suppressed in the cells with reduced amount of DNA ligase III. These results suggest that C-NHEJ is involved in the radioadaptive response.
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Report
(4 results)
Research Products
(23 results)
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[Journal Article] Mutations in the FHA-domain of ectopically expressed NBS1 lead to Radiosensitization and to no increase in somatic mutation rates via a partial suppression of homologous recombination2014
Author(s)
Maki Ohara, Yumi Funyu, Shunsuke Ebara, Yuki Sakamoto, Ryota Seki, Kenta Iijima, Akiko Ohishi, Junya Kobayashi, Kenshi Komatsu, Akira Tachibana, and Hiroshi Tauchi
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Journal Title
J. Radiat. Res
Volume: 55
Issue: 4
Pages: 690-698
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Mitochondria-Targeted Superoxide Dismutase (SOD2) Regulates Radiation Resistance and Radiation Stress Response in HeLa Cells2012
Author(s)
Hosoki, A., S. Yonekura, Q-L. Zhao, Z-L. Wei, I. Takasaki, Y. Tabuchi, L-L. Wang, S. Hasuike, T. Nomura, A. Tachibana, K. Hashiguchi, S. Yonei, T. Kondo and Q-M. Zhang-Akiyama
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Journal Title
Journal of Radiation Research
Volume: 53
Issue: 1
Pages: 58-71
DOI
NAID
ISSN
0449-3060, 1349-9157
Related Report
Peer Reviewed
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[Presentation] Age dependent role of Ikaros and Pten mutations in radiation T-cell lymphomagenesis in mice.2013
Author(s)
Sunaoshi, M., Amasaki, Y., Hirano, S., Takabatake, T., Morioka, T., Nishimura, M., Shimada, Y., Tachibana, A., Kakinuma, S.
Organizer
Kyoto T Cell Conference
Place of Presentation
京都市
Related Report
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[Presentation] Age dependency of Ikaros and Pten alterations in radiation-induced T-cell lymphoma.2012
Author(s)
Sunaoshi, M., Amasaki, Y., Hirano, S., Takabatake, T., Morioka, T., Nishimura, M., Shimada, Y., Tachibana, A., Kakinuma, S.
Organizer
Childhood Cancer-2012; International Scientific Conference on Early Exposure and Childhood Cancer
Place of Presentation
Church House Conference Centre, London
Related Report
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