Biological impact Mechanism maintenance and assessment of biological impact of intracellular reactive oxygen species produced by normal physiological reaction
Project/Area Number |
24510072
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Risk sciences of radiation/Chemicals
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Research Institution | Kyoto University |
Principal Investigator |
Tano Keizo 京都大学, 原子炉実験所, 准教授 (00183468)
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Co-Investigator(Kenkyū-buntansha) |
MASUNAGA Shin-ichiro 京都大学, 原子炉実験所, 教授 (80238914)
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Project Period (FY) |
2012-04-01 – 2016-03-31
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Project Status |
Completed (Fiscal Year 2015)
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Budget Amount *help |
¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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Keywords | 活性酸素種 / 細胞内至適環境維持 / ラジカルスカベンジャー / 細胞内至適環境 / 国際情報交換、米国 |
Outline of Final Research Achievements |
Superoxide dismutases (SODs) are antioxidant proteins converting superoxide to hydrogen peroxide. In vertebrate cells, SOD1 is present in the cytoplasm, with small levels found in the nucleus and mitochondrial intermembrane space. SOD2 is present in the mitochondrial matrix. We conditionally disrupted the SOD1 or SOD2 gene in DT40 cells and found that depletion of SOD1 caused lethality, while depletion of SOD2 led to growth retardation. The lethality in SOD1-depleted cells was rescued by ascorbic acid. We demonstrated that ascorbic acid off set growth defects observed in SOD2-depleted cells and lowered mitochondrial superoxide to physiological levels in both SOD1- or SOD2-depleted cells. Depletion of SOD1 or SOD2 resulted in the accumulation of intracellular oxidative stress. This oxidative stress was reduced by ascorbic acid. Our study suggests that ascorbic acid can be applied as an antioxidant that mimics the functions of cytoplasmic and mitochondrial SODs.
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Report
(5 results)
Research Products
(33 results)
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[Journal Article] Effect of oxygen pressure during incubation with a 10B-carrier on 10B uptake capacity of cultured p53 wild-type and mutated tumor cells: dependency on p53 status of tumor cells and types of 10B-carriers2016
Author(s)
Masunaga S, Tatebe H, Nishimura Y, Tano K, Sanada Y, Moriwaki T, Sakurai Y, Tanaka H, Suzuki M, Kondo N, Maruhashi A and Ono K
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Journal Title
International Journal of Radiation Biology
Volume: 92
Issue: 4
Pages: 187-194
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] The effect of oxygen pressure during incubation with a 10B-carrier on 10B uptake capacity of cultured p53 wild-type and mutated tumor cells - Dependency on the p53 status of tumor cells and the type of 10B-carrier.2015
Author(s)
Masunaga S, Tatebe H, Nishimura Y, Tano K, Sanada Y, Moriwaki T, Sakurai Y, Tanaka H, Suzuki M, Kondo N, Maruhashi A and Ono K
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Journal Title
Oncol Rep
Volume: in press
Related Report
Peer Reviewed
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[Journal Article] Significance of Fractionated Administration of Thalidomide Combined With γ-Ray Irradiation in Terms of Local Tumor Response and Lung Metastasis.2014
Author(s)
Masunaga S, Sanada Y, Moriwaki T, Tano K, Sakurai Y, Tanaka H, SuzukiM, Kondo N, Narabayashi M, Watanabe T, Nakagawa Y, Maruhashi A and Ono K
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Journal Title
World J Oncol
Volume: 5
Pages: 155-165
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Protective roles of ascorbic acid in oxidative stress induced by depletion of superoxide dismutase in vertebrate cells2013
Author(s)
Tamari, Y., Nawata, H., Inoue, E., Yoshimura, A., Yoshii, H., Kashino, G., Seki, M., Enomoto, T., Watanabe, M., and Tano, K
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Journal Title
Free Rad. Res
Volume: 47
Issue: 1
Pages: 1-7
DOI
NAID
Related Report
Peer Reviewed
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[Presentation] Significance of Combined Treatment with Bevacizumab in Boron Neutron Capture Therapy in Terms of Local Tumor Response and Lung Metastasis.2014
Author(s)
Masunaga S, Sakurai Y, Tano K, Tanaka H, Suzuki M, Kondo N, Narabayashi3 M, Watanabe T, Nakagawa Y, Maruhashi A and Ono K
Organizer
16th International Congress on Neutron Capture Therapy
Place of Presentation
Helsinki, Finland
Year and Date
2014-06-14 – 2014-06-19
Related Report
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