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Bacterial methylmercury transporter MerE increases mercurials accumulation in Arabidopsis thaliana.

Research Project

Project/Area Number 24510104
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Environmental technology/Environmental materials
Research InstitutionKitasato University

Principal Investigator

KIYONO MASAKO  北里大学, 薬学部, 教授 (30239842)

Research Collaborator SONE Yuka  北里大学, 薬学部, 助教 (60550035)
Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Keywords環境修復技術 / ファイトレメディエーション / メチル水銀 / 水銀化合物 / トランスポーター / MerE / MerF / MerC
Outline of Final Research Achievements

The characteristics of bacteria take up mercury into cells via Mer superfamily which transport mercuric ions into the cytoplasm, have been applied in engineering of bioreactor used for mercurial bioremediation. We demonstrated that MerE is broad-spectrum mercury transporters that mediate both Hg(II), methylmercury and phenylmercury transport into bacterial cells. A transgenic Arabidopsis engineered to express MerE was constructed and the impact of expression of MerE on methylmercury accumulation was evaluated. The transgenic Arabidopsis expressing MerE accumulated significantly more methymercury and mercuric ions into plants than the wild-type Arabidopsis did. These results demonstrated that expression of the bacterial mercury transporter MerE promoted the transport and accumulation of methylmercury in transgenic Arabidopsis, which may be a useful method for improving plants to facilitate the phytoremediation.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (14 results)

All 2015 2014 2013 Other

All Journal Article (4 results) (of which Peer Reviewed: 4 results) Presentation (10 results)

  • [Journal Article] Mercurial-resistance determinants in Pseudomonas sp. K-62 plasmid pMR68.2013

    • Author(s)
      Sone Y, Mochizuki Y, Koizawa K, Nakamura R, Pan-Hou H, Itoh T, Kiyono M
    • Journal Title

      AMB express

      Volume: 3(1) Issue: 1 Pages: 41-41

    • DOI

      10.1186/2191-0855-3-41

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Role of MerC, MerE, MerF, MerT, and/or MerP in Resistance to Mercurials and the Transport of Mercurials in <i>Escherichia coli</i>2013

    • Author(s)
      Sone Y, Nakamura R, Pan-Hou H, Itoh T, Kiyono M
    • Journal Title

      Biological and Pharmaceutical Bulletin

      Volume: 36 Issue: 11 Pages: 1835-1841

    • DOI

      10.1248/bpb.b13-00554

    • NAID

      130003361552

    • ISSN
      0918-6158, 1347-5215
    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Increase methylmercury accumulation in Arabidopsis thaliana expressing bacterial broad-spectrum mercury transporter MerE.2013

    • Author(s)
      Sone Y, Nakamura R, Pan-Hou H, Sato MH, Itoh T, Kiyono M
    • Journal Title

      AMB express

      Volume: 3(1) Issue: 1 Pages: 52-52

    • DOI

      10.1186/2191-0855-3-52

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Bacterial heavy metal transporter MerC increases mercury accumulation in Arabidopsis thaliana2013

    • Author(s)
      Masako Kiyono et al.
    • Journal Title

      Biochemical Engineering Journal,

      Volume: 71 Pages: 19-24

    • DOI

      10.1016/j.bej.2012.11.007

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Presentation] 細菌由来の水銀トランスポーターMerC、MerE、MerF、MerTの機能解析2015

    • Author(s)
      曽根有香、中村亮介、高根沢康一、清野正子
    • Organizer
      本薬学会第135年会(神戸)
    • Place of Presentation
      神戸サンボーホールエリア他(神戸市)
    • Year and Date
      2015-03-28
    • Related Report
      2014 Annual Research Report
  • [Presentation] 水銀トランスポーターの水銀化合物に対する機能解析2014

    • Author(s)
      曽根有香、中村亮介、高根沢康一、清野正子
    • Organizer
      フォーラム2014 衛生薬学・環境トキシコロジー(つくば)
    • Place of Presentation
      つくば国際会議場(つくば市)
    • Year and Date
      2014-09-19
    • Related Report
      2014 Annual Research Report
  • [Presentation] MerCを利用したメチル水銀のファイトレメディエーション2013

    • Author(s)
      清野正子,曽根有香,中村亮介,伊藤智夫
    • Organizer
      フォーラム2013 衛生薬学・環境トキシコロジー
    • Place of Presentation
      福岡
    • Related Report
      2013 Research-status Report
  • [Presentation] メチル水銀トランスポーターを利用した水銀化合物のファイトレメディエーション2013

    • Author(s)
      清野正子,曽根有香
    • Organizer
      平成25年度メチル水銀研究ミーティング
    • Place of Presentation
      東京
    • Related Report
      2013 Research-status Report
  • [Presentation] 水銀耐性菌のメチル水銀トランスポーターに関する研究2013

    • Author(s)
      曽根有香、清野正子
    • Organizer
      平成25年度メチル水銀研究ミーティング
    • Place of Presentation
      東京
    • Related Report
      2013 Research-status Report
  • [Presentation] MerCおよびSNAREを利用した水銀・カドミウムのファイトレメディエーション

    • Author(s)
      清野正子、曽根有香、中村亮介、坂部貢、芳生秀光
    • Organizer
      第39回日本毒性学会学術年会
    • Place of Presentation
      仙台
    • Related Report
      2012 Research-status Report
  • [Presentation] Bacterial heavy metal transporter MerC increases mercury accumulation in Arabidopsis thaliana

    • Author(s)
      Masako Kiyono, Yuka Sone, Ryosuke Nakamura, Kou Sakabe, Hidemitsu Pan-Hou
    • Organizer
      The 6th International Congress of Asian Society of Toxicology
    • Place of Presentation
      仙台
    • Related Report
      2012 Research-status Report
  • [Presentation] Pseudomonas K-62 由来plasmid pMR68の水銀耐性遺伝子の機能解析

    • Author(s)
      清野正子,曽根有香,中村亮介,芳生秀光
    • Organizer
      フォーラム2012 衛生薬学・環境トキシコロジー
    • Place of Presentation
      名古屋
    • Related Report
      2012 Research-status Report
  • [Presentation] MerCおよびSNAREを利用した水銀のファイトレメディエーション

    • Author(s)
      清野正子、曽根有香、中村亮介、芳生秀光
    • Organizer
      日本薬学会第133年会
    • Place of Presentation
      横浜
    • Related Report
      2012 Research-status Report
  • [Presentation] 水銀トランスポーターMerC, MerE, MerF, MerTの機能解析

    • Author(s)
      曽根有香、中村亮介、芳生秀光、清野正子
    • Organizer
      日本薬学会第133年会
    • Place of Presentation
      横浜
    • Related Report
      2012 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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