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Structure-function studies of metabolic enzymes for design of novel biodegradable biopolymers

Research Project

Project/Area Number 24510114
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Environmental technology/Environmental materials
Research InstitutionThe Institute of Physical and Chemical Research

Principal Investigator

HISANO Tamao  独立行政法人理化学研究所, 放射光科学総合研究センター, 専任研究員 (20312267)

Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords環境調和型材料 / バイオポリエステル / 生分解性高分子 / 微生物酵素 / 構造と機能 / 結晶構造解析 / 生分解性
Outline of Final Research Achievements

Polyhydroxyalkanoate (PHA) is important bacterial polyester that is degraded by specific enzymes in environment. In this study, structure and function relationships of enzymes in PHA metabolism were investigated for the sake of development of the design and synthetic system of new biopolyesters. A multi-domain PHA depolymerase was crystallized, and its crystal structure in complex with an oligomeric substrate was successfully determined. The results indicated a novel interaction mode between the PHA depolymerase with the type I catalytic domain and polymer substrate. In addition, a novel hydrolase which degrades a soluble polymer polyaspartate was successfully crystallized, and its crystal structure in complex with an oligomeric substrate were determined. The interaction mode between the enzyme and the substrate indicated similarity with that of a fungal PHA depolymerase with the type II catalytic domain.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (8 results)

All 2014 Other

All Presentation (8 results) (of which Invited: 1 results)

  • [Presentation] ポリアスパラギン酸分解酵素の基質複合体の構造解析2014

    • Author(s)
      久野玉雄・平石知裕・南 皓介・桝田エリ子・阿部英喜・前田瑞夫・城 宜嗣
    • Organizer
      日本結晶学会平成26年度年会
    • Place of Presentation
      東京大学弥生キャンパス
    • Year and Date
      2014-11-01 – 2014-11-03
    • Related Report
      2014 Annual Research Report
  • [Presentation] ポリアスパラギン酸分解酵素の結晶構造2014

    • Author(s)
      久野玉雄・平石知裕・南 皓介・桝田エリ子・阿部英喜・前田瑞夫・城 宜嗣
    • Organizer
      第63回高分子討論会
    • Place of Presentation
      長崎大学文教キャンパス
    • Year and Date
      2014-09-25
    • Related Report
      2014 Annual Research Report
  • [Presentation] Enzymatic hydrolysis of the β-β amide linkage in poly-β-aspartate2014

    • Author(s)
      Tamao Hisano, Tomohiro Hiraishi, Kosuke Minami, Eriko Masuda, Hideki Abe, Mizuo Maeda, Yoshitsugu Shiro
    • Organizer
      23rd Congress and General Assembly of the International Union of Crystallography
    • Place of Presentation
      Montreal, Canada
    • Year and Date
      2014-08-06 – 2014-08-08
    • Related Report
      2014 Annual Research Report
  • [Presentation] ポリアスパラギン酸分解酵素の結晶構造

    • Author(s)
      久野玉雄、平石知裕、南皓介、桝田エリ子、阿部英喜、前田瑞夫、城宜嗣
    • Organizer
      第13回日本蛋白質科学会年会
    • Place of Presentation
      とりぎん会館、鳥取
    • Related Report
      2013 Research-status Report
  • [Presentation] Crystal structure of poly-b-aspartate hydrolase from Pedobactor sp. KP-2

    • Author(s)
      Tamao Hisano, Tomohiro Hiraishi, Kosuke Minami, Eriko Masuda, Hideki Abe, Mizuo Maeda, Yoshitsugu Shiro
    • Organizer
      The 4th International Conference on Biobased Polymers (ICBP2013)
    • Place of Presentation
      漢陽大学校, Seoul, Korea
    • Related Report
      2013 Research-status Report
  • [Presentation] ポリアスパラギン酸分解酵素の結晶構造解析

    • Author(s)
      久野玉雄、平石知裕、南皓介、桝田エリ子、阿部英喜、前田瑞夫、城宜嗣
    • Organizer
      平成25年度日本結晶学会年会
    • Place of Presentation
      熊本大学、熊本
    • Related Report
      2013 Research-status Report
  • [Presentation] ポリ-β-アスパラギン酸分解酵素とオリゴマー基質との複合体の結晶構造

    • Author(s)
      久野玉雄、平石知裕、南皓介、桝田エリ子、阿部英喜、前田瑞夫、城宜嗣
    • Organizer
      第36回日本分子生物学会年会
    • Place of Presentation
      神戸ポートアイランド、神戸
    • Related Report
      2013 Research-status Report
  • [Presentation] Introduction to Protein Crystallography

    • Author(s)
      Tamao Hisano, Tomohiro Hiraishi, Kosuke Minami, Eriko Masuda, Hideki Abe, Mizuo Maeda, Yoshitsugu Shiro
    • Organizer
      Introduction to Protein Crystallography A Hands-On Workshop
    • Place of Presentation
      Universiti Sains Malaysia, Penang, Malaysia
    • Related Report
      2013 Research-status Report
    • Invited

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Published: 2013-05-31   Modified: 2019-07-29  

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