| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
|
|---|
| Outline of Final Research Achievements |
In this study, I developed new data reduction method in order to obtain unique and trailblazing information of proton positions in protein structures. I have succeeded two integration method (optimization method of TOF binning and optimization method of 3-dimensional region of diffraction spot for the improvement of accuracy of the TOF diffraction data. I tried to apply these method to the TOF diffraction data set measured by IBARAKI biological crystal diffractometer. In comparison to the results by old method, data statistic and reliability of the structure model were improved. It was demonstrated that these method was useful. From the result of the structure analysis with the data obtained by new method, I could observed protonation states of active site and a water molecule with D atom which is hydrogen bonded to active site. It is assumed that the water contribute to reaction mechanism.
|
