Development of 19F-NMR-Based Multiplex Focused Gene Diagnostic Systems
Project/Area Number |
24510165
|
Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Nanomaterials/Nanobioscience
|
Research Institution | University of Tsukuba |
Principal Investigator |
OISHI Motoi 筑波大学, 数理物質系, 講師 (90419242)
|
Project Period (FY) |
2012-04-01 – 2015-03-31
|
Project Status |
Completed (Fiscal Year 2014)
|
Budget Amount *help |
¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2014: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
|
Keywords | 19F-NMR / DNA / 磁性粒子 / 金ナノ粒子 / 19F-NMR / DNA / センサー / 酵素 |
Outline of Final Research Achievements |
An ultrasensitive colorimetric assay for both DNA and RNA has been developed utilizing enzyme-free click chemical ligation chain reaction (CCLCR) on dispersed gold nanoparticles and magnetic beads. The CCLCR assay provides ultrasensitive detection (50 zM = 10-21 M: several copies) of target DNA that is comparable to PCR-based approaches. Note that target RNA could also be detected with similar sensitivity without the need for reverse transcription to the corresponding cDNA. Thus, this method might be a useful tool for development of 19F-NMR–based multiplex focused gene diagnostic systems.
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Report
(4 results)
Research Products
(11 results)