Bioinformatic and molecular biological study on ultra-short introns
Project/Area Number |
24510271
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Genome biology
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Research Institution | Fujita Health University |
Principal Investigator |
SHIMADA Makoto 藤田保健衛生大学, 総合医科学研究所, 講師 (00528044)
|
Co-Investigator(Renkei-kenkyūsha) |
MAYEDA Akila 藤田保健衛生大学, 総合医科学研究所, 教授 (50212204)
|
Research Collaborator |
SASAKI Noriko (HARAGUCHI Noriko) 藤田保健衛生大学, 総合医科学研究所, 博士研究員 (90546649)
|
Project Period (FY) |
2012-04-01 – 2016-03-31
|
Project Status |
Completed (Fiscal Year 2015)
|
Budget Amount *help |
¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2015: ¥130,000 (Direct Cost: ¥100,000、Indirect Cost: ¥30,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
|
Keywords | イントロン / スプライシング / 進化 / 遺伝子発現 / 脳 / 選択的スプライシング / 制御機構 / 人類進化 / データーベース / 突然変異 / 潜在的スプライシング部位 / ヒト遺伝子 / データベース |
Outline of Final Research Achievements |
We found introns that are shorter than previously expected and published them as “ultra-short introns.” Because exon/intron boundary is dynamically defined, we evaluated the ultra-short introns using transcript and genome sequences of vertebrate species as well as human. We found seven orthologous ultra-short introns in experimentally validated transcript sequences. In case of other ultra-short introns, few transcript sequences are available in non-human species. Moreover, splice di-nucleotides sequences of these ultra-short introns scarcely conserved in genome sequences. These results suggest that at least some of ultra-short introns have been appeared within human or closely related species and/or are specifically expressed depending on cell types or development stages.
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Report
(5 results)
Research Products
(17 results)