Development of aging markers for screening for genes regulating chronological aging
| Project/Area Number |
24510274
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Genome biology
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| Research Institution | The Institute of Physical and Chemical Research |
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Principal Investigator |
MATSUYAMA Akihisa 独立行政法人理化学研究所, 吉田化学遺伝学研究室, 専任研究員 (90399444)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 経時老化 / リバースプロテオミクス / リバースプロテオクミス |
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| Outline of Final Research Achievements |
Chronological lifespan (CLS) is defined as the length of time a cell can survive without cell division, which may serve as a good model for studying the mechanisms regulating life span of differentiated cells that are generally non-dividing. Chronological age of cells has been estimated by measuring colony forming units of cells in stationary phase. However, this method is a very time-consuming and daunting task. To address this issue, we tried to develop molecular markers that can provide an indirect measure of chronological age of a cell using fission yeast. Using the strain collection, a set of strains that can express each of the fission yeast ORFs with small epitope tags, we identified proteins whose expression levels were altered according to the time in stationary phase. Tagging of these candidates with fluorescent proteins allows immediate measuring of cell age from the fluorescence intensity. These aging markers will help identify factors regulating CLS.
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Report
(4 results)
Research Products
(11 results)
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[Journal Article] 1.SUMOylation regulates telomere length by targeting the shelterin subunit Tpz1/Tpp1 to modulate shelterin-Stn1 interaction in fission yeast.2014
Author(s)
Miyagawa K, Low RS, Santosa V, Tsuji H, Moser BA, Fujisawa S, Harland JL, Raguimova ON, Go A, Ueno M, Matsuyama A, Yoshida M, Nakamura TM, Tanaka K
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Journal Title
Proceedings of the National Academy of Sciences, U S A.
Volume: 111
Pages: 5950-5955
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Cross-species protein interactome mapping reveals species-specific wiring of stress response pathways.2013
Author(s)
Jishnu Das, Tommy V. Vo, Xiaomu Wei, Joseph C. Mellor, Virginia Tong, Andrew G. Degatano, Xiujuan Wang, Lihua Wang, Nicolas A. Cordero, Nathan Kruer-Zerhusen, Akihisa Matsuyama, Jeffrey A. Pleiss, Steven M. Lipkin, Minoru Yoshida, Frederick P. Roth, and Haiyuan Yu
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Journal Title
Science Signaling
Volume: 6
DOI
Related Report
Peer Reviewed
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