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Development of aging markers for screening for genes regulating chronological aging

Research Project

Project/Area Number 24510274
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Genome biology
Research InstitutionThe Institute of Physical and Chemical Research

Principal Investigator

MATSUYAMA Akihisa  独立行政法人理化学研究所, 吉田化学遺伝学研究室, 専任研究員 (90399444)

Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords経時老化 / リバースプロテオミクス / リバースプロテオクミス
Outline of Final Research Achievements

Chronological lifespan (CLS) is defined as the length of time a cell can survive without cell division, which may serve as a good model for studying the mechanisms regulating life span of differentiated cells that are generally non-dividing. Chronological age of cells has been estimated by measuring colony forming units of cells in stationary phase. However, this method is a very time-consuming and daunting task. To address this issue, we tried to develop molecular markers that can provide an indirect measure of chronological age of a cell using fission yeast. Using the strain collection, a set of strains that can express each of the fission yeast ORFs with small epitope tags, we identified proteins whose expression levels were altered according to the time in stationary phase. Tagging of these candidates with fluorescent proteins allows immediate measuring of cell age from the fluorescence intensity. These aging markers will help identify factors regulating CLS.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (11 results)

All 2014 2013 2012 Other

All Journal Article (4 results) (of which Peer Reviewed: 3 results,  Open Access: 1 results,  Acknowledgement Compliant: 1 results) Presentation (3 results) (of which Invited: 2 results) Remarks (4 results)

  • [Journal Article] 1.SUMOylation regulates telomere length by targeting the shelterin subunit Tpz1/Tpp1 to modulate shelterin-Stn1 interaction in fission yeast.2014

    • Author(s)
      Miyagawa K, Low RS, Santosa V, Tsuji H, Moser BA, Fujisawa S, Harland JL, Raguimova ON, Go A, Ueno M, Matsuyama A, Yoshida M, Nakamura TM, Tanaka K
    • Journal Title

      Proceedings of the National Academy of Sciences, U S A.

      Volume: 111 Issue: 16 Pages: 5950-5955

    • DOI

      10.1073/pnas.1401359111

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] 全タンパク質の電気泳動度データベース:Mobilitome2014

    • Author(s)
      松山 晃久、白井 温子、吉田 稔
    • Journal Title

      生物物理化学 電気泳動

      Volume: 58 Pages: 89-91

    • NAID

      130004704698

    • Related Report
      2014 Annual Research Report
  • [Journal Article] Cross-species protein interactome mapping reveals species-specific wiring of stress response pathways.2013

    • Author(s)
      Jishnu Das, Tommy V. Vo, Xiaomu Wei, Joseph C. Mellor, Virginia Tong, Andrew G. Degatano, Xiujuan Wang, Lihua Wang, Nicolas A. Cordero, Nathan Kruer-Zerhusen, Akihisa Matsuyama, Jeffrey A. Pleiss, Steven M. Lipkin, Minoru Yoshida, Frederick P. Roth, and Haiyuan Yu
    • Journal Title

      Science Signaling

      Volume: 6 Issue: 276

    • DOI

      10.1126/scisignal.2003350

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Determining proteome-wide expression levels using reverse protein arrays in fission yeast.2012

    • Author(s)
      Bauer F, Matsuyama A, Yoshida M, Hermand D.
    • Journal Title

      Nature Protocols

      Volume: 7 Issue: 10 Pages: 1830-5

    • DOI

      10.1038/nprot.2012.114

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Presentation] 全タンパク質の電気泳動度データベース:Mobilitome2014

    • Author(s)
      松山 晃久、白井 温子、吉田 稔
    • Organizer
      第65回 日本電気泳動学会総会 シンポジウム
    • Place of Presentation
      横浜情報文化センター
    • Year and Date
      2014-10-25
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Reverse proteomics in fission yeast.2012

    • Author(s)
      Matsuyama A, Yoshida, M.
    • Organizer
      The Second Annual Meeting for Whole-Organism Science Society
    • Place of Presentation
      RIKEN SPring-8 Center, Harima Institute
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] eIF5Aによる発現制御を受けるタンパク質の網羅的探索

    • Author(s)
      近藤直子、白井温子、松山晃久、夏目徹、浜本牧子、吉田稔
    • Organizer
      2014年度農芸化学会大会
    • Place of Presentation
      神奈川県川崎市・明治大学生田キャンパス
    • Related Report
      2013 Research-status Report
  • [Remarks] How yeast responds to change

    • URL

      http://www.riken.jp/en/research/rikenresearch/highlights/7169

    • Related Report
      2013 Research-status Report
  • [Remarks] 老化に関わる遺伝子を網羅的に探し出す

    • URL

      http://www.asi.riken.jp/jp/publications/leaflet/number3/matsuyama/index.html

    • Related Report
      2013 Research-status Report
  • [Remarks] How yeast responds to change

    • URL

      http://www.rikenresearch.riken.jp/eng/research/7169.html

    • Related Report
      2012 Research-status Report
  • [Remarks] 老化に関わる遺伝子を網羅的に探し出す

    • URL

      http://www.asi.riken.jp/jp/publications/leaflet/number3/matsuyama/index.html

    • Related Report
      2012 Research-status Report

URL: 

Published: 2013-05-31   Modified: 2019-07-29  

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