Mechanism of lifespan regulation in diploid cells of the budding yeast Saccharomyces cerevisiae as a model for cellular lifespan study
Project/Area Number |
24510279
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Medical genome science
|
Research Institution | Nagahama Institute of Bio-Science and Technology |
Principal Investigator |
MUKAI Yukio 長浜バイオ大学, バイオサイエンス学部, 准教授 (60252615)
|
Project Period (FY) |
2012-04-01 – 2015-03-31
|
Project Status |
Completed (Fiscal Year 2014)
|
Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
|
Keywords | 寿命 / 二倍体細胞 / 出芽酵母 / 老化疾患モデル / トランスクリプトーム / メタボローム / 必須遺伝子 / 酵母 / トランスクリプトーム解析 / メタボローム解析 |
Outline of Final Research Achievements |
The budding yeast Saccharomyces cerevisiae is one of the most important model organisms for studying cellular senescence and lifespan. Haploid cells of the yeast is convenient for analyzing gene function. In this study, we have investigated yeast diploid cells as a better model for cellular lifespan study since higher organisms are generally diploid cells. Diploid cells of wild-type strain exhibited longer replicative lifespan, the number of cells produced from a cell before dying, than haploid cells. During searching genes that are specifically expressed in diploid cells, we found novel longevity genes. Using heterozygous diploid knockout mutants, we discovered novel longevity genes essential for cell proliferation.
|
Report
(4 results)
Research Products
(14 results)