Basic studies on activities, action mechanism and application of a new anti-oomycota antibiotic
| Project/Area Number |
24510309
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Living organism molecular science
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| Research Institution | Ritsumeikan University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
KAWASAKI Takashi 立命館大学, 薬学部, 助教 (00408733)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
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| Keywords | 生理活性物質 / 結合タンパク質 / 生合成遺伝子 / サプロマイシン |
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| Outline of Final Research Achievements |
Oomycota infect plants and animals and cause severe damage in some situation. Compound 046-E produced by microorganism possess selective activity against oomycota, therefore, it seemed to become a useful preventive agent. We investigated the target molecule of 046-E and identified nucleoside diphosphate kinase B as a binding protein, however, its enzymatic activity was not inhibited by 046-E. We also studied on effects of 046-E to aquatic plankton and preventive effects against oomycota infection on fish eggs, and it was revealed that 046-E is harmless to aquatic plankton and possess potent preventive activity against oomycota infection on fish eggs at low concentration. We attempted to clone and identify a biosynthetic gene cluster for an angucycline-type antibiotics 046-E from Streptomyces sp. TK08046. Finally, we found that a gene cluster involved in 046-E biosynthesis.
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Report
(4 results)
Research Products
(5 results)
