| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Outline of Final Research Achievements |
This study performed nanoscale analysis of natural nanoparticles that ubiquitously occur on the Earth surface by means of high resolution transmission electron microscopy in conjunction with a variety of bulk analytical techniques. In Fe and Ce oxide nanoparticles, the surface atoms revealed lower coordination number than bulk. The relationship of nanoparticle with environmental nanoparticles was also investigated. After interaction of microorganisms with inert nanoparticles, the microbial metabolisms were slightly affected; The relative composition of the extracellular polymeric substances (EPS) has been changed as revealed by high performance liquid chromatography. Peptide mass finger print analysis of the intracellular substances exhibited an expression of Eno2p, which is involved in glycolysis of metabolism. The impact of EPS on nanoparticle agglomeration was also evaluated and the adsorption of EPS formed steric barrier resulting in stabilization of colloids.
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