Hidden electronic excited state of enhanced green fluorescent protein
| Project/Area Number |
24550033
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Physical chemistry
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| Research Institution | Toho University |
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Principal Investigator |
HOSOI Haruko 東邦大学, 理学部, 講師 (00313396)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | マルチプレックス二光子吸収分光法 / HBDI / eGFP / 電子励起S2状態 / 高振動励起S1状態 / 蛍光タンパク質 / 二光子蛍光メカニズム / 発色団モデル化合物 |
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| Outline of Final Research Achievements |
Fluorescence imaging by two-photon excitation has attracted attention as a new imaging technique. However, fluorescence mechanism of two-photon excited fluorescent proteins is completely unknown. We measured two-photon absorption spectra of enhanced GFP (eGFP), which is the most important marker, and its model chromophore, HBDI, in various solvents by a multiplex two-photon absorption spectroscopy to clarify the fluorescence mechanism. The results indicate that the existence of a "hidden" excited state in the vicinity of the lowest excited singlet state of eGFP. We conclude that this is the origin of the discrepancy between the one-photon and two-photon excitation spectra of eGFP, which is well known in the field of biology.
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Report
(4 results)
Research Products
(5 results)
