| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Outline of Final Research Achievements |
By using preferential enzyme-inhibitor interactions, we study the photophysical properties of artificial Carbonic Anhydrase or Chymotrypsin-[Ru(bpy)3]2+ type complex and the detailed photoinduced electron- or energy-transfer mechanisms are discussed. For example, we prepared a Ru(II)-based CA inhibitor,tethering a benzenesulfonamide group and a [Ru(bpy)3]2+ moiety and constructed the artificial CA-Ru(II) complex. After steady-state photoirradiation, the photo-excited triplet state of Ru(II)* was quenched by a sacrificial quencher through an intermolecular photoinduced ET mechanism, giving the oxidized Ru(III) complex. The following intramolecular electron abstraction from an amino-acid residue near the active site of CA proceeded.
On the other hand, several types of the [Ru(bpy)2(L)]2+ complexes were synthesized and their electrochemical and spectral properties were examined. Spin densities were analyzed to characterize triplet states in those complexes.
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