An assessment study on the chromatography combined with small-angle X-ray scattering technique for characterization of biological macromolecules
Project/Area Number |
24550111
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Analytical chemistry
|
Research Institution | National Agriculture and Food Research Organization |
Principal Investigator |
WATANABE Yasushi 独立行政法人農業・食品産業技術総合研究機構, 食品総合研究所食品バイオテクノロジー研究領域, 上席研究員 (30353957)
|
Project Period (FY) |
2012-04-01 – 2015-03-31
|
Project Status |
Completed (Fiscal Year 2014)
|
Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
|
Keywords | 分離分析 / 食品関連生体高分子 / 分離直後の溶液構造物性評価 / クロマトグラフィー / 生体高分子 / 溶液構造物性評価 |
Outline of Final Research Achievements |
In this study, to present the appropriate application of the size-exclusion chromatography combined with small-angle X-ray scattering technique, we will evaluate this measurement system by characterization of biological macromolecules, such as a large glycoprotein proteoglycan. This method will be useful for the estimation of the size (the radius of gyration value), zero-angle scattering intensity (molecular weight) and structural properties (conformations or persistence lengths) of separated biological macromolecules with dimensions of 1-10 nm in solution. The present results show that the combination of the small-angle X-ray and light scattering methods will be a powerful tool for characterization of large biopolymers in solution. Since the potential of this technique depends on the performance of the X-ray scattering instrument, further development of the measurement system will be important for future food and biological sciences.
|
Report
(4 results)
Research Products
(11 results)