Elucidation of antiviral activity of sulfated polysacharides by SPR
Project/Area Number |
24550129
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Polymer chemistry
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Research Institution | Kitami Institute of Technology |
Principal Investigator |
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Co-Investigator(Kenkyū-buntansha) |
NAKASHIMA Hideki 聖マリアンナ医科大学, 医学部, 教授 (20192669)
|
Project Period (FY) |
2012-04-01 – 2015-03-31
|
Project Status |
Completed (Fiscal Year 2014)
|
Budget Amount *help |
¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
|
Keywords | 硫酸化糖鎖 / 抗ウイルス性 / 構造解析 / 生理活性 / 開環重合 / 天然糖鎖 / 静電的相互作用 / モデルペプチド / 表面プラズモン共鳴 / 高分解能NMR / ペプチド / 相互作用 / SPR / NMR / 糖鎖 |
Outline of Final Research Achievements |
For the elucidation of the structure-biological activity relationship, the synthesis of galactomannans by ring-opening copolymerization of 1, 6-anhydro-tri-O-benzyl-beta-D-mannopyranose (LMTBE) and 1, 6-anhydro-2, 3-di-O-benzyl-4-O-(2', 3', 4', 6'-tetra-O-benzyl-alpha-D-galactopyranosyl)-beta-D-mannopyranose (LMGABE) was carried out by PF5 as a catalyst under high vacuum at -60°C. The synthetic galactomannans were obtained in good yields. After debenzylation with Na in liquid NH3 to recover hydroxyl groups, the resulting synthetic galactomannans were sulfated by piperidine N-sulfonic acid in DMSO to give sulfated galactomannans with high anti-HIV activity and low cytotoxicity. The anti-HIV mechanism of sulfated synthetic galactomannans was estimated by using SPR, DSL, and zeta potential measurements, suggesting that the electrostatic interaction between negatively charged sulfate groups and positively charged amino groups was an important role in the biological mechanism.
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Report
(4 results)
Research Products
(82 results)
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[Journal Article] Sulfated polysaccharide, curdlan sulfate, efficiently prevents entry/fusion and restricts antibody-dependent enhancement of dengue virus infection in vitro: A possible candidate for clinical application2013
Author(s)
Koji Ichiyama, Sindhoora Bhargavi Gopala Reddy, Li Feng Zhang, Wei Xin Chin, Tegshi Muschin, Lars Heinig, Youichi Suzuki, Haraprasad Nanjundappa, Yoshiyuki Yoshinaka, Akihide Ryo, Nobuo Nomura, Eng Eong Ooi, Subhash G. Vasudevan, Takashi Yoshida, Naoki Yamamoto
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Journal Title
PLOS Neglected Tropical Diseases
Volume: 7
Related Report
Peer Reviewed
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