Conformational dynamics of non-B DNA in vivo
Project/Area Number |
24550188
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Chemistry related to living body
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Research Institution | Osaka University |
Principal Investigator |
CHOI Jungkweon 大阪大学, 産業科学研究所, 助教 (00574328)
|
Project Period (FY) |
2012-04-01 – 2015-03-31
|
Project Status |
Completed (Fiscal Year 2014)
|
Budget Amount *help |
¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
|
Keywords | Non-B DNA / i-motif / G-quadruplex / Charge transfer / Folding / RecA protein / FRET / i-motif DNA / Electron Transfer / G-quadruple / 電荷分離 |
Outline of Final Research Achievements |
The charge transfer in i-motif and G-quadruplex DNA, which have a tetraplex structure, has been investigated by various time-resolved spectroscopies. The result showed that i-motif and G-quadruplex DNA can act as a good electron carrier or hole captor in DNA-based electronic devices. Meanwhile, the RecA@ssDNA filament, which is formed by the interaction between RecA protein and G-rich sequence, was dissociated by the addition of K+ ions and the dissociated G-rich sequence was quickly folded to G-quadruplex structure. The conformation of G-quadruplex is converged to the specific G-quadruplex with one double-chain-reversal loop upon association of RecA protein. Furthermore, I found that the folding reaction of G-rich sequence (37htel) may undergo with two-state mechanism without any detectable intermediate and that the global structureis still occurring even after the formation of a secondary structure.
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Report
(4 results)
Research Products
(18 results)