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Construction of the tumor marker saccharide targeting artificial receptors and characterization of their membrane fusion activities

Research Project

Project/Area Number 24550196
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Chemistry related to living body
Research InstitutionNihon University

Principal Investigator

KASHIWADA Ayumi  日本大学, 生産工学部, 准教授 (80350031)

Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2014: ¥260,000 (Direct Cost: ¥200,000、Indirect Cost: ¥60,000)
Fiscal Year 2013: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywords腫瘍細胞マーカー / TF二糖構造 / 糖鎖認識 / リポソーム / 膜融合 / 薬物送達系 / 二糖認識 / ボロン酸誘導体 / 薬物送達 / 糖鎖
Outline of Final Research Achievements

We designed and synthesized a novel receptor targeted against the Gal-beta-1,3-GalNAc disaccharide incorporated liposomal vesicles. We selected a peptidyl bis(boroxole) derivative as the disaccharide recognition moiety of the designed receptor. We have previously constructed target-selective liposomal membrane fusion systems. To develop the functional liposomes with targeting capabilities against the Gal-beta-1,3-GalNAc disaccharide, we prepared the receptor incorporated liposomes and some types of the glyco- or glycosphingo- lipids possessing target liposomes. The membrane fusion behavior was examined by a probe dilution assay based on the fluorescence resonance energy transfer (FRET) between the membrane-bound fluorophores NBD-PE and Rh-PE. The fusion kinetics based on the lipid mixing indicates thatthe designed receptor acts as an effective membrane fusion device toward Gal-beta-1,3-GalNAc disaccharide.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (5 results)

All 2015 2014 2013 2012

All Presentation (5 results) (of which Invited: 1 results)

  • [Presentation] pH Dependence of Disruption of Liposomal Membranes by Artificial Lytic Peptide2015

    • Author(s)
      Ayumi Kashiwada, Masaki Mizuno, Iori Yamane, Jun-ichi Hashimoto
    • Organizer
      27th European Conference on Biomaterials
    • Place of Presentation
      ICE Conference venue, Kraków / Poland
    • Year and Date
      2015-08-30 – 2015-09-03
    • Related Report
      2014 Annual Research Report
  • [Presentation] Synthesis and Characterization of Novel pH-Triggered Membrane Lytic Peptides2014

    • Author(s)
      Ayumi Kashiwada
    • Organizer
      人工光合成ワークショップ:ペプチドおよびタンパク質科学に基づく光収穫プロセス工学
    • Place of Presentation
      名古屋工業大学
    • Year and Date
      2014-10-06
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Disruption of Liposomes by pH-Dependent Membrane Lytic Peptides2014

    • Author(s)
      Ayumi Kashiwada, Jun-ichi Hashimoto, Masaki Mizuno
    • Organizer
      5th EuCheMS Chemistry Congress
    • Place of Presentation
      WOW Istanbul Convention Center, Istanbul / Turkey
    • Year and Date
      2014-08-31 – 2014-09-04
    • Related Report
      2014 Annual Research Report
  • [Presentation] Thomsen-Friedenreich (TF) 二糖選択的な膜融合系の構築2013

    • Author(s)
      鯉沼聖美, 大森真乗, 中島千織, 柏田 歩
    • Organizer
      第7回バイオ関連化学シンポジウム
    • Place of Presentation
      名古屋大学東山キャンパス
    • Related Report
      2013 Research-status Report
  • [Presentation] Construction of high-preformance artificial membrane fusion system with target-selectivity2012

    • Author(s)
      Ayumi Kashiwada, Mana Tsuboi, Iori Yamane, Kiyomi Matsuda
    • Organizer
      4th EuCheMS Chemistry Congress
    • Place of Presentation
      Prague, Czech Republic
    • Related Report
      2012 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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