Construction of the tumor marker saccharide targeting artificial receptors and characterization of their membrane fusion activities
| Project/Area Number |
24550196
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Chemistry related to living body
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| Research Institution | Nihon University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2014: ¥260,000 (Direct Cost: ¥200,000、Indirect Cost: ¥60,000)
Fiscal Year 2013: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 腫瘍細胞マーカー / TF二糖構造 / 糖鎖認識 / リポソーム / 膜融合 / 薬物送達系 / 二糖認識 / ボロン酸誘導体 / 薬物送達 / 糖鎖 |
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| Outline of Final Research Achievements |
We designed and synthesized a novel receptor targeted against the Gal-beta-1,3-GalNAc disaccharide incorporated liposomal vesicles. We selected a peptidyl bis(boroxole) derivative as the disaccharide recognition moiety of the designed receptor. We have previously constructed target-selective liposomal membrane fusion systems. To develop the functional liposomes with targeting capabilities against the Gal-beta-1,3-GalNAc disaccharide, we prepared the receptor incorporated liposomes and some types of the glyco- or glycosphingo- lipids possessing target liposomes. The membrane fusion behavior was examined by a probe dilution assay based on the fluorescence resonance energy transfer (FRET) between the membrane-bound fluorophores NBD-PE and Rh-PE. The fusion kinetics based on the lipid mixing indicates thatthe designed receptor acts as an effective membrane fusion device toward Gal-beta-1,3-GalNAc disaccharide.
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Report
(4 results)
Research Products
(5 results)
